PMID- 36986141
OWN - NLM
STAT- MEDLINE
DCOM- 20230330
LR  - 20231031
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 15
IP  - 6
DP  - 2023 Mar 15
TI  - Untargeted and Targeted Blood Lipidomic Signature Profile of Gestational Alcohol 
      Exposure.
LID - 10.3390/nu15061411 [doi]
LID - 1411
AB  - Alcohol consumption has a close relationship with blood lipid levels in a 
      nonpregnant state, with a myriad of effects on the liver; however, little is 
      known about the interaction of alcohol and lipids in the context of fetal alcohol 
      spectrum disorders (FASD). We herein aimed to determine the effect of alcohol on 
      the lipid profile in a pregnant rat model, with a focus on FASD. Dry blood spots 
      (50 microL) were obtained from rat maternal blood collected on gestational day (GD) 
      20, two hours after the last binge alcohol exposure (4.5 g/kg, GD 5-10; 6 g/kg, 
      GD 11-20). The samples were then analyzed using high-throughput untargeted and 
      targeted lipid profiling via liquid chromatography-tandem mass spectrometry 
      (LC-MS/MS). In untargeted lipidomics, 73 of 315 identified lipids were altered in 
      the alcohol group compared to the pair-fed controls; 67 were downregulated and 6 
      were upregulated. In targeted analysis, 57 of the 260 studied lipid subspecies 
      were altered, including Phosphatidylcholine (PC), Phosphatidylethanolamine (PE), 
      Phosphatidylglycerol (PG), Phosphatidic Acid (PA), Phosphatidylinositol (PI), and 
      Phosphatidylserine (PS); 36 of these were downregulated and 21 lipid subspecies 
      were upregulated. These findings suggest alcohol-induced dysregulation of lipids 
      in the maternal blood of rats and provide novel insights into possible FASD 
      mechanisms.
FAU - Naik, Vishal D
AU  - Naik VD
AD  - Department of Obstetrics & Gynecology, C.S. Mott Center for Human Growth and 
      Development, School of Medicine, Wayne State University, Detroit, MI 48201, USA.
FAU - Ramadoss, Jayanth
AU  - Ramadoss J
AD  - Department of Obstetrics & Gynecology, C.S. Mott Center for Human Growth and 
      Development, School of Medicine, Wayne State University, Detroit, MI 48201, USA.
AD  - Department of Physiology, School of Medicine, Wayne State University, Detroit, MI 
      48201, USA.
LA  - eng
GR  - R01 HL151497/HL/NHLBI NIH HHS/United States
GR  - R01 AA023520/AA/NIAAA NIH HHS/United States
PT  - Journal Article
DEP - 20230315
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 3K9958V90M (Ethanol)
RN  - 0 (Phosphatidylcholines)
SB  - IM
MH  - Humans
MH  - Pregnancy
MH  - Female
MH  - Rats
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - *Lipidomics
MH  - *Fetal Alcohol Spectrum Disorders
MH  - Tandem Mass Spectrometry/methods
MH  - Ethanol
MH  - Phosphatidylcholines
PMC - PMC10051993
OTO - NOTNLM
OT  - FASD
OT  - alcohol
OT  - pregnancy
OT  - targeted lipidomics
OT  - untargeted
COIS- The authors have no conflict of interest to disclose.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:11
PMCR- 2023/03/15
CRDT- 2023/03/29 01:59
PHST- 2023/02/24 00:00 [received]
PHST- 2023/03/08 00:00 [revised]
PHST- 2023/03/13 00:00 [accepted]
PHST- 2023/03/30 06:11 [medline]
PHST- 2023/03/29 01:59 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/15 00:00 [pmc-release]
AID - nu15061411 [pii]
AID - nutrients-15-01411 [pii]
AID - 10.3390/nu15061411 [doi]
PST - epublish
SO  - Nutrients. 2023 Mar 15;15(6):1411. doi: 10.3390/nu15061411.