PMID- 36986412 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240301 IS - 2076-0817 (Print) IS - 2076-0817 (Electronic) IS - 2076-0817 (Linking) VI - 12 IP - 3 DP - 2023 Mar 21 TI - Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China. LID - 10.3390/pathogens12030490 [doi] LID - 490 AB - The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken. FAU - Hu, Danhe AU - Hu D AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Wu, Chao AU - Wu C AD - Yunnan Institute of Parasitic Diseases, Pu'er 665000, China. FAU - Wang, Ruichen AU - Wang R AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Yao, Xiaohui AU - Yao X AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Nie, Kai AU - Nie K AUID- ORCID: 0000-0002-7963-8431 AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Lv, Quan AU - Lv Q AD - Yunnan Institute of Parasitic Diseases, Pu'er 665000, China. FAU - Fu, Shihong AU - Fu S AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Yin, Qikai AU - Yin Q AUID- ORCID: 0000-0001-8239-0214 AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Su, Wenzhe AU - Su W AUID- ORCID: 0000-0002-6672-0508 AD - Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China. FAU - Li, Fan AU - Li F AUID- ORCID: 0000-0002-8895-2473 AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Xu, Songtao AU - Xu S AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - He, Ying AU - He Y AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Liang, Guodong AU - Liang G AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. FAU - Li, Xiangdong AU - Li X AUID- ORCID: 0000-0002-4635-1602 AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin, College of Life Sciences, Tarim University, Alar 843399, China. FAU - Wang, Huanyu AU - Wang H AUID- ORCID: 0000-0001-6245-2027 AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. LA - eng GR - U01 AI151810/AI/NIAID NIH HHS/United States GR - AI151810/NH/NIH HHS/United States PT - Journal Article DEP - 20230321 PL - Switzerland TA - Pathogens JT - Pathogens (Basel, Switzerland) JID - 101596317 PMC - PMC10058924 OTO - NOTNLM OT - Tembusu virus OT - mosquito-borne flaviviruses OT - phylogeny OT - sequence analysis COIS- The authors declare that they have no conflict of interest. EDAT- 2023/03/30 06:00 MHDA- 2023/03/30 06:01 PMCR- 2023/03/21 CRDT- 2023/03/29 02:01 PHST- 2023/02/14 00:00 [received] PHST- 2023/03/20 00:00 [revised] PHST- 2023/03/20 00:00 [accepted] PHST- 2023/03/30 06:01 [medline] PHST- 2023/03/29 02:01 [entrez] PHST- 2023/03/30 06:00 [pubmed] PHST- 2023/03/21 00:00 [pmc-release] AID - pathogens12030490 [pii] AID - pathogens-12-00490 [pii] AID - 10.3390/pathogens12030490 [doi] PST - epublish SO - Pathogens. 2023 Mar 21;12(3):490. doi: 10.3390/pathogens12030490.