PMID- 36988113
OWN - NLM
STAT- MEDLINE
DCOM- 20230612
LR  - 20231116
IS  - 1098-2299 (Electronic)
IS  - 0272-4391 (Linking)
VI  - 84
IP  - 4
DP  - 2023 Jun
TI  - Cinepazide maleate promotes recovery from spinal cord injury by inhibiting 
      inflammation and prolonging neuronal survival.
PG  - 736-746
LID - 10.1002/ddr.22052 [doi]
AB  - This study aimed to investigate the therapeutic effects of cinepazide maleate 
      (CM) on spinal cord injury (SCI) in rats, thereby providing an experimental basis 
      for the use of CM as a preventative and therapeutic strategy for SCI. Terminal 
      deoxynucleotidyl transferase dUTP nick end labeling staining and western blot 
      analysis were used to assess neural cell apoptosis. enzyme-linked immunosorbent 
      assay was used to analyze the expression of interleukin (IL)-1beta, IL-6, and tumor 
      necrosis factor-alpha (TNF-alpha) in spinal cord tissues and cerebrospinal fluid. CD68 
      staining and western blot analysis were used to investigate the effect of CM on 
      microglia activation. The effects of CM on motor function and histological damage 
      in rats after SCI were investigated using the Basso-Beattie-Bresnahan (BBB) 
      score, footprint assay, hematoxylin and eosin staining, and NeuN staining. In 
      vitro models of neuronal cell injury and microglial inflammation were developed 
      to investigate the effects of CM on apoptosis and inflammation. Functional tests 
      (BBB score and footprint test) revealed that CM-treated rats had significantly 
      improved motor function. In vivo CM treatment reduced the number of apoptotic 
      cells at the site of injury. Similarly, in vitro CM treatment reduced H(2) O(2) 
      -induced neuronal apoptosis. In vivo CM treatment reduced the number of 
      CD68-positive microglia and the expression levels of TNF-alpha, IL-1beta, and IL-6. 
      Similarly, in vitro CM treatment reduced LPS-induced pro-inflammatory cytokines 
      in microglia. CM promotes the recovery of motor function by inhibiting 
      SCI-induced apoptosis and inflammatory responses and reducing the area of the 
      post-SCI cavity in rats. These findings indicate that CM is a potential drug 
      worthy of translational studies for SCI treatment.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Li, Dadi
AU  - Li D
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Zhao, Shuxian
AU  - Zhao S
AD  - Department of Infectious Disease, The Affiliated Fuyang People's Hospital of 
      Anhui Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Zhu, Bin
AU  - Zhu B
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Zhao, Wanmin
AU  - Zhao W
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Ding, Ya
AU  - Ding Y
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Li, Xuejun
AU  - Li X
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Fu, Donglin
AU  - Fu D
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Yu, Haiyang
AU  - Yu H
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Wang, Baogui
AU  - Wang B
AD  - Department of Infectious Disease, The Affiliated Fuyang People's Hospital of 
      Anhui Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
FAU - Pan, Tan
AU  - Pan T
AUID- ORCID: 0009-0009-1760-6803
AD  - Department of Orthopedics, The Affiliated Fuyang People's Hospital of Anhui 
      Medical University, Fuyang People's Hospital, Fuyang, Anhui, China.
LA  - eng
GR  - Health Commission of Anhui province (AHWJ2021b111), Health Commission of Fuyang 
      City (FY2021-002), Anhui Medical University Fund project (2021xkj210), and Key 
      Project of natural sciences in Bengbu Medical College (2022byzd165)./
PT  - Journal Article
DEP - 20230329
PL  - United States
TA  - Drug Dev Res
JT  - Drug development research
JID - 8204468
RN  - 67Y4P5C84X (cinepazide)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - *Spinal Cord Injuries/drug therapy
MH  - Spinal Cord/metabolism/pathology
MH  - Inflammation/metabolism
MH  - Apoptosis
OTO - NOTNLM
OT  - apoptosis
OT  - cinepazide maleate
OT  - inflammation
OT  - spinal cord injury
EDAT- 2023/03/30 06:00
MHDA- 2023/06/12 06:43
CRDT- 2023/03/29 07:03
PHST- 2023/03/03 00:00 [revised]
PHST- 2023/01/08 00:00 [received]
PHST- 2023/03/13 00:00 [accepted]
PHST- 2023/06/12 06:43 [medline]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/29 07:03 [entrez]
AID - 10.1002/ddr.22052 [doi]
PST - ppublish
SO  - Drug Dev Res. 2023 Jun;84(4):736-746. doi: 10.1002/ddr.22052. Epub 2023 Mar 29.