PMID- 36989663
OWN - NLM
STAT- MEDLINE
DCOM- 20230417
LR  - 20230417
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 230
DP  - 2023 Jun 15
TI  - Sequence-specific DNA labelling for fluorescence microscopy.
PG  - 115256
LID - S0956-5663(23)00198-7 [pii]
LID - 10.1016/j.bios.2023.115256 [doi]
AB  - The preservation of nucleus structure during microscopy imaging is a top priority 
      for understanding chromatin organization, genome dynamics, and gene expression 
      regulation. In this review, we summarize the sequence-specific DNA labelling 
      methods that can be used for imaging in fixed and/or living cells without harsh 
      treatment and DNA denaturation: (i) hairpin polyamides, (ii) triplex-forming 
      oligonucleotides, (iii) dCas9 proteins, (iv) transcription activator-like 
      effectors (TALEs) and (v) DNA methyltransferases (MTases). All these techniques 
      are capable of identifying repetitive DNA loci and robust probes are available 
      for telomeres and centromeres, but visualizing single-copy sequences is still 
      challenging. In our futuristic vision, we see gradual replacement of the 
      historically important fluorescence in situ hybridization (FISH) by less invasive 
      and non-destructive methods compatible with live cell imaging. Combined with 
      super-resolution fluorescence microscopy, these methods will open the possibility 
      to look into unperturbed structure and dynamics of chromatin in living cells, 
      tissues and whole organisms.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Pradhan, Shalini
AU  - Pradhan S
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany.
FAU - Apaydin, Sinem
AU  - Apaydin S
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany.
FAU - Bucevicius, Jonas
AU  - Bucevicius J
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany.
FAU - Gerasimaite, Ruta
AU  - Gerasimaite R
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany.
FAU - Kostiuk, Georgij
AU  - Kostiuk G
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany.
FAU - Lukinavicius, Grazvydas
AU  - Lukinavicius G
AD  - Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck 
      Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Gottingen, 
      Germany. Electronic address: grazvydas.lukinavicius@mpinat.mpg.de.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20230321
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 9007-49-2 (DNA)
RN  - 0 (Chromatin)
SB  - IM
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Biosensing Techniques
MH  - DNA/chemistry
MH  - Chromatin/genetics
MH  - Microscopy, Fluorescence/methods
OTO - NOTNLM
OT  - Chromatin imaging
OT  - DNA methyltransferases
OT  - Polyamides
OT  - Transcription activator-like effectors
OT  - Triplex forming oligonucleotides
OT  - dCas9
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/03/30 06:00
MHDA- 2023/04/17 06:41
CRDT- 2023/03/29 18:04
PHST- 2022/12/09 00:00 [received]
PHST- 2023/03/04 00:00 [revised]
PHST- 2023/03/21 00:00 [accepted]
PHST- 2023/04/17 06:41 [medline]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/29 18:04 [entrez]
AID - S0956-5663(23)00198-7 [pii]
AID - 10.1016/j.bios.2023.115256 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2023 Jun 15;230:115256. doi: 10.1016/j.bios.2023.115256. 
      Epub 2023 Mar 21.