PMID- 36992832
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 14
DP  - 2023
TI  - Effect and mechanism of longkui yinxiao soup in treating psoriasis in mice.
PG  - 1136604
LID - 10.3389/fphar.2023.1136604 [doi]
LID - 1136604
AB  - Objective: Longkui Yinxiao Soup is a traditional Chinese medicine formula used to 
      treat psoriasis for decades. Although Longkui Yinxiao Soup showed promising 
      efficacy in clinical practice, the regulatory mechanisms of Longkui Yinxiao Soup 
      remain elusive. This study aimed to explore the underlying mechanisms of Longkui 
      Yinxiao Soup in a psoriasis-like mouse model. Methods: Longkui Yinxiao Soup was 
      quality controlled by determining the contents of imperatorin and rhoifolin using 
      high-performance liquid chromatography. The imiquimod-induced psoriasis-like 
      mouse model was used to study the therapeutic effect and mechanism of Longkui 
      Yinxiao Soup. The histopathological skin changes were observed by hematoxylin and 
      eosin staining; the infiltration of proliferating proteins, proliferating cell 
      nuclear antigen and Ki67, in skin tissues were observed by immunohistochemical 
      analysis; and the inflammatory factors such as interleukin (IL)-6, tumor necrosis 
      factor (TNF)-alpha, IL-23, and IL-17 in serum were detected using enzyme-linked 
      immunosorbent assay. RNA sequencing and bioinformatic analysis were used to 
      predict the mechanism of LYS against psoriasis. mRNA expressions of p38, 
      extracellular regulated protein kinases (ERK), mitogen-activated protein kinase 3 
      (MEK3), mitogen-activated protein kinase 6 (MEK6), RAP1 GTPase activating protein 
      (Rap1gap), and Rap1 were determined using real-time quantitative polymerase chain 
      reaction. The expression levels of proteins related to Rap1-mitogen-activated 
      protein kinase signaling pathways were measured by Western blotting. Results: A 
      quality-control method for Longkui Yinxiao Soup was successfully established 
      using imperatorin and rhoifolin as content determination indexes. Longkui Yinxiao 
      Soup significantly ameliorated the psoriatic symptoms in mice. The serum levels 
      of inflammatory cytokines such as IL-6, TNF-alpha, IL-23, and IL-17 were decreased, 
      and the expression levels of antigen identified by monoclonal antibody Ki67 
      (Ki67) and PCNA in skin tissues were downregulated. Moreover, the inhibition of 
      Rap1-MAPK signaling pathways by Longkui Yinxiao Soup was detected. Conclusion: 
      This study confirmed the antipsoriatic activity of Longkui Yinxiao Soup in 
      psoriasis-like mice. This might be due to the inhibition of inflammatory factor 
      secretion, keratinocyte proliferation, and the Rap1-MAPK signal pathway.
CI  - Copyright (c) 2023 Zhu, Chen, Tai, Pan, Shen, Chen and Zhu.
FAU - Zhu, Congcong
AU  - Zhu C
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Chen, Ya
AU  - Chen Y
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Tai, Zongguang
AU  - Tai Z
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Pan, Huijun
AU  - Pan H
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Shen, Min
AU  - Shen M
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Chen, Zhongjian
AU  - Chen Z
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
FAU - Zhu, Quangang
AU  - Zhu Q
AD  - Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 
      China.
AD  - Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230313
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC10040526
OTO - NOTNLM
OT  - RAP1-MAPK signaling
OT  - RAP1-MAPK signaling inflammation
OT  - inflammation
OT  - keratinocyte proliferation
OT  - longkui yinxiao soup
OT  - psoriasis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/03/31 06:00
MHDA- 2023/03/31 06:01
PMCR- 2023/03/13
CRDT- 2023/03/30 02:46
PHST- 2023/01/03 00:00 [received]
PHST- 2023/02/22 00:00 [accepted]
PHST- 2023/03/31 06:01 [medline]
PHST- 2023/03/30 02:46 [entrez]
PHST- 2023/03/31 06:00 [pubmed]
PHST- 2023/03/13 00:00 [pmc-release]
AID - 1136604 [pii]
AID - 10.3389/fphar.2023.1136604 [doi]
PST - epublish
SO  - Front Pharmacol. 2023 Mar 13;14:1136604. doi: 10.3389/fphar.2023.1136604. 
      eCollection 2023.