PMID- 36995036
OWN - NLM
STAT- MEDLINE
DCOM- 20231204
LR  - 20240119
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 32
IP  - 12
DP  - 2023 Dec
TI  - Altered expression of S100 fused-type proteins in an atopic dermatitis skin 
      model.
PG  - 2160-2165
LID - 10.1111/exd.14797 [doi]
AB  - Atopic dermatitis (AD) is a chronic inflammatory skin disorder with elevated 
      interleukin (IL)-4 and IL-13 signatures and extensive barrier dysfunction, which 
      is correlated with the downregulation of filaggrin (FLG). FLG is a member of the 
      S100 fused-type protein family and this family also includes cornulin (CRNN), 
      filaggrin-2 (FLG2), hornerin (HRNR) repetin (RPTN), trichohyalin (TCHH) and 
      trichohyalin-like 1 (TCHHL1). The present study aimed to examine the effects of 
      IL-4 and IL-13 and the downregulation of FLG on the expression of S100 fused-type 
      proteins using a three-dimensional (3D) AD skin model by immunohistochemical 
      study and quantitative polymerase chain reaction. In the 3D AD skin model, which 
      was generated by a stimulation of recombinant IL-4 and IL-13, the expression of 
      FLG, FLG2, HRNR and TCHH was decreased, while that of RPTN was increased in 
      comparison to the 3D control skin. In the FLG knockdown (KD) 3D skin model, which 
      was generated using FLG siRNA, the expression of HRNR was increased. The 
      expression of the other proteins did not differ to a statistically significant 
      extent. The expression of fused-S100 type protein family members may differ in AD 
      skin. This suggests that these proteins play different roles in the pathogenesis 
      of AD.
CI  - (c) 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Makino, Teruhiko
AU  - Makino T
AUID- ORCID: 0000-0002-0536-1679
AD  - Department of Dermatology, Faculty of Medicine, Academic Assembly, University of 
      Toyama, Toyama, Toyama, Japan.
FAU - Mizawa, Megumi
AU  - Mizawa M
AD  - Department of Dermatology, Faculty of Medicine, Academic Assembly, University of 
      Toyama, Toyama, Toyama, Japan.
FAU - Takemoto, Keita
AU  - Takemoto K
AUID- ORCID: 0000-0002-3036-1451
AD  - Department of Dermatology, Faculty of Medicine, Academic Assembly, University of 
      Toyama, Toyama, Toyama, Japan.
FAU - Yamamoto, Seiji
AU  - Yamamoto S
AD  - Department of Pathology, Faculty of Medicine, Academic Assembly, University of 
      Toyama, Toyama, Toyama, Japan.
FAU - Shimizu, Tadamichi
AU  - Shimizu T
AD  - Department of Dermatology, Faculty of Medicine, Academic Assembly, University of 
      Toyama, Toyama, Toyama, Japan.
LA  - eng
GR  - JP15K09740/Japan Society for the Promotion of Science/
GR  - JP18K08265/Japan Society for the Promotion of Science/
GR  - JP18K16051/Japan Society for the Promotion of Science/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230330
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Filaggrin Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Interleukin-13)
RN  - 0 (S100 Proteins)
RN  - 0 (Intermediate Filament Proteins)
SB  - IM
MH  - Humans
MH  - *Dermatitis, Atopic/metabolism
MH  - Filaggrin Proteins
MH  - Interleukin-4/metabolism
MH  - Interleukin-13/metabolism
MH  - Skin/metabolism
MH  - S100 Proteins/genetics/metabolism
MH  - Intermediate Filament Proteins/genetics/metabolism
OTO - NOTNLM
OT  - S100 fused-type proteins
OT  - atopic dermatitis
OT  - filaggrin
OT  - three-dimensional skin equivalent
OT  - type 2 cytokines
EDAT- 2023/03/31 06:00
MHDA- 2023/12/04 12:43
CRDT- 2023/03/30 08:33
PHST- 2023/03/01 00:00 [revised]
PHST- 2022/12/17 00:00 [received]
PHST- 2023/03/13 00:00 [accepted]
PHST- 2023/12/04 12:43 [medline]
PHST- 2023/03/31 06:00 [pubmed]
PHST- 2023/03/30 08:33 [entrez]
AID - 10.1111/exd.14797 [doi]
PST - ppublish
SO  - Exp Dermatol. 2023 Dec;32(12):2160-2165. doi: 10.1111/exd.14797. Epub 2023 Mar 
      30.