PMID- 36995847
OWN - NLM
STAT- MEDLINE
DCOM- 20230403
LR  - 20230403
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 118
DP  - 2023
TI  - DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and 
      differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) 
      amazonensis promastigotes.
PG  - e220044
LID - S0074-02762023000101104 [pii]
LID - 10.1590/0074-02760220044 [doi]
LID - e220044
AB  - BACKGROUND: Dendritic cells (DCs) specific intercellular adhesion molecule 
      (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania 
      promastigotes mediating its interaction with DC and neutrophils, potentially 
      influencing the infection outcome. OBJECTIVES: In this work, we investigated 
      whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) 
      lesions as well as the in vitro binding pattern of Leishmania (Viannia) 
      braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS: DC-SIGN 
      receptor was labeled by immunohistochemistry in cryopreserved CL tissue 
      fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and 
      RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected 
      (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in 
      co-culture. RESULTS: In CL lesion infiltrate, DC-SIGNPOS cells were present in 
      the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while 
      binding to DC-SIGNNEG was low. La showed precocious and higher affinity to 
      DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these 
      populations. CONCLUSION: Our results demonstrate that DC-SIGN receptor is present 
      in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the 
      differences in the binding pattern to Lb and La suggest DC-SIGN can influence in 
      a difference way the intake of the parasites at the first hours after Leishmania 
      infection. These results raise the hypothesis that DC-SIGN receptor could 
      participate in the immunopathogenesis of American tegumentary leishmaniasis 
      accounting for the differences in the outcome of the Leishmania spp. infection.
FAU - Mendes-Aguiar, Carolina de O
AU  - Mendes-Aguiar CO
AUID- ORCID: 0000-0001-9601-8376
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
AD  - Universidade Federal do Rio Grande do Norte, Instituto de Medicina Tropical do 
      Rio Grande do Norte, Natal, RN, Brasil.
FAU - Kitahara-Oliveira, Milene Yoko
AU  - Kitahara-Oliveira MY
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
FAU - de Almeida, Ana Cristina Oliveira
AU  - de Almeida ACO
AD  - Universidade do Estado do Rio de Janeiro, Faculdade de Ciencias Medicas, 
      Departamento de Microbiologia, Imunologia e Parasitologia, Rio de Janeiro, RJ, 
      Brasil.
FAU - Pereira-Oliveira, Marcia
AU  - Pereira-Oliveira M
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
FAU - de Oliveira Neto, Manoel Paes
AU  - de Oliveira Neto MP
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Pesquisa Clinica em Micobacterioses, Rio de Janeiro, RJ, Brasil.
FAU - Pirmez, Claude
AU  - Pirmez C
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
FAU - Sampaio, Elizabeth Pereira
AU  - Sampaio EP
AD  - National Institute of Allergy and Infectious Diseases, Laboratory of Clinical 
      Immunology and Microbiology, Bethesda, MD, USA.
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio de Hanseniase, 
      Rio de Janeiro, RJ, Brasil.
FAU - Gomes-Silva, Adriano
AU  - Gomes-Silva A
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Pesquisa Clinica em Micobacterioses, Rio de Janeiro, RJ, Brasil.
FAU - Da-Cruz, Alda Maria
AU  - Da-Cruz AM
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratorio 
      Interdisciplinar de Pesquisas Medicas, Rio de Janeiro, RJ, Brasil.
AD  - Universidade do Estado do Rio de Janeiro, Faculdade de Ciencias Medicas, 
      Departamento de Microbiologia, Imunologia e Parasitologia, Rio de Janeiro, RJ, 
      Brasil.
LA  - eng
PT  - Journal Article
DEP - 20230327
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - Humans
MH  - *Leishmania
MH  - *Leishmania braziliensis/metabolism
MH  - *Leishmaniasis, Cutaneous/parasitology
MH  - Cell Adhesion Molecules/metabolism
PMC - PMC10042235
EDAT- 2023/03/31 06:00
MHDA- 2023/04/03 06:42
PMCR- 2023/03/27
CRDT- 2023/03/30 12:13
PHST- 2022/04/11 00:00 [received]
PHST- 2023/02/06 00:00 [accepted]
PHST- 2023/04/03 06:42 [medline]
PHST- 2023/03/30 12:13 [entrez]
PHST- 2023/03/31 06:00 [pubmed]
PHST- 2023/03/27 00:00 [pmc-release]
AID - S0074-02762023000101104 [pii]
AID - 10.1590/0074-02760220044 [doi]
PST - epublish
SO  - Mem Inst Oswaldo Cruz. 2023 Mar 27;118:e220044. doi: 10.1590/0074-02760220044. 
      eCollection 2023.