PMID- 36997584
OWN - NLM
STAT- MEDLINE
DCOM- 20230403
LR  - 20230419
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Mar 30
TI  - Analysis of FCGR3A-p.176Val variants in women with recurrent pregnancy loss and 
      the association with CD16a expression and anti-HLA antibody status.
PG  - 5232
LID - 10.1038/s41598-023-32156-9 [doi]
LID - 5232
AB  - Natural Killer (NK) cells have been implicated in recurrent pregnancy loss (RPL). 
      The p.Val176Phe (or Val158Phe) Single Nucleotide Polymorphism (SNP) in the FCGR3A 
      gene encoding the FcgammaRIIIA or CD16a receptor has been associated with an enhanced 
      affinity for IgG and stronger NK-mediated antibody-dependent cellular 
      cytotoxicity. We hypothesized that the presence of at least one p.176Val variant 
      associates with RPL and increased CD16a expression and alloantibodies e.g., 
      against paternal human leukocyte antigen (HLA). In 50 women with RPL, we studied 
      frequencies of the p.Val176Phe FCGR3A polymorphisms. Additionally, CD16a 
      expression and anti-HLA antibody status were analyzed by flowcytometry and 
      Luminex Single Antigens. In woman with RPL, frequencies were: 20% (VV), 42% (VF) 
      and 38% (FF). This was comparable to frequencies from the European population in 
      the NCBI SNP database and in an independent Dutch cohort of healthy women. NK 
      cells from RPL women with a VV (22,575 [18731-24607]) and VF (24,294 
      [20157-26637]) polymorphism showed a higher expression of the CD16a receptor than 
      NK cells from RPL women with FF (17,367 [13257-19730]). No difference in 
      frequencies of the FCGR3A-p.176 SNP were detected when comparing women with or 
      without class I and class II anti-HLA antibodies. Our study does not provide 
      strong evidence for an association between the p.Val176Phe FCGR3A SNP and RPL.
CI  - (c) 2023. The Author(s).
FAU - Habets, Denise H J
AU  - Habets DHJ
AD  - Department of Obstetrics and Gynecology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands. denise.habets@maastrichtuniversity.nl.
AD  - Department of Transplantation Immunology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands. denise.habets@maastrichtuniversity.nl.
AD  - GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The 
      Netherlands. denise.habets@maastrichtuniversity.nl.
FAU - Al-Nasiry, Salwan
AU  - Al-Nasiry S
AD  - Department of Obstetrics and Gynecology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands.
AD  - GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The 
      Netherlands.
FAU - Nagelkerke, Sietse Q
AU  - Nagelkerke SQ
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Amsterdam University Medical Centre, University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Department of Pediatric Immunology, Rheumatology and Infectious Diseases, Emma 
      Children's Hospital, Amsterdam University Medical Centre, University of 
      Amsterdam, Amsterdam, The Netherlands.
FAU - Voorter, Christina E M
AU  - Voorter CEM
AD  - Department of Transplantation Immunology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands.
AD  - GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The 
      Netherlands.
FAU - Spaanderman, Marc E A
AU  - Spaanderman MEA
AD  - Department of Obstetrics and Gynecology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands.
AD  - GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The 
      Netherlands.
FAU - Kuijpers, Taco W
AU  - Kuijpers TW
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Amsterdam University Medical Centre, University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Department of Pediatric Immunology, Rheumatology and Infectious Diseases, Emma 
      Children's Hospital, Amsterdam University Medical Centre, University of 
      Amsterdam, Amsterdam, The Netherlands.
FAU - Wieten, Lotte
AU  - Wieten L
AD  - Department of Transplantation Immunology, Maastricht University Medical Centre, 
      Maastricht, The Netherlands.
AD  - GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The 
      Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230330
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Receptors, IgG)
RN  - 0 (Antibodies)
RN  - 0 (HLA Antigens)
RN  - 0 (FCGR3A protein, human)
SB  - IM
MH  - Pregnancy
MH  - Humans
MH  - Female
MH  - *Receptors, IgG/metabolism
MH  - Killer Cells, Natural
MH  - Polymorphism, Single Nucleotide
MH  - Antibodies/metabolism
MH  - HLA Antigens/metabolism
MH  - *Abortion, Habitual
PMC - PMC10063683
COIS- The authors declare no competing interests.
EDAT- 2023/03/31 06:00
MHDA- 2023/04/03 06:42
PMCR- 2023/03/30
CRDT- 2023/03/30 23:18
PHST- 2022/08/02 00:00 [received]
PHST- 2023/03/23 00:00 [accepted]
PHST- 2023/04/03 06:42 [medline]
PHST- 2023/03/30 23:18 [entrez]
PHST- 2023/03/31 06:00 [pubmed]
PHST- 2023/03/30 00:00 [pmc-release]
AID - 10.1038/s41598-023-32156-9 [pii]
AID - 32156 [pii]
AID - 10.1038/s41598-023-32156-9 [doi]
PST - epublish
SO  - Sci Rep. 2023 Mar 30;13(1):5232. doi: 10.1038/s41598-023-32156-9.