PMID- 37000227
OWN - NLM
STAT- MEDLINE
DCOM- 20230418
LR  - 20230418
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 107
IP  - 9
DP  - 2023 May
TI  - Isolation and physiological properties of methanogenic archaea that degrade 
      tetramethylammonium hydroxide.
PG  - 3047-3056
LID - 10.1007/s00253-023-12488-2 [doi]
AB  - Tetramethylammonium hydroxide (TMAH) is a known toxic chemical used in the 
      photolithography process of semiconductor photoelectronic processes. Significant 
      amounts of wastewater containing TMAH are discharged from electronic industries. 
      It is therefore attractive to apply anaerobic treatment to industrial wastewater 
      containing TMAH. In this study, a novel TMAH-degrading methanogenic archaeon was 
      isolated from the granular sludge of a psychrophilic upflow anaerobic sludge 
      blanket (UASB) reactor treating synthetic wastewater containing TMAH. Although 
      the isolate (strain NY-STAYD) was phylogenetically related to 
      Methanomethylovorans uponensis, it was the only isolated Methanomethylovorans 
      strain capable of TMAH degradation. Strain NY-STAYD was capable of degrading 
      methylamine compounds, similar to the previously isolated Methanomethylovorans 
      spp. While the strain was able to grow at temperatures ranging from 15 to 37 degrees C, 
      the cell yield was higher at lower temperatures. The distribution of archaeal 
      cells affiliated with the genus Methanomethylovorans in the original granular 
      sludge was investigated by fluorescence in situ hybridization (FISH) using 
      specific oligonucleotide probe targeting 16S rRNA. The results demonstrated that 
      the TMAH-degrading cells associated with the genus Methanomethylovorans were not 
      intermingled with other microorganisms but rather isolated on the granule's 
      surface as a lone dominant archaeon. KEY POINTS: * A TMAH-degrading methanogenic 
      Methanomethylovorans strain was isolated * This strain was the only known 
      Methanomethylovorans isolate that can degrade TMAH * The highest cell yield of 
      the isolate was obtained at psychrophilic conditions.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Iguchi, Akinori
AU  - Iguchi A
AUID- ORCID: 0000-0002-4870-0693
AD  - Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life 
      Sciences (NUPALS), 265-1, Higashijima, Akiha-ku, Niigata City, Niigata, 956-8603, 
      Japan.
FAU - Takemura, Yasuyuki
AU  - Takemura Y
AD  - Regional Environment Conservation Division, National Institute for Environmental 
      Studies (NIES), 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
FAU - Danshita, Tsuyoshi
AU  - Danshita T
AD  - Department of Civil Engineering and Architecture, National Institute for 
      Technology, Tokuyama College, 3538 Gakuendai, Shunan, Yamaguchi, 745-8585, Japan.
FAU - Kurihara, Takuya
AU  - Kurihara T
AD  - Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life 
      Sciences (NUPALS), 265-1, Higashijima, Akiha-ku, Niigata City, Niigata, 956-8603, 
      Japan.
FAU - Aoki, Masataka
AU  - Aoki M
AD  - Regional Environment Conservation Division, National Institute for Environmental 
      Studies (NIES), 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.
FAU - Hori, Saori
AU  - Hori S
AD  - Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life 
      Sciences (NUPALS), 265-1, Higashijima, Akiha-ku, Niigata City, Niigata, 956-8603, 
      Japan.
FAU - Shigematsu, Toru
AU  - Shigematsu T
AD  - Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life 
      Sciences (NUPALS), 265-1, Higashijima, Akiha-ku, Niigata City, Niigata, 956-8603, 
      Japan.
FAU - Syutsubo, Kazuaki
AU  - Syutsubo K
AUID- ORCID: 0000-0003-4181-2171
AD  - Regional Environment Conservation Division, National Institute for Environmental 
      Studies (NIES), 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan. 
      stubo@nies.go.jp.
AD  - Research Center for Water Environment Technology, School of Engineering, The 
      University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. 
      stubo@nies.go.jp.
LA  - eng
PT  - Journal Article
DEP - 20230331
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Wastewater)
RN  - 0 (Sewage)
RN  - H0W55235FC (tetramethylammonium)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Archaea/genetics/metabolism
MH  - Wastewater
MH  - Sewage/chemistry
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics/metabolism
MH  - Bioreactors
MH  - *Euryarchaeota/metabolism
MH  - Methanosarcinaceae/genetics
MH  - Anaerobiosis
MH  - Waste Disposal, Fluid/methods
OTO - NOTNLM
OT  - Anaerobic wastewater treatment
OT  - Isolation
OT  - Methanogen
OT  - Methanomethylovorans
OT  - Tetramethylammonium hydroxide (TMAH)
OT  - UASB
EDAT- 2023/04/01 06:00
MHDA- 2023/04/18 10:16
CRDT- 2023/03/31 11:14
PHST- 2022/12/08 00:00 [received]
PHST- 2023/03/17 00:00 [accepted]
PHST- 2023/03/15 00:00 [revised]
PHST- 2023/04/18 10:16 [medline]
PHST- 2023/04/01 06:00 [pubmed]
PHST- 2023/03/31 11:14 [entrez]
AID - 10.1007/s00253-023-12488-2 [pii]
AID - 10.1007/s00253-023-12488-2 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2023 May;107(9):3047-3056. doi: 
      10.1007/s00253-023-12488-2. Epub 2023 Mar 31.