PMID- 37001147
OWN - NLM
STAT- MEDLINE
DCOM- 20230509
LR  - 20240210
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 24
IP  - 5
DP  - 2023 May 8
TI  - Biomimetic SARS-CoV-2 Spike Protein Nanoparticles.
PG  - 2030-2041
LID - 10.1021/acs.biomac.2c01465 [doi]
AB  - COVID-19 is an infectious respiratory disease caused by the severe acute 
      respiratory syndrome coronavirus 2 (SARS-CoV-2). This virus contains a crucial 
      coat protein that engages with target cells via a receptor binding domain (RBD) 
      on its spike protein. To better study the RBD and its therapeutic opportunities, 
      we genetically engineered a simple fusion with a thermo-responsive elastin-like 
      polypeptide (ELP). These fusions express in Escherichia coli at a high yield in 
      the soluble fraction and were easily purified using ELP-mediated phase separation 
      (79 mg/L culture). Interestingly, they assembled peptide-based nanoparticles 
      (R(h) = 71.4 nm), which was attributed to oligomerization of RBDs (25.3 kDa) 
      counterbalanced by steric stabilization by a soluble ELP (73.4 kDa). To 
      investigate their biophysical properties, we explored the size, shape, and 
      binding affinity for the human angiotensin-converting enzyme 2 (hACE2) and 
      cellular uptake. Biomimetic nanoparticles such as these may enable future 
      strategies to target the same cells, tissues, and cell-surface receptors as those 
      harnessed by SARS-CoV-2.
FAU - Phan, Alvin
AU  - Phan A
AD  - Department of Pharmacology and Pharmaceutical Sciences, USC Alfred E. Mann School 
      of Pharmacy and Pharmaceutical Sciences, University of Southern California, 1985 
      Zonal Avenue, Los Angeles, California 90089, United States.
FAU - Avila, Hugo
AU  - Avila H
AUID- ORCID: 0000-0003-3742-589X
AD  - Department of Pharmacology and Pharmaceutical Sciences, USC Alfred E. Mann School 
      of Pharmacy and Pharmaceutical Sciences, University of Southern California, 1985 
      Zonal Avenue, Los Angeles, California 90089, United States.
FAU - MacKay, J Andrew
AU  - MacKay JA
AUID- ORCID: 0000-0002-3626-1654
AD  - Department of Pharmacology and Pharmaceutical Sciences, USC Alfred E. Mann School 
      of Pharmacy and Pharmaceutical Sciences, University of Southern California, 1985 
      Zonal Avenue, Los Angeles, California 90089, United States.
AD  - Department of Ophthalmology, Keck School of Medicine of USC, University of 
      Southern California, 1450 San Pablo Street, Los Angeles, California 90033, United 
      States.
AD  - Alfred E. Mann Department of Biomedical Engineering, USC Viterbi School of 
      Engineering, University of Southern California, 1042 Downey Way, Los Angeles, 
      California 90089, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230331
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (spike protein, SARS-CoV-2)
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Humans
MH  - *COVID-19
MH  - Spike Glycoprotein, Coronavirus/genetics/metabolism
MH  - SARS-CoV-2
MH  - Receptors, Virus/chemistry/metabolism
MH  - Biomimetics
MH  - Protein Binding
MH  - *Nanoparticles
EDAT- 2023/04/01 06:00
MHDA- 2023/05/09 10:16
CRDT- 2023/03/31 17:23
PHST- 2023/05/09 10:16 [medline]
PHST- 2023/04/01 06:00 [pubmed]
PHST- 2023/03/31 17:23 [entrez]
AID - 10.1021/acs.biomac.2c01465 [doi]
PST - ppublish
SO  - Biomacromolecules. 2023 May 8;24(5):2030-2041. doi: 10.1021/acs.biomac.2c01465. 
      Epub 2023 Mar 31.