PMID- 37001427
OWN - NLM
STAT- MEDLINE
DCOM- 20230418
LR  - 20230418
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 338
DP  - 2023 Jul 15
TI  - Microbially induced calcium precipitation driven by denitrification: Performance, 
      metabolites, and molecular mechanisms.
PG  - 117826
LID - S0301-4797(23)00614-X [pii]
LID - 10.1016/j.jenvman.2023.117826 [doi]
AB  - Microbially induced calcium precipitation (MICP) driven by denitrification has 
      attracted extensive attention due to its application potential in nitrate removal 
      from calcium-rich groundwater. However, little research has been conducted on 
      this technique at the molecular level. Here, Pseudomonas WZ39 was used to explore 
      the molecular mechanisms of nitrate-dependent MICP and the effects of Ca(2+) on 
      bacterial transcriptional regulation and metabolic response. The results 
      exhibited that appropriate Ca(2+) concentration (4.5 mM) can promote 
      denitrification and the production of ATP, EPSs, and SMPs. Genome-wide analysis 
      showed that the nitrate-dependent MICP was accomplished through heterotrophic 
      denitrification and CO(2) capture. During this process, EPS biosynthesis and 
      Ca(2+) signaling regulation were involved in the nucleation template supply and 
      Ca(2+) homeostasis balance. Untargeted transcriptome- and metabolome-association 
      analyses revealed that the addition of Ca(2+) triggered the significant 
      up-regulation in several key pathways, such as transmembrane transporter and 
      channel activities, amino acid metabolism, fatty acid biosynthesis, and carbon 
      metabolism, which played a momentous role in the mineral nucleation and energy 
      provision. The detailed information provided novel insights for understanding the 
      active control of bacteria on MICP, and has great significance for deepening the 
      cognition of groundwater remediation using nitrate-dependent MICP technique.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Wang, Zhao
AU  - Wang Z
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
FAU - Su, Junfeng
AU  - Su J
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China. Electronic address: sjf1977518@sina.com.
FAU - Ali, Amjad
AU  - Ali A
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
FAU - Gao, Zhihong
AU  - Gao Z
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
FAU - Zhang, Ruijie
AU  - Zhang R
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
FAU - Li, Yifei
AU  - Li Y
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
FAU - Yang, Wenshuo
AU  - Yang W
AD  - School of Environmental and Municipal Engineering, Xi'an University of 
      Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of 
      Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 
      710055, China.
LA  - eng
PT  - Journal Article
DEP - 20230329
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - SY7Q814VUP (Calcium)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 0 (Nitrates)
SB  - IM
MH  - *Calcium/metabolism
MH  - *Calcium Carbonate/chemistry
MH  - Nitrates
MH  - Denitrification
MH  - Bacteria/metabolism
MH  - Chemical Precipitation
OTO - NOTNLM
OT  - Genome-wide
OT  - Metabolites
OT  - Metabolome
OT  - Nitrate-dependent MICP
OT  - Transcriptome
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/04/01 06:00
MHDA- 2023/04/18 06:42
CRDT- 2023/03/31 18:09
PHST- 2022/12/22 00:00 [received]
PHST- 2023/02/25 00:00 [revised]
PHST- 2023/03/26 00:00 [accepted]
PHST- 2023/04/18 06:42 [medline]
PHST- 2023/04/01 06:00 [pubmed]
PHST- 2023/03/31 18:09 [entrez]
AID - S0301-4797(23)00614-X [pii]
AID - 10.1016/j.jenvman.2023.117826 [doi]
PST - ppublish
SO  - J Environ Manage. 2023 Jul 15;338:117826. doi: 10.1016/j.jenvman.2023.117826. 
      Epub 2023 Mar 29.