PMID- 37002232
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230610
IS  - 2731-6068 (Electronic)
IS  - 2731-6068 (Linking)
VI  - 9
IP  - 1
DP  - 2023 Mar 31
TI  - Aging reduces calreticulin expression and alters spontaneous calcium signals in 
      astrocytic endfeet of the mouse dorsolateral striatum.
PG  - 5
LID - 10.1038/s41514-023-00102-8 [doi]
LID - 5
AB  - Aging-related impairment of the blood brain barrier (BBB) and neurovascular unit 
      (NVU) increases the risk for neurodegeneration. Among various cells that 
      participate in BBB and NVU function, calcium signals in astrocytic endfeet are 
      crucial for maintaining BBB and NVU integrity. To assess if aging is associated 
      with altered calcium signals within astrocytic endfeet of the dorsolateral 
      striatum (DLS), we expressed GCaMP6f in DLS astrocytes of young (3-4 months), 
      middle-aged (12-15 months) and aging (20-30 months) mice. Compared to endfeet in 
      young mice, DLS endfeet in aging mice demonstrated decreased calreticulin 
      expression, and alterations to both spontaneous membrane-associated and 
      mitochondrial calcium signals. While young mice required both extracellular and 
      endoplasmic reticulum calcium sources for endfoot signals, middle-aged and aging 
      mice showed heavy dependence on endoplasmic reticulum calcium. Thus, astrocytic 
      endfeet show significant changes in calcium buffering and sources throughout the 
      lifespan, which is important for understanding mechanisms by which aging impairs 
      the BBB and NVU.
CI  - (c) 2023. The Author(s).
FAU - Zarate, Sara M
AU  - Zarate SM
AD  - Department of Neuroscience & Experimental Therapeutics, Texas A&M University 
      School of Medicine, 8447 Riverside Pkwy, Bryan, TX, 77807, USA.
FAU - Huntington, Taylor E
AU  - Huntington TE
AD  - Department of Neuroscience & Experimental Therapeutics, Texas A&M University 
      School of Medicine, 8447 Riverside Pkwy, Bryan, TX, 77807, USA.
AD  - Texas A&M Institute for Neuroscience (TAMIN), Texas A&M University, College 
      Station, TX, 77843, USA.
FAU - Bagher, Pooneh
AU  - Bagher P
AUID- ORCID: 0000-0002-9778-6200
AD  - Department of Cellular and Integrative Physiology, University of Nebraska Medical 
      Center, Omaha, NE, 68198, USA.
FAU - Srinivasan, Rahul
AU  - Srinivasan R
AUID- ORCID: 0000-0003-0237-6602
AD  - Department of Neuroscience & Experimental Therapeutics, Texas A&M University 
      School of Medicine, 8447 Riverside Pkwy, Bryan, TX, 77807, USA. rahul@tamu.edu.
AD  - Texas A&M Institute for Neuroscience (TAMIN), Texas A&M University, College 
      Station, TX, 77843, USA. rahul@tamu.edu.
LA  - eng
GR  - R01 NS115809/NS/NINDS NIH HHS/United States
GR  - R01 HL155618/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20230331
PL  - England
TA  - NPJ Aging
JT  - npj aging
JID - 9918402285106676
PMC - PMC10066375
COIS- The authors declare no competing interests.
EDAT- 2023/04/01 06:00
MHDA- 2023/04/01 06:01
PMCR- 2023/03/31
CRDT- 2023/03/31 23:16
PHST- 2022/05/01 00:00 [received]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/04/01 06:01 [medline]
PHST- 2023/03/31 23:16 [entrez]
PHST- 2023/04/01 06:00 [pubmed]
PHST- 2023/03/31 00:00 [pmc-release]
AID - 10.1038/s41514-023-00102-8 [pii]
AID - 102 [pii]
AID - 10.1038/s41514-023-00102-8 [doi]
PST - epublish
SO  - NPJ Aging. 2023 Mar 31;9(1):5. doi: 10.1038/s41514-023-00102-8.