PMID- 37002243 OWN - NLM STAT- MEDLINE DCOM- 20230404 LR - 20230420 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 14 IP - 1 DP - 2023 Mar 31 TI - Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype. PG - 1820 LID - 10.1038/s41467-023-37527-4 [doi] LID - 1820 AB - Immune and inflammatory responses have an important function in the pathophysiology of pulmonary hypertension (PH). However, little is known about the immune landscape in peripheral circulation in patients with high-altitude pulmonary hypertension (HAPH). We apply single-cell transcriptomics to characterize the monocytes that are significantly enriched in the peripheral blood mononuclear cells (PBMC) of HAPH patients. We discover an increase in C1 (non-classical) and C2 (intermediate) monocytes in PBMCs and a decrease in hypoxia-inducible transcription factor-1alpha (HIF-1alpha) in all monocyte subsets associated with HAPH. In addition, we demonstrate that similar immune adaptations may exist in HAPH and PH. Overall, we characterize an immune cell atlas of the peripheral blood in HAPH patients. Our data provide evidence that specific monocyte subsets and HIF-1alpha downregulation might be implicated in the pathogenesis of HAPH. CI - (c) 2023. The Author(s). FAU - Wu, Xin-Hua AU - Wu XH AUID- ORCID: 0000-0003-4625-9635 AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - He, Yang-Yang AU - He YY AUID- ORCID: 0000-0002-3517-572X AD - School of Pharmacy, Henan University, Henan, China. FAU - Chen, Zhang-Rong AU - Chen ZR AUID- ORCID: 0000-0003-4666-8412 AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. AD - Department of Cardiology, The Affiliated Hospital of Guizhou Medical University, Guizhou, China. FAU - He, Ze-Yuan AU - He ZY AD - Department of Cardiology, Yulong People's Hospital, Yunnan, China. FAU - Yan, Yi AU - Yan Y AUID- ORCID: 0000-0003-2860-3252 AD - Heart Center and Shanghai Institute of Pediatric Congenital Heart Disease, Shanghai Children's Medical Center, National Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - He, Yangzhige AU - He Y AD - Department of Medical Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Wang, Guang-Ming AU - Wang GM AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Dong, Yu AU - Dong Y AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Yang, Ying AU - Yang Y AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Sun, Yi-Min AU - Sun YM AD - CapitalBio Technology Corporation, Beijing, China. FAU - Ren, Yong-Hong AU - Ren YH AD - CapitalBio Technology Corporation, Beijing, China. FAU - Zhao, Qiu-Yan AU - Zhao QY AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Yang, Xiao-Dan AU - Yang XD AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Wang, Li-Ying AU - Wang LY AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Fu, Cai-Jun AU - Fu CJ AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - He, Miao AU - He M AD - Institute of Pharmacy, Dali University, Yunnan, China. FAU - Zhang, Si-Jin AU - Zhang SJ AD - Department of Cardiology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Fu, Ji-Fen AU - Fu JF AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. FAU - Liu, Hong AU - Liu H AUID- ORCID: 0000-0001-7358-8683 AD - Department of Cardiology; Yunnan Provincial Engineering Research Center of Prevention and Treatment of Trans-plateau Cardiovascular Diseases, The First Affiliated Hospital of Dali University, Yunnan, China. daliliuhong@163.com. FAU - Jing, Zhi-Cheng AU - Jing ZC AUID- ORCID: 0000-0003-0493-0929 AD - Department of Cardiology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. jingzhicheng@vip.163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230331 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - Pulmonary edema of mountaineers SB - IM MH - Humans MH - *Hypertension, Pulmonary/etiology MH - Altitude MH - Monocytes MH - Leukocytes, Mononuclear MH - Phenotype MH - Single-Cell Analysis PMC - PMC10066231 COIS- The authors declare no competing interests. EDAT- 2023/04/01 06:00 MHDA- 2023/04/04 06:42 PMCR- 2023/03/31 CRDT- 2023/03/31 23:16 PHST- 2021/08/26 00:00 [received] PHST- 2023/03/21 00:00 [accepted] PHST- 2023/04/04 06:42 [medline] PHST- 2023/03/31 23:16 [entrez] PHST- 2023/04/01 06:00 [pubmed] PHST- 2023/03/31 00:00 [pmc-release] AID - 10.1038/s41467-023-37527-4 [pii] AID - 37527 [pii] AID - 10.1038/s41467-023-37527-4 [doi] PST - epublish SO - Nat Commun. 2023 Mar 31;14(1):1820. doi: 10.1038/s41467-023-37527-4.