PMID- 37002923
OWN - NLM
STAT- MEDLINE
DCOM- 20231004
LR  - 20231005
IS  - 2211-1247 (Electronic)
VI  - 42
IP  - 4
DP  - 2023 Apr 25
TI  - Hic1 identifies a specialized mesenchymal progenitor population in the embryonic 
      limb responsible for bone superstructure formation.
PG  - 112325
LID - S2211-1247(23)00336-4 [pii]
LID - 10.1016/j.celrep.2023.112325 [doi]
AB  - The musculoskeletal system relies on the integration of multiple components with 
      diverse physical properties, such as striated muscle, tendon, and bone, that 
      enable locomotion and structural stability. This relies on the emergence of 
      specialized, but poorly characterized, interfaces between these various elements 
      during embryonic development. Within the appendicular skeleton, we show that a 
      subset of mesenchymal progenitors (MPs), identified by Hic1, do not contribute to 
      the primary cartilaginous anlagen but represent the MP population, whose progeny 
      directly contribute to the interfaces that connect bone to tendon (entheses), 
      tendon to muscle (myotendinous junctions), and the associated superstructures. 
      Furthermore, deletion of Hic1 leads to skeletal defects reflective of deficient 
      muscle-bone coupling and, consequently, perturbation of ambulation. Collectively, 
      these findings show that Hic1 identifies a unique MP population that contributes 
      to a secondary wave of bone sculpting critical to skeletal morphogenesis.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Arostegui, Martin
AU  - Arostegui M
AD  - Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 
      1Z3, Canada; Department of Cellular and Physiological Sciences, University of 
      British Columbia, Vancouver, BC V6T 1Z3, Canada.
FAU - Scott, R Wilder
AU  - Scott RW
AD  - Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 
      1Z3, Canada; Department of Cellular and Physiological Sciences, University of 
      British Columbia, Vancouver, BC V6T 1Z3, Canada; School of Biomedical 
      Engineering, University of British Columbia, 2222 Health Sciences Mall, 
      Vancouver, BC V6T 1Z3, Canada.
FAU - Underhill, T Michael
AU  - Underhill TM
AD  - Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 
      1Z3, Canada; Department of Cellular and Physiological Sciences, University of 
      British Columbia, Vancouver, BC V6T 1Z3, Canada; School of Biomedical 
      Engineering, University of British Columbia, 2222 Health Sciences Mall, 
      Vancouver, BC V6T 1Z3, Canada. Electronic address: tunderhi@brc.ubc.ca.
LA  - eng
GR  - MOP-10684/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230331
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
SB  - IM
MH  - *Tendons
MH  - *Muscle, Skeletal
MH  - Osteogenesis
MH  - Bone and Bones
MH  - Cartilage
OTO - NOTNLM
OT  - CP: Developmental biology
OT  - chondrogenesis
OT  - congenital limb defects
OT  - enthesis
OT  - limb development
OT  - lineage tracing
OT  - mesenchymal progenitors
OT  - myotendinous junction
OT  - single-cell omics
OT  - skeletal development
OT  - transgenic models
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/04/02 06:00
MHDA- 2023/10/04 06:43
CRDT- 2023/04/01 12:36
PHST- 2022/08/10 00:00 [received]
PHST- 2022/12/21 00:00 [revised]
PHST- 2023/03/17 00:00 [accepted]
PHST- 2023/10/04 06:43 [medline]
PHST- 2023/04/02 06:00 [pubmed]
PHST- 2023/04/01 12:36 [entrez]
AID - S2211-1247(23)00336-4 [pii]
AID - 10.1016/j.celrep.2023.112325 [doi]
PST - ppublish
SO  - Cell Rep. 2023 Apr 25;42(4):112325. doi: 10.1016/j.celrep.2023.112325. Epub 2023 
      Mar 31.