PMID- 37005841
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230404
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 6
DP  - 2023 Mar
TI  - [Effects of Rehmanniae Radix and Rehmanniae Radix Praeparata on proteomics and 
      autophagy in mice with type 2 diabetes mellitus induced by high-fat diet coupled 
      with streptozotocin].
PG  - 1535-1545
LID - 10.19540/j.cnki.cjcmm.20220901.301 [doi]
AB  - To compare the pancreatic proteomics and autophagy between Rehmanniae Radix-and 
      Rehmanniae Radix Praeparata-treated mice with type 2 diabetes mellitus(T2DM). The 
      T2DM mouse model was established by high-fat diet coupled with 
      streptozotocin(STZ, intraperitoneal injection, 100 mg.kg~(-1), once a day for 
      three consecutive days). The mice were then randomly assigned into a control 
      group, low-(5 g.kg~(-1)) and high-dose(15 g.kg~(-1)) Rehmanniae Radix groups, 
      low-(150 mg.kg~(-1)) and high-dose(300 mg.kg~(-1)) catalpol groups, low-(5 
      g.kg~(-1)) and high-dose(15 g.kg~(-1)) Rehmanniae Radix Praeparata groups, 
      low-(150 mg.kg~(-1)) and high-dose(300 mg.kg~(-1)) 5-hydroxymethyl 
      furfuraldehyde(5-HMF) groups, and a metformin(250 mg.kg~(-1)) group. In addition, 
      a normal group was also set and each group included 8 mice. The pancreas was 
      collected after four weeks of administration and proteomics tools were employed 
      to study the effects of Rehmanniae Radix and Rehmanniae Radix Praeparata on 
      protein expression in the pancreas of T2DM mice. The expression levels of 
      proteins involved in autophagy, inflammation, and oxidative stress response in 
      the pancreatic tissues of T2DM mice were determined by western blotting, 
      immunohistochemical assay, and transmission electron microscopy. The results 
      showed that the differential proteins between the model group and Rehmanniae 
      Radix/Rehmanniae Radix Prae-parata group were enriched in 7 KEGG pathways, such 
      as autophagy-animal, which indicated that the 7 pathways may be associated with 
      T2DM. Compared with the control group, drug administration significantly 
      up-regulated the expression levels of beclin1 and phosphorylated mammalian target 
      of rapamycin(p-mTOR)/mTOR and down-regulated those of the inflammation 
      indicators, Toll-like receptor-4(TLR4) and Nod-like receptor protein 3(NLRP3), in 
      the pancreas of T2DM mice, and Rehmanniae Radix showed better performance. In 
      addition, the expression levels of inducible nitric oxide synthase(iNOS), nuclear 
      factor erythroid 2-related factor 2(Nrf2), and heine oxygenase-1(HO-1) in the 
      pancreas of T2DM mice were down-regulated after drug administration, and 
      Rehmanniae Radix Praeparata demonstrated better performance. The results indicate 
      that both Rehmanniae Radix and Rehmanniae Radix Praeparata can alleviate the 
      inflammatory symptoms, reduce oxidative stress response, and increase the 
      autophagy level in the pancreas of T2DM mice, while they exert the effect on 
      different autophagy pathways.
FAU - Yan, Jing-Ning
AU  - Yan JN
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Liu, Xiao-Qin
AU  - Liu XQ
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China Shanxi 
      Key Laboratory of Chinese Herbal Medicine Processing,Shanxi University of Chinese 
      Medicine Jinzhong 030619,China.
FAU - Meng, Xiang-Long
AU  - Meng XL
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China Shanxi 
      Key Laboratory of Chinese Herbal Medicine Processing,Shanxi University of Chinese 
      Medicine Jinzhong 030619,China.
FAU - Ren, Ke-le
AU  - Ren KL
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China Shanxi 
      Key Laboratory of Chinese Herbal Medicine Processing,Shanxi University of Chinese 
      Medicine Jinzhong 030619,China.
FAU - Wu, Xue-Min
AU  - Wu XM
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Wang, Hai-Qin
AU  - Wang HQ
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Wang, Hong-Liang
AU  - Wang HL
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Sheng, Qi
AU  - Sheng Q
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Li, Bin
AU  - Li B
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Zhang, Ding-Bang
AU  - Zhang DB
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Chen, Hong-Zhou
AU  - Chen HZ
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Zhang, Fa-Yun
AU  - Zhang FY
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Li, Ming-Hao
AU  - Li MH
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China.
FAU - Zhang, Shuo-Sheng
AU  - Zhang SS
AD  - the First Clinical College & College of Chinese Medicine and Food 
      Engineering,Shanxi University of Chinese Medicine Jinzhong 030619,China Shanxi 
      Key Laboratory of Chinese Herbal Medicine Processing,Shanxi University of Chinese 
      Medicine Jinzhong 030619,China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 1BEM3U6LQQ (rehmannia root)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/drug therapy/genetics
MH  - Streptozocin/pharmacology
MH  - Diet, High-Fat/adverse effects
MH  - Proteomics
MH  - Inflammation
MH  - TOR Serine-Threonine Kinases
MH  - Autophagy
MH  - Mammals
OTO - NOTNLM
OT  - Rehmanniae Radix
OT  - Rehmanniae Radix Praeparata
OT  - autophagy
OT  - proteomics
OT  - type 2 diabetes mellitus
EDAT- 2023/04/04 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/04/03 02:23
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/04/03 02:23 [entrez]
PHST- 2023/04/04 06:00 [pubmed]
AID - 10.19540/j.cnki.cjcmm.20220901.301 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Mar;48(6):1535-1545. doi: 
      10.19540/j.cnki.cjcmm.20220901.301.