PMID- 37006001
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230404
IS  - 1998-3603 (Electronic)
IS  - 0970-9290 (Linking)
VI  - 33
IP  - 4
DP  - 2022 Oct-Dec
TI  - Assessment of proliferation, clonogenic assay, and osteogenic differentiation of 
      human periodontal ligament stem cells following application of orthodontic 
      forces.
PG  - 382-387
LID - 10.4103/ijdr.ijdr_554_22 [doi]
AB  - CONTEXT: The proliferation and differentiation of human periodontal ligament stem 
      cells (hPDLSC) into other cell types are also mediated by mechanical stresses; 
      they might offer therapeutic benefits in tissue regeneration and angiogenesis. 
      OBJECTIVES: The study was planned to assess the proliferation, clonogenic 
      potential, and osteogenic differentiation of human periodontal ligament stem 
      cells (PDLSC) following the application of light and heavy orthodontic forces. 
      MATERIALS AND METHODS: A couple forces of 50 gm (light force) were applied on the 
      1(st) premolar on the one side and 250 gm (heavy force) on the contralateral side 
      in the upper arch of patients requiring orthodontic treatment with extraction of 
      all 1(st) premolars. After 30 days, periodontal tissues were scrapped from 
      extracted teeth for the establishment of PDLSC in vitro. PDLC from the lower 
      premolar teeth where no orthodontic force was applied acted as the control group. 
      Morphology, viability, proliferating rate and population doubling time, 
      clonogenicity, and alkaline phosphatase activity were analysed. RESULT: The 
      osteogenic potential was confirmed by Alizarin red staining and the expression of 
      the osteogenic markers by qRT-PCR. The morphology, growth kinetics, potency, and 
      osteogenic lineage characteristics inferred the application of high force reduced 
      the proliferative ability and osteogenesis of PDLSC, though the difference was 
      not significant. CONCLUSION: The established PDLSCs demonstrated their MSC-like 
      properties based on morphology, growth kinetics, colony forming ability, and AP 
      activity. The culture-expanded PDLSCs showed their differentiation potential into 
      osteocytes. The application of high force reduced the proliferative ability and 
      osteogenesis of PDLSCs, variations were not significant.
FAU - Kuberan, Shanthi
AU  - Kuberan S
AD  - Department of Orthodontics and Dentofacial Orthopaedics, A.B. Shetty Memorial 
      Institute of Dental Sciences, Nitte (Deemed to be University), Deralakatte, 
      Mangaluru, Karnataka, India.
FAU - Ravi, Marakini Subrahmanya
AU  - Ravi MS
AD  - Department of Orthodontics and Dentofacial Orthopaedics, A.B. Shetty Memorial 
      Institute of Dental Sciences, Nitte (Deemed to be University), Deralakatte, 
      Mangaluru, Karnataka, India.
FAU - Manjappa, Akshay Bairapura
AU  - Manjappa AB
AD  - Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. 
      Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte, Mangaluru, 
      Karnataka, India.
FAU - Rao, Shama
AU  - Rao S
AD  - Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. 
      Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte, Mangaluru, 
      Karnataka, India.
FAU - Basavarajappa, Mohana Kumar
AU  - Basavarajappa MK
AD  - Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. 
      Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte, Mangaluru, 
      Karnataka, India.
FAU - Shetty, Veena
AU  - Shetty V
AD  - Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. 
      Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte, Mangaluru, 
      Karnataka, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Dent Res
JT  - Indian journal of dental research : official publication of Indian Society for 
      Dental Research
JID - 9202990
MH  - Humans
MH  - *Osteogenesis
MH  - *Periodontal Ligament
MH  - Stem Cells
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
OTO - NOTNLM
OT  - Clonogenic assay
OT  - orthodontic force
OT  - osteogenic differentiation
OT  - periodontal ligament stem cells
COIS- None
EDAT- 2023/04/04 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/04/03 02:48
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/04/03 02:48 [entrez]
PHST- 2023/04/04 06:00 [pubmed]
AID - IndianJDentRes_2022_33_4_382_372910 [pii]
AID - 10.4103/ijdr.ijdr_554_22 [doi]
PST - ppublish
SO  - Indian J Dent Res. 2022 Oct-Dec;33(4):382-387. doi: 10.4103/ijdr.ijdr_554_22.