PMID- 37008119
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230404
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 8
IP  - 12
DP  - 2023 Mar 28
TI  - Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette 
      Smoke Extracts on Human Gingival Epithelial Cells.
PG  - 10919-10929
LID - 10.1021/acsomega.2c07324 [doi]
AB  - Smoking increases the risk of a number of diseases, including cardiovascular, 
      oral, and lung diseases. E-cigarettes are gaining popularity among young people 
      as an alternative to cigarettes, but there is debate over whether they are less 
      harmful to the mouth than e-cigarettes. In this study, human gingival epithelial 
      cells (HGECs) were treated with four commercially available e-cigarette aerosol 
      condensates (ECAC) or commercially available generic cigarette smoke condensates 
      (CSC) with different nicotine concentrations. Cell viability was determined by 
      MTT assay. Cell apoptosis was observed by acridine orange (AO) and Hoechst33258 
      staining. The levels of type I collagen, matrix metalloproteinase (MMP-1, MMP-3), 
      cyclooxygenase 2, and inflammatory factors were detected by ELISA and RT-PCR. 
      Finally, ROS levels were analyzed by ROS staining. The different effects of CSC 
      and ECAC on HGECs were compared. The results showed that higher nicotine 
      concentration of CS significantly reduced the activity of HGECs. By contrast, all 
      ECAC had no significant effect. The levels of matrix metalloproteinase, COX-2, 
      and inflammatory factors were higher in HGECs treated with CSC than those treated 
      with ECAC. In contrast, the level of type I collagen was higher in HGECs treated 
      with ECAC than those treated with CSC. In conclusion, all four flavors of 
      e-cigarettes were less toxic to HGE cells than tobacco, but further clinical 
      studies are needed to determine whether e-cigarettes are less harmful to oral 
      health than conventional cigarettes.
CI  - (c) 2023 The Authors. Published by American Chemical Society.
FAU - Su, Le
AU  - Su L
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
AD  - Shengshengxiangrong (Shandong) Biotechnology Co., Ltd., Jinan 250000, P.R. China.
FAU - Liu, Jilong
AU  - Liu J
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
FAU - Yue, Qiulin
AU  - Yue Q
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
AD  - Shengshengxiangrong (Shandong) Biotechnology Co., Ltd., Jinan 250000, P.R. China.
FAU - Zhang, Song
AU  - Zhang S
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
FAU - Zhao, Chen
AU  - Zhao C
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
FAU - Sun, Xin
AU  - Sun X
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
FAU - Xu, Jing
AU  - Xu J
AD  - Shenzhen RELX Tech. Co., Ltd, Shenzhen 518000, China.
FAU - Jiang, Xingtao
AU  - Jiang X
AD  - Shenzhen RELX Tech. Co., Ltd, Shenzhen 518000, China.
FAU - Li, Kunlun
AU  - Li K
AD  - Shandong Zhuoran Biotechnology Co., Ltd., Jinan 250000, P.R. China.
FAU - Li, Baojun
AU  - Li B
AD  - Shandong Zhuoran Biotechnology Co., Ltd., Jinan 250000, P.R. China.
FAU - Zhao, Lin
AU  - Zhao L
AUID- ORCID: 0000-0002-4016-3176
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan 250353, P.R. China.
AD  - Shandong Chenzhang Biotechnology Co., Ltd., Jinan 250353, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20230314
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC10061507
COIS- The authors declare no competing financial interest.
EDAT- 2023/04/04 06:00
MHDA- 2023/04/04 06:01
PMCR- 2023/03/14
CRDT- 2023/04/03 04:07
PHST- 2022/11/15 00:00 [received]
PHST- 2023/03/03 00:00 [accepted]
PHST- 2023/04/04 06:01 [medline]
PHST- 2023/04/03 04:07 [entrez]
PHST- 2023/04/04 06:00 [pubmed]
PHST- 2023/03/14 00:00 [pmc-release]
AID - 10.1021/acsomega.2c07324 [doi]
PST - epublish
SO  - ACS Omega. 2023 Mar 14;8(12):10919-10929. doi: 10.1021/acsomega.2c07324. 
      eCollection 2023 Mar 28.