PMID- 37015350
OWN - NLM
STAT- MEDLINE
DCOM- 20230529
LR  - 20230609
IS  - 1530-8022 (Electronic)
IS  - 0885-3282 (Linking)
VI  - 37
IP  - 10
DP  - 2023 May
TI  - Effect of size and morphology of functionalized upconversion nanoparticle labeled 
      probe on stem cell absorption.
PG  - 1823-1834
LID - 10.1177/08853282231153725 [doi]
AB  - In order to improve the application of stem cell therapy, it is important to 
      monitor the proliferation and differentiation of transplanted stem cells in real 
      time, high sensitivity and high precision. Upconversion nanoparticles (UCNPs) 
      have been employed as bioimaging agents and delivery vehicles for gene 
      therapeutics in several types of cells. Herein, we fabricated multiple functional 
      upconversion nanoparticles (FUC-NPs) as exogenous contrast agents to investigate 
      the effect of FUC-NPs size on the efficiency of tracking human umbilical cord 
      mesenchymal stem cells (HUCMSCs). We used TEM, DLS, MTT, etc. systematically 
      detected the effects of nanoparticle dose and exposure time on HUCMSCs 
      cytotoxicity. Our data showed that incubation of FUC-NPs, HUCMSCs are able to 
      maintain their viability and differentiation ability. Interestingly, the small 
      size FUC-NPs examined, S-FUCs, had a much higher uptake capability than the other 
      FUC-NPs (B-FUCs, H-FUCs and R-FUCs).The study will aid in UCNPs for the 
      engineering of stem cell application in the future.
FAU - Liu, Yanan
AU  - Liu Y
AD  - Shenzhen Longhua Maternity and Child Healthcare Hospital, Shenzhen, China.
FAU - Zheng, Chuping
AU  - Zheng C
AD  - College of Chemistry and Materials Science, Jinan University, Guangzhou, China.
FAU - Liu, Jie
AU  - Liu J
AUID- ORCID: 0000-0003-2237-9309
AD  - College of Chemistry and Materials Science, Jinan University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230404
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Contrast Media)
SB  - IM
MH  - Humans
MH  - *Nanoparticles/toxicity
MH  - *Mesenchymal Stem Cells
MH  - Contrast Media/pharmacology
MH  - Cell Differentiation
MH  - Stem Cell Transplantation
OTO - NOTNLM
OT  - Upconversion nanoparticles
OT  - cellular uptake
OT  - mesenchymal stem cells
OT  - size and morphology
OT  - stem cell differentiation
EDAT- 2023/04/05 06:00
MHDA- 2023/05/29 06:42
CRDT- 2023/04/04 19:53
PHST- 2023/05/29 06:42 [medline]
PHST- 2023/04/05 06:00 [pubmed]
PHST- 2023/04/04 19:53 [entrez]
AID - 10.1177/08853282231153725 [doi]
PST - ppublish
SO  - J Biomater Appl. 2023 May;37(10):1823-1834. doi: 10.1177/08853282231153725. Epub 
      2023 Apr 4.