PMID- 37017890
OWN - NLM
STAT- MEDLINE
DCOM- 20230615
LR  - 20230615
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 48
IP  - 8
DP  - 2023 Aug
TI  - Activation of Ventral Pallidum CaMKIIa-Expressing Neurons Promotes Wakefulness.
PG  - 2502-2513
LID - 10.1007/s11064-023-03915-x [doi]
AB  - The ventral pallidum (VP) is involved in the regulation of a variety of behaviors 
      such as motor, reward, and behavioral motivation, and the ability to perform 
      these functions properly is dependent on a high degree of wakefulness. It is 
      unknown whether VP CaMKIIa-expression (VP(CaMKIIa)) neurons also have a role in 
      sleep-wake regulation and related neuronal circuit mechanisms. In the present 
      experiment, we first used in vivo fiber photometry to find the population 
      activity of VP(CaMKIIa) neurons which increased during the transitions from 
      non-rapid-eye movement (NREM) sleep to wakefulness and NREM sleep to 
      rapid-eye-movement (REM) sleep, with decreased during the transitions from 
      wakefulness to NREM sleep. Then chemogenetic activation of VP(CaMKIIa) neurons 
      induced an increase in wakefulness that lasted for 2 h. Mice that were exposed to 
      short-term optogenetic stimulation woke up quickly from stable NREM sleep, and 
      long-term optogenetic stimulation maintained wakefulness. In addition, 
      optogenetic activation of the axons of VP(CaMKIIa) neurons in the lateral 
      habenula (LHb) also facilitated the initiation and maintenance of wakefulness and 
      mediated anxiety-like behavior. Finally, the method of chemogenetic inhibition 
      was employed to suppress VP(CaMKIIa) neurons, and yet, inhibition of VP(CaMKIIa) 
      neuronal activity did not result in an increase in NREM sleep and a decrease in 
      wakefulness. Overall, our data illustrate that the activation of VP(CaMKIIa) 
      neurons is of great importance for promoting wakefulness.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Li, Yue
AU  - Li Y
AD  - Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, 
      Wuhan, 430071, People's Republic of China.
FAU - Zhang, Xuefen
AU  - Zhang X
AD  - Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, 
      Wuhan, 430071, People's Republic of China.
FAU - Li, Ying
AU  - Li Y
AD  - Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, 
      Wuhan, 430071, People's Republic of China.
FAU - Li, Yidan
AU  - Li Y
AD  - Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, 
      Wuhan, 430071, People's Republic of China.
FAU - Xu, Haibo
AU  - Xu H
AD  - Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, 
      Wuhan, 430071, People's Republic of China. xuhaibo@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230405
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Wakefulness/physiology
MH  - *Basal Forebrain
MH  - Neurons/physiology
MH  - Sleep/physiology
MH  - Sleep, REM
OTO - NOTNLM
OT  - Anxiety-like behavior
OT  - CaMKIIa-expression neurons
OT  - Lateral habenula
OT  - Sleep-wake regulation
OT  - Ventral pallidum
EDAT- 2023/04/06 06:00
MHDA- 2023/06/15 06:42
CRDT- 2023/04/05 11:20
PHST- 2022/11/18 00:00 [received]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/02/11 00:00 [revised]
PHST- 2023/06/15 06:42 [medline]
PHST- 2023/04/06 06:00 [pubmed]
PHST- 2023/04/05 11:20 [entrez]
AID - 10.1007/s11064-023-03915-x [pii]
AID - 10.1007/s11064-023-03915-x [doi]
PST - ppublish
SO  - Neurochem Res. 2023 Aug;48(8):2502-2513. doi: 10.1007/s11064-023-03915-x. Epub 
      2023 Apr 5.