PMID- 37017919
OWN - NLM
STAT- MEDLINE
DCOM- 20230925
LR  - 20230925
IS  - 1720-8386 (Electronic)
IS  - 0391-4097 (Linking)
VI  - 46
IP  - 10
DP  - 2023 Oct
TI  - Hsa_circ_0003928 regulates the progression of diabetic nephropathy through 
      miR-136-5p/PAQR3 axis.
PG  - 2103-2114
LID - 10.1007/s40618-023-02061-z [doi]
AB  - BACKGROUND: Diabetic nephropathy (DN) is one of the complications of diabetes and 
      has a high mortality, but its specific pathogenesis is not clear. In recent 
      years, researches on the mechanism of circRNAs in DN have been proved a lot, 
      whereas the functional mechanism of circ_0003928 in DN remains open and it must 
      be investigated to value its important role in DN prevention. METHODS: HK-2 cells 
      were treated with high glucose (HG), normal glucose (NG) or Mannitol. Cell 
      counting kit-8 (CCK8) and 5-ethynyl-2'-deoxyuridine (EdU) assays were performed 
      to detect cell proliferation. Enzyme-linked immunosorbent assay (ELISA) was 
      applied to analyze malondialdehyde (MDA) and superoxide dismutase 1 (SOD) levels. 
      Flow cytometry and western blot were preformed to measure cell apoptosis. 
      Real-time quantitative PCR (RT-qPCR) was used to test the levels of circ_0003928, 
      miR-136-5p and progestin and adipoQ receptor family member 3 (PAQR3) mRNA. 
      Western blot was executed to detect Bcl2 associated X (Bax), B cell 
      leukemia/lymphoma 2 (Bcl2), smooth muscle (alphaSMA), apolipoprotein (C-IV) and PAQR3 
      levels. Luciferase reporter assay and RNA pull-down assay were used to analyze 
      the target relationship between miR-136-5p and circ_0003928 or PAQR3. RESULTS: 
      Circ_0003928 and PAQR3 expression were up-regulated, whereas miR-136-5p was 
      decreased in DN serum and HG-induced HK-2 cells. Circ_0003928 knockdown promoted 
      cell proliferation, and inhibit cell apoptosis, oxidative stress, and fibrosis in 
      HK-2 cells under HG condition. MiR-136-5p silencing overturned the protective 
      effects of si-circ_0003928 on HG-induced HK-2 cells. MiR-136-5p was targeted by 
      circ_0003928 and directly targeted PAQR3. Overexpression of PAQR3 counteracted 
      the inhibitory functions of circ_0003928 knockdown or miR-136-5p overexpression 
      on HG-induced HK-2 cell injury. CONCLUSION: Circ_0003928 acted as a sponge of 
      miR-136-5p to up-regulating PAQR3 expression, and then regulate the 
      proliferation, oxidative stress, fibrosis and apoptosis in HG-induced HK-2 cells.
CI  - (c) 2023. The Author(s), under exclusive licence to Italian Society of 
      Endocrinology (SIE).
FAU - Zhang, W
AU  - Zhang W
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
FAU - Zhang, L
AU  - Zhang L
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
FAU - Dong, Q
AU  - Dong Q
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
FAU - Wang, X
AU  - Wang X
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
FAU - Li, Z
AU  - Li Z
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
FAU - Wang, Q
AU  - Wang Q
AD  - Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 
      Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. 
      wangqy187@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230405
PL  - Italy
TA  - J Endocrinol Invest
JT  - Journal of endocrinological investigation
JID - 7806594
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN136 microRNA, human)
SB  - IM
MH  - Humans
MH  - *Diabetic Nephropathies/genetics
MH  - Apoptosis
MH  - Biological Assay
MH  - Glucose
MH  - *MicroRNAs/genetics
MH  - *Diabetes Mellitus
OTO - NOTNLM
OT  - Circ_0003928
OT  - Diabetic nephropathy
OT  - PAQR3
OT  - miR-136-5p
EDAT- 2023/04/06 06:00
MHDA- 2023/09/25 06:42
CRDT- 2023/04/05 11:21
PHST- 2022/08/19 00:00 [received]
PHST- 2023/03/06 00:00 [accepted]
PHST- 2023/09/25 06:42 [medline]
PHST- 2023/04/06 06:00 [pubmed]
PHST- 2023/04/05 11:21 [entrez]
AID - 10.1007/s40618-023-02061-z [pii]
AID - 10.1007/s40618-023-02061-z [doi]
PST - ppublish
SO  - J Endocrinol Invest. 2023 Oct;46(10):2103-2114. doi: 10.1007/s40618-023-02061-z. 
      Epub 2023 Apr 5.