PMID- 37019242
OWN - NLM
STAT- MEDLINE
DCOM- 20230523
LR  - 20230523
IS  - 1872-7913 (Electronic)
IS  - 0924-8579 (Linking)
VI  - 61
IP  - 6
DP  - 2023 Jun
TI  - Anti-Porphyromonas gingivalis nanotherapy for maintaining bacterial homeostasis 
      in periodontitis.
PG  - 106801
LID - S0924-8579(23)00082-1 [pii]
LID - 10.1016/j.ijantimicag.2023.106801 [doi]
AB  - Periodontitis is caused by oral flora imbalance, which leads to immune imbalance. 
      Porphyromonas gingivalis is a keystone pathogen in periodontitis, causing the 
      blooming of inflammophilic microbes, and becoming dormant to resist antibiotics. 
      Targeted interventions are needed to destroy this pathogen and collapse its 
      inflammophilic flora. Therefore, a targeting nanoagent antibody-conjugated 
      liposomal drug carrier with ginsenoside Rh2 (A-L-R) was developed for pleiotropic 
      benefits. A-L-R showed high quality in high-performance liquid chromatography 
      (HPLC), Fourier transform infrared (FTIR), and transmission electron microscope 
      (TEM) detection. Only P. gingivalis was influenced by A-L-R, as shown by 
      live/dead cell staining and a series of antimicrobial effects assays. With 
      fluorescence in situ hybridization (FISH) staining and in propidium 
      monoazide-quantitative polymerase chain reaction (PMA-qPCR), the clearance of P. 
      gingivalis by A-L-R was more than for other groups, and only the proportion of P. 
      gingivalis was reduced by A-L-R in monospecies culture. Moreover, in a 
      periodontitis model, A-L-R targeted P. gingivalis with high efficiency and low 
      toxicity, maintaining homeostasis with a relatively stable oral microflora. This 
      targeting nanomedicine offers new strategies for periodontitis therapy, providing 
      a foundation for the prevention and treatment of periodontitis.
CI  - Copyright (c) 2023 Elsevier Ltd and International Society of Antimicrobial 
      Chemotherapy. All rights reserved.
FAU - Chen, Rourong
AU  - Chen R
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Ji, Yaoting
AU  - Ji Y
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Li, Ting
AU  - Li T
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Zhao, Boxuan
AU  - Zhao B
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Guo, Haiying
AU  - Guo H
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Wang, Zijun
AU  - Wang Z
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Yao, Hantao
AU  - Yao H
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Zhang, Zicheng
AU  - Zhang Z
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Liu, Chang
AU  - Liu C
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China. Electronic 
      address: liuc0728@whu.edu.cn.
FAU - Du, Minquan
AU  - Du M
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China. Electronic 
      address: duminquan@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230403
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Humans
MH  - *Porphyromonas gingivalis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Periodontitis/drug therapy/microbiology/prevention & control
MH  - Anti-Bacterial Agents/therapeutic use/pharmacology
MH  - Homeostasis
OTO - NOTNLM
OT  - Periodontitis
OT  - Porphyromonas gingivalis
OT  - antibody conjugates
OT  - liposomes ginsenoside Rh2
OT  - targeted therapies
EDAT- 2023/04/06 06:00
MHDA- 2023/05/23 06:42
CRDT- 2023/04/05 19:24
PHST- 2022/12/08 00:00 [received]
PHST- 2023/03/21 00:00 [revised]
PHST- 2023/03/27 00:00 [accepted]
PHST- 2023/05/23 06:42 [medline]
PHST- 2023/04/06 06:00 [pubmed]
PHST- 2023/04/05 19:24 [entrez]
AID - S0924-8579(23)00082-1 [pii]
AID - 10.1016/j.ijantimicag.2023.106801 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2023 Jun;61(6):106801. doi: 
      10.1016/j.ijantimicag.2023.106801. Epub 2023 Apr 3.