PMID- 37021573
OWN - NLM
STAT- MEDLINE
DCOM- 20230526
LR  - 20230825
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 43
IP  - 6
DP  - 2023 Jun
TI  - Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing 
      Exosomal miR-182-5p.
PG  - 995-1014
LID - 10.1161/ATVBAHA.123.319026 [doi]
AB  - BACKGROUND: Insufficient or disrupted sleep increases the risk of cardiovascular 
      disease, including atherosclerosis. However, we know little about the molecular 
      mechanisms by which sleep modulates atherogenesis. This study aimed to explore 
      the potential role of circulating exosomes in endothelial inflammation and 
      atherogenesis under sleep deprivation status and the molecular mechanisms 
      involved. METHODS: Circulating exosomes were isolated from the plasma of 
      volunteers with or without sleep deprivation and mice subjected to 12-week sleep 
      deprivation or control littermates. miRNA array was performed to determine 
      changes in miRNA expression in circulating exosomes. RESULTS: Although the total 
      circulating exosome levels did not change significantly, the isolated plasma 
      exosomes from sleep-deprived mice or human were a potent inducer of endothelial 
      inflammation and atherogenesis. Through profiling and functional analysis of the 
      global microRNA in the exosomes, we found miR-182-5p is a key exosomal cargo that 
      mediates the proinflammatory effects of exosomes by upregulation of MYD88 
      (myeloid differentiation factor 88) and activation of NF-kB (nuclear factor 
      kappa-B)/NLRP3 pathway in endothelial cells. Moreover, sleep deprivation or the 
      reduction of melatonin directly decreased the synthesis of miR-182-5p and led to 
      the accumulation of reactive oxygen species in small intestinal epithelium. 
      CONCLUSIONS: The findings illustrate an important role for circulating exosomes 
      in distant communications, suggesting a new mechanism underlying the link between 
      sleep disorder and cardiovascular disease.
FAU - Li, Xiao
AU  - Li X
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Cao, Ying
AU  - Cao Y
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Xu, Xinxin
AU  - Xu X
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Wang, Chongyue
AU  - Wang C
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Ni, Qingbin
AU  - Ni Q
AD  - Hydrogen medicine center, Tai 'an City Central Hospital, China (Q.N.).
FAU - Lv, Xiang
AU  - Lv X
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Yang, Chao
AU  - Yang C
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Zhang, Zhaoqiang
AU  - Zhang Z
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
FAU - Qi, Xufeng
AU  - Qi X
AUID- ORCID: 0000-0002-5911-071X
AD  - Key Laboratory of Regenerative Medicine of Ministry of Education, Department of 
      Developmental & Regenerative Biology, Jinan University, Guangzhou, China (X.Q.).
FAU - Song, Guohua
AU  - Song G
AUID- ORCID: 0000-0002-3799-8420
AD  - School of Basic Medical Sciences, and The Second Affiliated Hospital of Shandong 
      First Medical University & Shandong Academy of Medical Science, Jinan, China 
      (X.L., Y.C., X.X., C.W., X.L., C.Y., Z.Z., G.S.).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230406
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn182 microRNA, human)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - Endothelial Cells/metabolism
MH  - Sleep Deprivation/complications/genetics/metabolism
MH  - *Cardiovascular Diseases/metabolism
MH  - *MicroRNAs/metabolism
MH  - *Exosomes/genetics/metabolism
MH  - Inflammation/genetics/metabolism
MH  - *Atherosclerosis/genetics/metabolism
OTO - NOTNLM
OT  - atherogenesis
OT  - endothelial inflammation
OT  - exosomes
OT  - melatonin
OT  - sleep deprivation
COIS- Disclosures None.
EDAT- 2023/04/07 06:00
MHDA- 2023/05/26 06:42
CRDT- 2023/04/06 05:03
PHST- 2023/05/26 06:42 [medline]
PHST- 2023/04/07 06:00 [pubmed]
PHST- 2023/04/06 05:03 [entrez]
AID - 10.1161/ATVBAHA.123.319026 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2023 Jun;43(6):995-1014. doi: 
      10.1161/ATVBAHA.123.319026. Epub 2023 Apr 6.