PMID- 37023566
OWN - NLM
STAT- MEDLINE
DCOM- 20230502
LR  - 20230502
IS  - 2772-9508 (Electronic)
IS  - 2772-9508 (Linking)
VI  - 149
DP  - 2023 Jun
TI  - Additively manufactured polyethylene terephthalate scaffolds for scapholunate 
      interosseous ligament reconstruction.
PG  - 213397
LID - S2772-9508(23)00120-6 [pii]
LID - 10.1016/j.bioadv.2023.213397 [doi]
AB  - The regeneration of the ruptured scapholunate interosseous ligament (SLIL) 
      represents a clinical challenge. Here, we propose the use of a Bone-Ligament-Bone 
      (BLB) 3D-printed polyethylene terephthalate (PET) scaffold for achieving 
      mechanical stabilisation of the scaphoid and lunate following SLIL rupture. The 
      BLB scaffold featured two bone compartments bridged by aligned fibres (ligament 
      compartment) mimicking the architecture of the native tissue. The scaffold 
      presented tensile stiffness in the range of 260 +/- 38 N/mm and ultimate load of 
      113 +/- 13 N, which would support physiological loading. A finite element analysis 
      (FEA), using inverse finite element analysis (iFEA) for material property 
      identification, showed an adequate fit between simulation and experimental data. 
      The scaffold was then biofunctionalized using two different methods: injected 
      with a Gelatin Methacryloyl solution containing human mesenchymal stem cell 
      spheroids (hMSC) or seeded with tendon-derived stem cells (TDSC) and placed in a 
      bioreactor to undergo cyclic deformation. The first approach demonstrated high 
      cell viability, as cells migrated out of the spheroid and colonised the 
      interstitial space of the scaffold. These cells adopted an elongated morphology 
      suggesting the internal architecture of the scaffold exerted topographical 
      guidance. The second method demonstrated the high resilience of the scaffold to 
      cyclic deformation and the secretion of a fibroblastic related protein was 
      enhanced by the mechanical stimulation. This process promoted the expression of 
      relevant proteins, such as Tenomodulin (TNMD), indicating mechanical stimulation 
      may enhance cell differentiation and be useful prior to surgical implantation. In 
      conclusion, the PET scaffold presented several promising characteristics for the 
      immediate mechanical stabilisation of disassociated scaphoid and lunate and, in 
      the longer-term, the regeneration of the ruptured SLIL.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Gomez-Cerezo, M Natividad
AU  - Gomez-Cerezo MN
AD  - School of Dentistry, Centre for Orofacial Regeneration, Reconstruction and 
      Rehabilitation (COR3), The University of Queensland, Herston, QLD, Australia.
FAU - Perevoshchikova, Nataliya
AU  - Perevoshchikova N
AD  - Griffith Centre of Biomedical and Rehabilitation Engineering (GCORE), Griffith 
      University, Gold Coast, QLD 4222, Australia.
FAU - Ruan, Rui
AU  - Ruan R
AD  - Centre for Orthopaedic Research, The UWA Medical School, The University of 
      Western Australia, Crawley, WA 6009, Australia.
FAU - Moerman, Kevin M
AU  - Moerman KM
AD  - Biomechanics Research Centre, National University of Ireland Galway, Galway, 
      Ireland.
FAU - Bindra, Randy
AU  - Bindra R
AD  - Griffith Centre of Biomedical and Rehabilitation Engineering (GCORE), Griffith 
      University, Gold Coast, QLD 4222, Australia; School of Medicine, Griffith 
      University, Gold Coast, QLD 4215, Australia.
FAU - Lloyd, David G
AU  - Lloyd DG
AD  - Griffith Centre of Biomedical and Rehabilitation Engineering (GCORE), Griffith 
      University, Gold Coast, QLD 4222, Australia.
FAU - Zheng, Ming Hao
AU  - Zheng MH
AD  - Centre for Orthopaedic Research, The UWA Medical School, The University of 
      Western Australia, Crawley, WA 6009, Australia; Perron Institute for Neurological 
      and Translational Science, Perth, Western Australia 6009, Australia; Australian 
      Research Council Centre for Personalised Therapeutics Technologies, Australia.
FAU - Saxby, David J
AU  - Saxby DJ
AD  - Griffith Centre of Biomedical and Rehabilitation Engineering (GCORE), Griffith 
      University, Gold Coast, QLD 4222, Australia.
FAU - Vaquette, Cedryck
AU  - Vaquette C
AD  - School of Dentistry, Centre for Orofacial Regeneration, Reconstruction and 
      Rehabilitation (COR3), The University of Queensland, Herston, QLD, Australia. 
      Electronic address: c.vaquette@uq.edu.au.
LA  - eng
PT  - Journal Article
DEP - 20230321
PL  - Netherlands
TA  - Biomater Adv
JT  - Biomaterials advances
JID - 9918383886206676
RN  - 0 (Polyethylene Terephthalates)
SB  - IM
MH  - Humans
MH  - Polyethylene Terephthalates
MH  - Ligaments, Articular/surgery/physiology
MH  - *Scaphoid Bone/surgery
MH  - *Lunate Bone/surgery
MH  - Wrist Joint
OTO - NOTNLM
OT  - 3D-printing
OT  - Hand surgery
OT  - Polyethylene terephthalate (PET)
OT  - Scapholunate interosseous ligament
OT  - Tissue-engineering
COIS- Declaration of competing interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: Cedryck Vaquette reports financial support was provided by MTPConnect 
      Biomedical Technology Horizons. Cedryck Vaquette reports a relationship with 
      MTPConnect Biomedical Technology Horizons that includes: funding grants. Cedryck 
      Vaquette, Randy Bindra have a patent #PCT/AU2018/000133 issued to U.S. Patent and 
      Trademark Office.
EDAT- 2023/04/07 06:00
MHDA- 2023/05/02 06:42
CRDT- 2023/04/06 18:04
PHST- 2022/11/28 00:00 [received]
PHST- 2023/03/13 00:00 [revised]
PHST- 2023/03/17 00:00 [accepted]
PHST- 2023/05/02 06:42 [medline]
PHST- 2023/04/07 06:00 [pubmed]
PHST- 2023/04/06 18:04 [entrez]
AID - S2772-9508(23)00120-6 [pii]
AID - 10.1016/j.bioadv.2023.213397 [doi]
PST - ppublish
SO  - Biomater Adv. 2023 Jun;149:213397. doi: 10.1016/j.bioadv.2023.213397. Epub 2023 
      Mar 21.