PMID- 37024792 OWN - NLM STAT- MEDLINE DCOM- 20230410 LR - 20230411 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 24 IP - 1 DP - 2023 Apr 6 TI - Gonadal transcriptomes reveal sex-biased expression genes associated with sex determination and differentiation in red-tail catfish (Hemibagrus wyckioides). PG - 183 LID - 10.1186/s12864-023-09264-x [doi] LID - 183 AB - BACKGROUND: Red-tail catfish (Hemibagrus wyckioides) is an important commercially farmed catfish in southern China. Males of red-tail catfish grow faster than females, suggesting that all-male catfish will produce more significant economic benefits in aquaculture practice. However, little research has been reported on sex determination and gonadal development in red-tail catfish. RESULTS: In this study, we performed the first transcriptomic analysis of male and female gonads at four developmental stages at 10, 18, 30, and 48 days post hatching (dph) using RNA-seq technology. A total of 23,588 genes were screened in 24 sequenced samples, of which 28, 213, 636, and 1381 differentially expressed genes (DEGs) were detected at four developmental stages, respectively. Seven candidate genes of sex determination and differentiation were further identified. Real-time quantitative PCR (RT-qPCR) further confirmed that anti-Mullerian hormone (amh), growth differentiation factor 6a (gdf6a), testis-specific gene antigen 10 (tsga10), and cytochrome P450 family 17 subfamily A (cyp17a) were highly expressed mainly in the male, while cytochrome P450 family 19 subfamily A polypeptide 1b (cyp19a1b), forkhead box L2 (foxl2), and hydroxysteroid 17-beta dehydrogenase 1 (hsd17b1) were highly expressed in the female. The KEGG pathway enrichment data showed that these identified DEGs were mainly involved in steroid hormone biosynthesis and TGF-beta signaling pathways. CONCLUSIONS: Based on RNA-seq data of gonads at the early developmental stages, seven DEGs shared by the four developmental stages were identified, among which amh and gdf6a may be the male-biased expression genes, while foxl2, cyp19a1b and hsd17b1 may be the female-biased expression genes in red-tail catfish. Our study will provide crucial genetic information for the research on sex control in red-tail catfish, as well as for exploring the evolutionary processes of sex determination mechanisms in fish. CI - (c) 2023. The Author(s). FAU - Wei, Wen-Yu AU - Wei WY AD - College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China. AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. FAU - Gong, Yi AU - Gong Y AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Guo, Xin-Fen AU - Guo XF AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Liu, Min AU - Liu M AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhou, Yu-Lin AU - Zhou YL AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Li, Zhi AU - Li Z AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhou, Li AU - Zhou L AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Wang, Zhong-Wei AU - Wang ZW AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. wzw0909@ihb.ac.cn. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. wzw0909@ihb.ac.cn. FAU - Gui, Jian-Fang AU - Gui JF AD - State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. LA - eng GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 32172974/National Natural Science Foundation of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ GR - 2018YFD0901201/National Key Research and Development Program of China/ PT - Journal Article DEP - 20230406 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 SB - IM MH - Animals MH - Female MH - Male MH - Transcriptome MH - *Catfishes/genetics MH - Gonads/metabolism MH - Ovary/metabolism MH - Gene Expression Profiling MH - *Perciformes/genetics MH - Sex Differentiation/genetics MH - Gene Expression Regulation, Developmental MH - Sex Determination Processes/genetics PMC - PMC10077648 OTO - NOTNLM OT - Gonadal transcriptome OT - Hemibagrus wyckioides OT - RNA-Seq OT - Sex determination OT - Sex differentiation COIS- The authors declare no competing interests. EDAT- 2023/04/07 06:00 MHDA- 2023/04/10 06:42 PMCR- 2023/04/06 CRDT- 2023/04/06 23:43 PHST- 2022/12/09 00:00 [received] PHST- 2023/03/21 00:00 [accepted] PHST- 2023/04/10 06:42 [medline] PHST- 2023/04/06 23:43 [entrez] PHST- 2023/04/07 06:00 [pubmed] PHST- 2023/04/06 00:00 [pmc-release] AID - 10.1186/s12864-023-09264-x [pii] AID - 9264 [pii] AID - 10.1186/s12864-023-09264-x [doi] PST - epublish SO - BMC Genomics. 2023 Apr 6;24(1):183. doi: 10.1186/s12864-023-09264-x.