PMID- 37025039
OWN - NLM
STAT- MEDLINE
DCOM- 20230509
LR  - 20230509
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 9
IP  - 5
DP  - 2023 May 8
TI  - Enhanced M2 Polarization of Oriented Macrophages on the P(VDF-TrFE) Film by 
      Coupling with Electrical Stimulation.
PG  - 2615-2624
LID - 10.1021/acsbiomaterials.2c01551 [doi]
AB  - Electrical stimulation (ES) has been considered a promising strategy in 
      regulating intracellular communication, membrane depolarization, ion transport, 
      etc. Meanwhile, cell topography, such as the alignment and elongation in 
      anisotropic orientation, also plays a critical role in triggering 
      mechanotransduction as well as the cellular fate. However, coupling of ES and 
      cell orientation to regulate the polarization of macrophages is yet to be 
      explored. In this work, we intended to explore the polarization of macrophages on 
      a poly(vinylidene fluoride-trifluoroethylene [P(VDF-TrFE)] film with intrinsic 
      microstripe roughness, which was covered on indium tin oxide planar 
      microelectrodes. We found that mouse bone marrow-derived macrophages (BMDMs) 
      cultured on a P(VDF-TrFE) film exhibited an elongated morphology aligned with the 
      microstripe crystal whisker, but their polarization behavior was not affected. 
      However, the elongated cells were susceptible to ES and upregulated their M2 
      polarization, as verified by the related expression of phenotype markers, 
      cytokines, and genes, while not affecting M1 polarization. This is due to the 
      increased expression of the M2 polarization receptor interleukin-4Ralpha on the 
      surface of elongated BMDMs, while the M1 polarization receptor toll-like receptor 
      4 was not affected. Thus, M2 polarization was singularly enhanced after 
      activation of polarization by ES. The combination of surface morphology and ES to 
      promote M2 single polarization in this work provides a new perspective for 
      regulating macrophage polarization in the field of immunotherapy.
FAU - Gu, Jiahao
AU  - Gu J
AD  - School of Materials Science and Engineering, State Key Laboratory of Silicon 
      Materials, Zhejiang University, Hangzhou 310027, China.
FAU - Wu, Chengwei
AU  - Wu C
AD  - School of Materials Science and Engineering, State Key Laboratory of Silicon 
      Materials, Zhejiang University, Hangzhou 310027, China.
FAU - He, Xuzhao
AU  - He X
AD  - School of Materials Science and Engineering, State Key Laboratory of Silicon 
      Materials, Zhejiang University, Hangzhou 310027, China.
FAU - Chen, Xiaoyi
AU  - Chen X
AD  - The Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 
      310003, China.
FAU - Dong, Lingqing
AU  - Dong L
AUID- ORCID: 0000-0002-2203-3212
AD  - The Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 
      310003, China.
FAU - Weng, Wenjian
AU  - Weng W
AUID- ORCID: 0000-0002-9373-7284
AD  - School of Materials Science and Engineering, State Key Laboratory of Silicon 
      Materials, Zhejiang University, Hangzhou 310027, China.
AD  - Center of Rehabilitation Biomedical Materials, Zhejiang University, Hangzhou 
      310027, China.
FAU - Cheng, Kui
AU  - Cheng K
AUID- ORCID: 0000-0003-4828-6450
AD  - The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 
      310003, China.
AD  - School of Materials Science and Engineering, State Key Laboratory of Silicon 
      Materials, Zhejiang University, Hangzhou 310027, China.
AD  - Center of Rehabilitation Biomedical Materials, Zhejiang University, Hangzhou 
      310027, China.
FAU - Wang, Daming
AU  - Wang D
AD  - The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 
      310003, China.
AD  - Center of Rehabilitation Biomedical Materials, Zhejiang University, Hangzhou 
      310027, China.
FAU - Chen, Zuobing
AU  - Chen Z
AD  - The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 
      310003, China.
AD  - Center of Rehabilitation Biomedical Materials, Zhejiang University, Hangzhou 
      310027, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230406
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Mechanotransduction, Cellular
MH  - *Macrophages/metabolism
MH  - Cell Differentiation
MH  - Electric Stimulation
OTO - NOTNLM
OT  - M2 polarization
OT  - P(VDF-TrFE)
OT  - electrical stimulation
OT  - macrophage
OT  - microstripe rough topography
EDAT- 2023/04/08 06:00
MHDA- 2023/05/09 06:42
CRDT- 2023/04/07 01:13
PHST- 2023/05/09 06:42 [medline]
PHST- 2023/04/08 06:00 [pubmed]
PHST- 2023/04/07 01:13 [entrez]
AID - 10.1021/acsbiomaterials.2c01551 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2023 May 8;9(5):2615-2624. doi: 
      10.1021/acsbiomaterials.2c01551. Epub 2023 Apr 6.