PMID- 37025887
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230411
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 4
DP  - 2023 Apr
TI  - AGEs-RAGE-K(Ca)3.1 pathway mediates palmitic acid-induced migration of PBMCs from 
      patients with type 2 diabetes.
PG  - e14823
LID - 10.1016/j.heliyon.2023.e14823 [doi]
LID - e14823
AB  - Type 2 diabetes mellitus (T2DM) is characterized by chronic low-grade systemic 
      inflammation. Tissue infiltration by monocyte migration contributes to the 
      pathogenesis of vascular complications in T2DM. We studied the role of 
      intermediate-conductance Ca(2+)-activated K(+) (K(Ca)3.1) channels in the 
      palmitic acid (PA)-induced migration of peripheral blood mononuclear cells 
      (PBMCs) from T2DM patients and the influence of advanced glycation endproducts 
      (AGEs). A total of 49 T2DM patients and 33 healthy subjects was recruited into 
      this study. Using flow cytometry and Western blotting analysis as well as cell 
      migration assay, we found that there was a significant decrease in frequency of T 
      lymphocytes and monocytes in CD45(+) leukocyte population. PA at 100 muM 
      stimulated migration of PBMCs from T2DM individuals, which was inhibited by the 
      specific K(Ca)3.1 channel blocker TRAM-34 (1 muM). The PBMC migration was 
      positively correlated with glycosylated hemoglobin A1 chain (HbA1c) level of T2DM 
      patients, an indicator of AGEs, and PBMCs with higher level of HbA1c showed 
      upregulated expression of toll-like receptor (TLR) 2/4 and K(Ca)3.1 channels. In 
      THP-1 cells, AGEs at 200 mug/ml increased protein expression of TLR 2/4 and 
      K(Ca)3.1 channels, and were synergistically involved in PA-induced migration 
      through receptors of AGEs (RAGE)-mediated K(Ca)3.1 upregulation. In conclusion, 
      in PBMCs of T2DM patients, AGEs promotes PA-induced migration via upregulation of 
      TLR2/4 and K(Ca)3.1 channels.
CI  - (c)2023PublishedbyElsevierLtd.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Ma, Xiao-Zhen
AU  - Ma XZ
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Zhao, Xin-Yi
AU  - Zhao XY
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Li, Jing-Jing
AU  - Li JJ
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Ma, Shan
AU  - Ma S
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
AD  - Department of Endocrinology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, 157 Fifth West Road, Xi'an, 710004, Shaanxi, China.
FAU - Pang, Zheng-Da
AU  - Pang ZD
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Xu, Jing
AU  - Xu J
AD  - Department of Endocrinology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, 157 Fifth West Road, Xi'an, 710004, Shaanxi, China.
FAU - Du, Xiao-Jun
AU  - Du XJ
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Deng, Xiu-Ling
AU  - Deng XL
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 
      710061, Shaanxi, China.
FAU - Wang, Jun-Hong
AU  - Wang JH
AD  - Department of Endocrinology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, 157 Fifth West Road, Xi'an, 710004, Shaanxi, China.
AD  - Department of Endocrinology, Gongli Hospital, Pudong New Area, Shanghai, 200135, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230324
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10070889
OTO - NOTNLM
OT  - HbA1c
OT  - Migration
OT  - Palmitic acid
OT  - Peripheral blood mononuclear cells
OT  - Type 2 diabetes mellitus
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/04/08 06:00
MHDA- 2023/04/08 06:01
PMCR- 2023/03/24
CRDT- 2023/04/07 02:35
PHST- 2022/09/03 00:00 [received]
PHST- 2023/03/08 00:00 [revised]
PHST- 2023/03/17 00:00 [accepted]
PHST- 2023/04/08 06:01 [medline]
PHST- 2023/04/07 02:35 [entrez]
PHST- 2023/04/08 06:00 [pubmed]
PHST- 2023/03/24 00:00 [pmc-release]
AID - S2405-8440(23)02030-3 [pii]
AID - e14823 [pii]
AID - 10.1016/j.heliyon.2023.e14823 [doi]
PST - epublish
SO  - Heliyon. 2023 Mar 24;9(4):e14823. doi: 10.1016/j.heliyon.2023.e14823. eCollection 
      2023 Apr.