PMID- 37027080
OWN - NLM
STAT- MEDLINE
DCOM- 20230411
LR  - 20230418
IS  - 1573-3890 (Electronic)
IS  - 1573-3882 (Linking)
VI  - 19
IP  - 4
DP  - 2023 Apr 7
TI  - Performance assessment of an equine metabolomics model for screening a range of 
      anabolic agents.
PG  - 38
LID - 10.1007/s11306-023-01985-0 [doi]
AB  - INTRODUCTION: Despite their ban, Anabolic Androgenic Steroids (AAS) are 
      considered as the most important threat for equine doping purposes. In the 
      context of controlling such practices in horse racing, metabolomics has emerged 
      as a promising alternative strategy to study the effect of a substance on 
      metabolism and to discover new relevant biomarkers of effect. Based on the 
      monitoring of 4 metabolomics derived candidate biomarkers in urine, a prediction 
      model to screen for testosterone esters abuse was previously developed. The 
      present work focuses on assessing the robustness of the associated method and 
      define its scope of application. MATERIALS AND METHODS: Several hundred urine 
      samples were selected from 14 different horses of ethically approved 
      administration studies involving various doping agents' (AAS, SARMS, beta-agonists, 
      SAID, NSAID) (328 urine samples). In addition, 553 urine samples from untreated 
      horses of doping control population were included in the study. Samples were 
      characterized with the previously described LC-HRMS/MS method, with the objective 
      of assessing both its biological and analytical robustness. RESULTS: The study 
      concluded that the measurement of the 4 biomarkers involved in the model was fit 
      for purpose. Further, the classification model confirmed its effectiveness in 
      screening for testosterone esters use; and it demonstrated its ability to screen 
      for the misuse of other anabolic agents, allowing the development of a global 
      screening tool dedicated to this class of substances. Finally, the results were 
      compared to a direct screening method targeting anabolic agents demonstrating 
      complementary performances of traditional and omics approaches in the screening 
      of anabolic agents in horses.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Cloteau, C
AU  - Cloteau C
AD  - LABERCA, ONIRIS, INRAE, 44300, Nantes, France. chloe.cloteau@oniris-nantes.fr.
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France. 
      chloe.cloteau@oniris-nantes.fr.
FAU - Dervilly, G
AU  - Dervilly G
AD  - LABERCA, ONIRIS, INRAE, 44300, Nantes, France.
FAU - Loup, B
AU  - Loup B
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Delcourt, V
AU  - Delcourt V
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Kaabia, Z
AU  - Kaabia Z
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Bagilet, F
AU  - Bagilet F
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Groseille, G
AU  - Groseille G
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Dauriac, K
AU  - Dauriac K
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Fisher, S
AU  - Fisher S
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Popot, M A
AU  - Popot MA
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Garcia, P
AU  - Garcia P
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
FAU - Le Bizec, B
AU  - Le Bizec B
AD  - LABERCA, ONIRIS, INRAE, 44300, Nantes, France.
FAU - Bailly-Chouriberry, L
AU  - Bailly-Chouriberry L
AD  - Laboratoire des Courses Hippiques (GIE-LCH), 91370, Verrieres Le Buisson, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230407
PL  - United States
TA  - Metabolomics
JT  - Metabolomics : Official journal of the Metabolomic Society
JID - 101274889
RN  - 0 (Anabolic Agents)
RN  - 0 (Steroids)
RN  - 3XMK78S47O (Testosterone)
RN  - 0 (Testosterone Congeners)
RN  - 0 (Anabolic Androgenic Steroids)
RN  - 0 (Esters)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Horses
MH  - Animals
MH  - *Anabolic Agents/urine
MH  - Tandem Mass Spectrometry/methods
MH  - Steroids
MH  - Metabolomics
MH  - Testosterone
MH  - Testosterone Congeners
MH  - Anabolic Androgenic Steroids
MH  - Esters
MH  - Biomarkers/urine
OTO - NOTNLM
OT  - Anabolic agents
OT  - Biomarkers
OT  - Doping control
OT  - Metabolomics
OT  - Screening
OT  - Steroids
EDAT- 2023/04/08 06:00
MHDA- 2023/04/11 06:42
CRDT- 2023/04/07 11:18
PHST- 2022/07/04 00:00 [received]
PHST- 2023/03/04 00:00 [accepted]
PHST- 2023/04/11 06:42 [medline]
PHST- 2023/04/07 11:18 [entrez]
PHST- 2023/04/08 06:00 [pubmed]
AID - 10.1007/s11306-023-01985-0 [pii]
AID - 10.1007/s11306-023-01985-0 [doi]
PST - epublish
SO  - Metabolomics. 2023 Apr 7;19(4):38. doi: 10.1007/s11306-023-01985-0.