PMID- 37031804
OWN - NLM
STAT- MEDLINE
DCOM- 20230517
LR  - 20230519
IS  - 1095-953X (Electronic)
IS  - 0969-9961 (Linking)
VI  - 181
DP  - 2023 Jun 1
TI  - Syntaxin 7 modulates seizure activity in epilepsy.
PG  - 106118
LID - S0969-9961(23)00132-8 [pii]
LID - 10.1016/j.nbd.2023.106118 [doi]
AB  - The exact pathogenesis of epilepsy, one of the most common and devastating 
      diseases of the nervous system, is not fully understood. Syntaxin7 (STX7) is a 
      member of the SNARE superfamily, which mediates membrane fusion events in all 
      cells. However, the role STX7 plays in epilepsy remains unclear. Therefore, this 
      study investigates the role of STX7 in epilepsy. Our study found that the 
      expression of STX7 was reduced in the epileptic brain and that overexpression of 
      STX7 decreased the susceptibility to epileptic seizures and alleviated epileptic 
      activity in a kainic acid-induced model and pentylenetetrazole-induced kindling 
      model of epilepsy, whereas the downregulation of STX7 showed opposite effects. 
      Whole-cell patch-clamp recordings showed that STX7 does not affect the intrinsic 
      excitability of neurons, but rather the excitation/inhibition ratio mediated by 
      affecting the release of presynaptic gamma-aminobutyric acid neurotransmitters. 
      Transmission electron microscopy results showed that STX7 did not affect the 
      density of inhibitory synapses but could affect the density of inhibitory 
      vesicles. Taken together, these results reveal a previously unknown function of 
      STX7 in epilepsy and suggest that STX7 may serve as a novel target for epilepsy 
      therapy.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Wu, Junhong
AU  - Wu J
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China.
FAU - Yang, Liu
AU  - Yang L
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China.
FAU - Chen, Yuanyuan
AU  - Chen Y
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China.
FAU - Li, Jiyuan
AU  - Li J
AD  - Department of Neurology, The First Hospital of Shanxi Medical University, No.85 
      Jiefang South Road, Taiyuan, Shanxi Province, China.
FAU - Yang, Min
AU  - Yang M
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China.
FAU - Zhang, Xiaogang
AU  - Zhang X
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China; Department of Neurology, Chongqing General Hospital, Chongqing Key 
      Laboratory of Neurodegenerative Diseases, No.118, Xingguang Avenue, Liangjiang 
      New Area, Chongqing 401147, China.
FAU - He, Changlong
AU  - He C
AD  - Department of Laboratory Medicine, People's Hospital of Jiulongpo District, 
      Chongqing 400016, China; Key Laboratory of Molecular Biology for Infectious 
      Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of 
      Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical 
      University, Chongqing 400016, China. Electronic address: 282702575@qq.com.
FAU - Wang, Xuefeng
AU  - Wang X
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China. Electronic address: xfyp@163.com.
FAU - Xu, Xin
AU  - Xu X
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 
      400016, China. Electronic address: xuxin86516@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230407
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Carrier Proteins)
RN  - 0 (Qa-SNARE Proteins)
SB  - IM
MH  - Humans
MH  - Carrier Proteins
MH  - *Epilepsy/metabolism
MH  - Neurons/metabolism
MH  - Qa-SNARE Proteins/metabolism
MH  - Seizures/metabolism
MH  - Animals
MH  - Mice
OTO - NOTNLM
OT  - Epilepsy
OT  - GABA
OT  - Synaptic transmission
OT  - Synaptic vesicle
OT  - Syntaxin 7
COIS- Declaration of Competing Interest None.
EDAT- 2023/04/10 06:00
MHDA- 2023/05/17 06:42
CRDT- 2023/04/09 19:24
PHST- 2023/01/31 00:00 [received]
PHST- 2023/03/18 00:00 [revised]
PHST- 2023/04/05 00:00 [accepted]
PHST- 2023/05/17 06:42 [medline]
PHST- 2023/04/10 06:00 [pubmed]
PHST- 2023/04/09 19:24 [entrez]
AID - S0969-9961(23)00132-8 [pii]
AID - 10.1016/j.nbd.2023.106118 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2023 Jun 1;181:106118. doi: 10.1016/j.nbd.2023.106118. Epub 2023 
      Apr 7.