PMID- 37031871
OWN - NLM
STAT- MEDLINE
DCOM- 20230515
LR  - 20230515
IS  - 1879-2642 (Electronic)
IS  - 0005-2736 (Linking)
VI  - 1865
IP  - 5
DP  - 2023 Jun
TI  - Incorporation of acridine orange and methylene blue in Langmuir monolayers 
      mimicking releasing nanostructures.
PG  - 184156
LID - S0005-2736(23)00038-X [pii]
LID - 10.1016/j.bbamem.2023.184156 [doi]
AB  - The efficiency of methylene blue (MB) and acridine orange (AO) for photodynamic 
      therapy (PDT) is increased if encapsulated in liposomes. In this paper we 
      determine the molecular-level interactions between MB or AO and mixed monolayers 
      of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 
      1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) and cholesterol 
      (CHOL) using surface pressure isotherms and polarization-modulated infrared 
      reflection absorption spectroscopy (PM-IRRAS). To increase liposome stability, 
      the effects from adding the surfactants Span(R) 80 and sodium cholate were also 
      studied. Both MB and AO induce an expansion in the mixed monolayer, but this 
      expansion is less significant in the presence of either Span(R) 80 or sodium 
      cholate. The action of AO and MB occurred via coupling with phosphate groups of 
      DPPC or DPPG. However, the levels of chain ordering and hydration of carbonyl and 
      phosphate in headgroups depended on the photosensitizer and on the presence of 
      Span(R) 80 or sodium cholate. From the PM-IRRAS spectra, we inferred that 
      incorporation of MB and AO increased hydration of the monolayer headgroup, except 
      for the case of the monolayer containing sodium cholate. This variability in 
      behaviour offers an opportunity to tune the incorporation of AO and MB into 
      liposomes which could be exploited in the release necessary for PDT.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Pivetta, Thais P
AU  - Pivetta TP
AD  - CEFITEC, Department of Physics, NOVA School of Science and Technology, 
      Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal; Laboratory of 
      Instrumentation, Biomedical Engineering and Radiation Physics (LIBPhys-UNL), 
      Department of Physics, NOVA School of Science and Technology, Universidade NOVA 
      de Lisboa, 2829-516 Caparica, Portugal.
FAU - Jochelavicius, Karen
AU  - Jochelavicius K
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, Brazil.
FAU - Wrobel, Ellen C
AU  - Wrobel EC
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, Brazil.
FAU - Balogh, Debora T
AU  - Balogh DT
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, Brazil.
FAU - Oliveira, Osvaldo N Jr
AU  - Oliveira ON Jr
AD  - Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, Brazil.
FAU - Ribeiro, Paulo A
AU  - Ribeiro PA
AD  - Laboratory of Instrumentation, Biomedical Engineering and Radiation Physics 
      (LIBPhys-UNL), Department of Physics, NOVA School of Science and Technology, 
      Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
FAU - Raposo, Maria
AU  - Raposo M
AD  - Laboratory of Instrumentation, Biomedical Engineering and Radiation Physics 
      (LIBPhys-UNL), Department of Physics, NOVA School of Science and Technology, 
      Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: 
      mfr@fct.unl.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230407
PL  - Netherlands
TA  - Biochim Biophys Acta Biomembr
JT  - Biochimica et biophysica acta. Biomembranes
JID - 101731713
RN  - T42P99266K (Methylene Blue)
RN  - F30N4O6XVV (Acridine Orange)
RN  - 0 (Liposomes)
RN  - NU3Y4CCH8Z (Sodium Cholate)
SB  - IM
MH  - *Methylene Blue
MH  - *Acridine Orange
MH  - Liposomes
MH  - Sodium Cholate
MH  - Spectrophotometry, Infrared
OTO - NOTNLM
OT  - Acridine orange
OT  - Langmuir
OT  - Methylene blue
OT  - Mixed monolayers
OT  - Photosensitizer
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/04/10 06:00
MHDA- 2023/05/15 06:42
CRDT- 2023/04/09 19:25
PHST- 2022/09/07 00:00 [received]
PHST- 2023/03/15 00:00 [revised]
PHST- 2023/03/22 00:00 [accepted]
PHST- 2023/05/15 06:42 [medline]
PHST- 2023/04/10 06:00 [pubmed]
PHST- 2023/04/09 19:25 [entrez]
AID - S0005-2736(23)00038-X [pii]
AID - 10.1016/j.bbamem.2023.184156 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Biomembr. 2023 Jun;1865(5):184156. doi: 
      10.1016/j.bbamem.2023.184156. Epub 2023 Apr 7.