PMID- 37034711
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231106
DP  - 2023 Mar 29
TI  - Cultured Mesenchymal Cells from Nasal Turbinate as a Cellular Model of the 
      Neurodevelopmental Component of Schizophrenia Etiology.
LID - 2023.03.28.534295 [pii]
LID - 10.1101/2023.03.28.534295 [doi]
AB  - Study of the neurodevelopmental molecular mechanisms of schizophrenia requires 
      the development of adequate biological models such as patient-derived cells and 
      their derivatives. We previously used cell lines with neural progenitor 
      properties (CNON) derived from superior or middle turbinates of patients with 
      schizophrenia and control groups to study gene expression specific to 
      schizophrenia. In this study, we compared single cell-RNA seq data from two CNON 
      cell lines, one derived from an individual with schizophrenia (SCZ) and the other 
      from a control group, with two biopsy samples from the middle turbinate (MT), 
      also from an individual with SCZ and a control. In addition, we compared our data 
      with previously published data from olfactory neuroepithelium (1). Our data 
      demonstrated that CNON originated from a single cell type which is present both 
      in middle turbinate and olfactory neuroepithelium. CNON express multiple markers 
      of mesenchymal cells. In order to define relatedness of CNON to the developing 
      human brain, we also compared CNON datasets with scRNA-seq data of embryonic 
      brain (2) and found that the expression profile of CNON very closely matched one 
      of the cell types in the embryonic brain. Finally, we evaluated differences 
      between SCZ and control samples to assess usability and potential benefits of 
      using single cell RNA-seq of CNON to study etiology of schizophrenia.
FAU - Tung, Victoria Sook Keng
AU  - Tung VSK
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY, USA.
FAU - Mathews, Fasil
AU  - Mathews F
AD  - Department of Otolaryngology, State University of New York at Downstate, 
      Brooklyn, NY, USA.
FAU - Boruk, Marina
AU  - Boruk M
AD  - Department of Otolaryngology, State University of New York at Downstate, 
      Brooklyn, NY, USA.
FAU - Suppa, Gabrielle
AU  - Suppa G
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY, USA.
FAU - Foronjy, Robert
AU  - Foronjy R
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY, USA.
FAU - Pato, Michele
AU  - Pato M
AD  - Department of Psychiatry, Rutgers University.
FAU - Pato, Carlos
AU  - Pato C
AD  - Department of Psychiatry, Rutgers University.
FAU - Knowles, James A
AU  - Knowles JA
AD  - Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
FAU - Evgrafov, Oleg V
AU  - Evgrafov OV
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY, USA.
LA  - eng
GR  - R01 MH086874/MH/NIMH NIH HHS/United States
GR  - R01 MH104964/MH/NIMH NIH HHS/United States
GR  - R01 MH123451/MH/NIMH NIH HHS/United States
PT  - Preprint
DEP - 20230329
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
UIN - Int J Mol Sci. 2023 Oct 19;24(20):. PMID: 37895019
PMC - PMC10081251
OTO - NOTNLM
OT  - mesenchymal cells
OT  - middle turbinate
OT  - neural progenitors
OT  - scRNA-seq
OT  - schizophrenia
COIS- Conflict of Interest The authors declare that the research was conducted in the 
      absence of any commercial or financial relationships that could be construed as a 
      potential conflict of interest.
EDAT- 2023/04/11 06:00
MHDA- 2023/04/11 06:01
PMCR- 2023/04/07
CRDT- 2023/04/10 04:12
PHST- 2023/04/11 06:00 [pubmed]
PHST- 2023/04/11 06:01 [medline]
PHST- 2023/04/10 04:12 [entrez]
PHST- 2023/04/07 00:00 [pmc-release]
AID - 2023.03.28.534295 [pii]
AID - 10.1101/2023.03.28.534295 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Mar 29:2023.03.28.534295. doi: 
      10.1101/2023.03.28.534295.