PMID- 37037782
OWN - NLM
STAT- MEDLINE
DCOM- 20230414
LR  - 20230414
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 76
IP  - 4
DP  - 2023 Apr 3
TI  - The correlation between denaturation of glyceraldehyde-3-phosphate dehydrogenase 
      and inactivation of Saccharomyces pastorianus cells by pressurized carbon dioxide 
      microbubbles.
LID - ovad043 [pii]
LID - 10.1093/lambio/ovad043 [doi]
AB  - For the purpose of clarifying the relationship between pasteurization and 
      inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Saccharomyces 
      pastorianus cells induced by pressurized carbon dioxide microbubbles (CO2MB) 
      treatment, a storage test of S. pastorianus cells after CO2MB treatment was 
      conducted to ascertain their recovery, and the treatment condition in the 
      inactivation of GAPDH in S. pastorianus cells by CO2MB was investigated. Each 
      population of S. pastorianus for 48, 96, and 144 h at 25 degrees C was decreased 
      significantly by CO2MB treatment at 35 degrees C for 3 min (MB35-3 and MB35-5) or at 40 degrees C 
      and 45 degrees C for 1 and 3 min (MB40-1, MB40-3, and MB45-1). In the storage test, 
      recovery of treated cells was not observed after storage for 144 h at 25 degrees C. The 
      denaturation of GAPDH in the S. pastorianus cells caused by the same treatment as 
      the storage test was detected by using sodium dodecyl sulphate polyacrylamide gel 
      electrophoresis. While the activities at MB35-1, MB35-3, and MB40-1 were 
      significantly higher than those at non-treatment, and those at MB35-5, MB40-3, 
      and MB45-1 were lower. Therefore, GAPDH denaturation, but not the activity, was 
      associated with the inactivation of S. pastorianus cells.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Applied 
      Microbiology International.
FAU - Takahashi, Konomi
AU  - Takahashi K
AD  - Faculty of Applied Life Science, Nippon Veterinary and Life Science University, 
      Musashino 180-8602, Tokyo, Japan.
FAU - Kobayashi, Fumiyuki
AU  - Kobayashi F
AD  - Faculty of Applied Life Science, Nippon Veterinary and Life Science University, 
      Musashino 180-8602, Tokyo, Japan.
FAU - Narai-Kanayama, Asako
AU  - Narai-Kanayama A
AD  - Faculty of Applied Life Science, Nippon Veterinary and Life Science University, 
      Musashino 180-8602, Tokyo, Japan.
FAU - Odake, Sachiko
AU  - Odake S
AD  - Faculty of Applied Life Science, Nippon Veterinary and Life Science University, 
      Musashino 180-8602, Tokyo, Japan.
LA  - eng
GR  - 20K05896/JSPS/
PT  - Journal Article
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - Saccharomyces pastorianus
SB  - IM
MH  - *Carbon Dioxide/pharmacology
MH  - Microbubbles
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases
MH  - *Saccharomyces
OTO - NOTNLM
OT  - Saccharomyces pastorianus
OT  - denaturation
OT  - glyceraldehyde-3-phosphate dehydrogenase
OT  - inactivation
OT  - pressurized carbon dioxide microbubbles
EDAT- 2023/04/11 06:00
MHDA- 2023/04/14 06:41
CRDT- 2023/04/10 22:03
PHST- 2022/12/06 00:00 [received]
PHST- 2023/03/08 00:00 [revised]
PHST- 2023/04/07 00:00 [accepted]
PHST- 2023/04/14 06:41 [medline]
PHST- 2023/04/11 06:00 [pubmed]
PHST- 2023/04/10 22:03 [entrez]
AID - 7112098 [pii]
AID - 10.1093/lambio/ovad043 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2023 Apr 3;76(4):ovad043. doi: 10.1093/lambio/ovad043.