PMID- 37038227
OWN - NLM
STAT- MEDLINE
DCOM- 20230412
LR  - 20230413
IS  - 1757-2215 (Electronic)
IS  - 1757-2215 (Linking)
VI  - 16
IP  - 1
DP  - 2023 Apr 11
TI  - Toxic effect window of ovarian development in female offspring mice induced by 
      prenatal prednisone exposure with different doses and time.
PG  - 71
LID - 10.1186/s13048-023-01148-8 [doi]
LID - 71
AB  - BACKGROUND: Prednisone is one of the most used synthetic glucocorticoids during 
      pregnancy. Epidemiological investigations suggested that prenatal prednisone 
      therapy could affect fetal development, but systematic studies on its effects on 
      ovarian development and the "toxic effect window" remained scarce. METHODS: In 
      this study, by simulating clinical application characteristics, Kunming mice were 
      given prednisone by oral gavage with different doses (0.25 or 1.0 mg/kg.d) or at 
      different time gestational days (GD) (GD0-9, GD10-18, or GD0-18). Blood and 
      ovaries of fetal mice were collected on GD18, and the serum estradiol level and 
      the related function indexes of ovarian granulosa cells and oocytes were 
      detected. RESULTS: Compared with the control group, prenatal prednisone exposure 
      (PPE) induced pathological injury and enhanced cell proliferation in fetal mice 
      ovary. Furthermore, the expression of steroid synthesis functional genes in 
      pre-granulosa cells, the oocyte function markers, and developmentally related 
      genes was enhanced with different doses or at different time of PPE. The Hippo 
      signaling was activated in the fetal ovary of PPE groups. The above changes were 
      most significant in the low or high-dose and full-term PPE groups. CONCLUSION: 
      PPE caused various cell developmental toxicity in the fetal ovary, especially in 
      the low or high-dose, full-term exposure groups. The potential mechanism might be 
      related to the activation of the Hippo signaling pathway.
CI  - (c) 2023. The Author(s).
FAU - Huang, Jing
AU  - Huang J
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      Wuhan, 430071, China.
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Zhongnan Hospital of 
      Wuhan University, Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Wu, Tiancheng
AU  - Wu T
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Li, Yating
AU  - Li Y
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Zhang, Yuanzhen
AU  - Zhang Y
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Yu, Xingjiang
AU  - Yu X
AD  - Department of Histology and Embryology, School of Basic Medicine, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, 430030, China. 
      yuxingjiang@hust.edu.cn.
FAU - Xu, Dan
AU  - Xu D
AD  - Department of Pharmacy, School of Pharmaceutical Sciences, Zhongnan Hospital of 
      Wuhan University, Wuhan University, Wuhan, 430071, China. 
      xuyidan70188@whu.edu.cn.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China. xuyidan70188@whu.edu.cn.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, 
      Wuhan, 430071, China. wanghui19@whu.edu.cn.
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, 430071, China. wanghui19@whu.edu.cn.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China. wanghui19@whu.edu.cn.
LA  - eng
GR  - No. 2020YFA0803900/National Key Research and Development Program of China/
GR  - No. 81903729, 82030111/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230411
PL  - England
TA  - J Ovarian Res
JT  - Journal of ovarian research
JID - 101474849
RN  - 4TI98Z838E (Estradiol)
RN  - VB0R961HZT (Prednisone)
RN  - Kunming mice
SB  - IM
MH  - Mice
MH  - Pregnancy
MH  - Female
MH  - Animals
MH  - *Estradiol
MH  - Prednisone/metabolism/pharmacology
MH  - *Ovary
MH  - Oocytes/metabolism
PMC - PMC10088227
OTO - NOTNLM
OT  - Oocyte
OT  - Ovarian developmental toxicity
OT  - Pre-granulosa cell
OT  - Prednisone
OT  - Prenatal exposure
COIS- On behalf of all authors, the corresponding author states that there is no 
      conflict of interest.
EDAT- 2023/04/11 06:00
MHDA- 2023/04/12 06:41
PMCR- 2023/04/11
CRDT- 2023/04/10 23:50
PHST- 2022/12/14 00:00 [received]
PHST- 2023/03/29 00:00 [accepted]
PHST- 2023/04/12 06:41 [medline]
PHST- 2023/04/10 23:50 [entrez]
PHST- 2023/04/11 06:00 [pubmed]
PHST- 2023/04/11 00:00 [pmc-release]
AID - 10.1186/s13048-023-01148-8 [pii]
AID - 1148 [pii]
AID - 10.1186/s13048-023-01148-8 [doi]
PST - epublish
SO  - J Ovarian Res. 2023 Apr 11;16(1):71. doi: 10.1186/s13048-023-01148-8.