PMID- 37043376
OWN - NLM
STAT- MEDLINE
DCOM- 20230414
LR  - 20230414
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 72
IP  - 4
DP  - 2023 Apr
TI  - The CpxRA two-component system represses gene expression of the heat-labile toxin 
      of enterotoxigenic Escherichia coli.
LID - 10.1099/jmm.0.001682 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) strains produce at least one of two types 
      of enterotoxins: the heat-labile (LT) and heat-stable (ST) toxins, which are 
      responsible for the watery secretory diarrhoea that is a hallmark of the human 
      ETEC infection. One regulatory system that controls the transcription of 
      virulence genes in pathogenic bacteria is the CpxRA two-component system (TCS). 
      We reported that the eltAB bicistronic operon, which encodes for the A and B 
      subunits of LT, was repressed for the CpxRA TCS by direct binding of CpxR-P from 
      -12 to +6 bp with respect to the transcription start site of eltAB. Moreover, the 
      Cpx-response activation down-regulated the transcription of eltAB genes, and this 
      negative effect was CpxRA-dependent. Our data show that CpxRA TCS is a negative 
      regulator of the LT, one of the main virulence determinants of ETEC.
FAU - Rodriguez-Valverde, Diana
AU  - Rodriguez-Valverde D
AD  - Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, 
      Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del 
      Seguro Social, Mexico City, Mexico.
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional, Mexico City, Mexico.
AD  - Facultad de Ciencias Quimicas, Universidad Autonoma de Chihuahua, Chihuahua, 
      Mexico.
FAU - Leon-Montes, Nancy
AU  - Leon-Montes N
AD  - Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, 
      Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del 
      Seguro Social, Mexico City, Mexico.
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional, Mexico City, Mexico.
FAU - Siqueiros-Cendon, Tania
AU  - Siqueiros-Cendon T
AD  - Facultad de Ciencias Quimicas, Universidad Autonoma de Chihuahua, Chihuahua, 
      Mexico.
FAU - Rivera-Gutierrez, Sandra
AU  - Rivera-Gutierrez S
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional, Mexico City, Mexico.
FAU - Ares, Miguel A
AU  - Ares MA
AD  - Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, 
      Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del 
      Seguro Social, Mexico City, Mexico.
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional, Mexico City, Mexico.
FAU - De la Cruz, Miguel A
AU  - De la Cruz MA
AD  - Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, 
      Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del 
      Seguro Social, Mexico City, Mexico.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Bacterial Toxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Humans
MH  - *Enterotoxigenic Escherichia coli/genetics/metabolism
MH  - *Bacterial Toxins/genetics/metabolism
MH  - Hot Temperature
MH  - *Escherichia coli Proteins/genetics/metabolism
MH  - Enterotoxins/genetics/metabolism
MH  - *Escherichia coli Infections/microbiology
MH  - Diarrhea/microbiology
MH  - Gene Expression
OTO - NOTNLM
OT  - CpxRA
OT  - ETEC
OT  - eltAB
OT  - heat-labile toxin
EDAT- 2023/04/13 06:00
MHDA- 2023/04/14 06:41
CRDT- 2023/04/12 12:53
PHST- 2023/04/14 06:41 [medline]
PHST- 2023/04/12 12:53 [entrez]
PHST- 2023/04/13 06:00 [pubmed]
AID - 10.1099/jmm.0.001682 [doi]
PST - ppublish
SO  - J Med Microbiol. 2023 Apr;72(4). doi: 10.1099/jmm.0.001682.