PMID- 37045933
OWN - NLM
STAT- MEDLINE
DCOM- 20230414
LR  - 20230426
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Apr 12
TI  - Establishment of an artificial particulate matter-induced lung disease model 
      through analyzing pathological changes and transcriptomic profiles in mice.
PG  - 5955
LID - 10.1038/s41598-023-29919-9 [doi]
LID - 5955
AB  - Particulate matter (PM), an environmental risk factor, is linked with health 
      risks such as respiratory diseases. This study aimed to establish an animal model 
      of PM-induced lung injury with artificial PM (APM) and identify the potential of 
      APM for toxicological research. APM was generated from graphite at 600  degrees C and 
      combined with ethylene. We analyzed diesel exhaust particulate (DEP) and APM 
      compositions and compared toxicity and transcriptomic profiling in lungs 
      according to the exposure. For the animal study, C57BL/6 male mice were 
      intratracheally administered vehicle, DEP, or APM. DEP or APM increased relative 
      lung weight, inflammatory cell numbers, and inflammatory protein levels compared 
      with the vehicle control. Histological assessments showed an increase in 
      particle-pigment alveolar macrophages and slight inflammation in the lungs of DEP 
      and APM mice. In the only APM group, granulomatous inflammation, pulmonary 
      fibrosis, and mucous hyperplasia were observed in the lungs of some individuals. 
      This is the first study to compare pulmonary toxicity between DEP and APM in an 
      animal model. Our results suggest that the APM-treated animal model may 
      contribute to understanding the harmful effects of PM in toxicological studies 
      showing that APM can induce various lung diseases according to different doses of 
      APM.
CI  - (c) 2023. The Author(s).
FAU - Kim, Dong Im
AU  - Kim DI
AD  - Jeonbuk Department of Inhalation Research, Inhalation Toxicology Center for 
      Airborne Risk Factor, Korea Institute of Toxicology, 30 Baekhak1-Gil, Jeongeup, 
      Jeollabuk-Do, 56212, Republic of Korea.
FAU - Song, Mi-Kyung
AU  - Song MK
AD  - Jeonbuk Department of Inhalation Research, Inhalation Toxicology Center for 
      Airborne Risk Factor, Korea Institute of Toxicology, 30 Baekhak1-Gil, Jeongeup, 
      Jeollabuk-Do, 56212, Republic of Korea.
AD  - Department of Human and Environmental Toxicology, University of Science & 
      Technology, Daejeon, 34113, Republic of Korea.
FAU - Yuk, Ji Eun
AU  - Yuk JE
AD  - Jeonbuk Department of Inhalation Research, Inhalation Toxicology Center for 
      Airborne Risk Factor, Korea Institute of Toxicology, 30 Baekhak1-Gil, Jeongeup, 
      Jeollabuk-Do, 56212, Republic of Korea.
FAU - Seo, Hyeon Jin
AU  - Seo HJ
AD  - Jeonbuk Department of Inhalation Research, Inhalation Toxicology Center for 
      Airborne Risk Factor, Korea Institute of Toxicology, 30 Baekhak1-Gil, Jeongeup, 
      Jeollabuk-Do, 56212, Republic of Korea.
FAU - Lee, Kyuhong
AU  - Lee K
AD  - Jeonbuk Department of Inhalation Research, Inhalation Toxicology Center for 
      Airborne Risk Factor, Korea Institute of Toxicology, 30 Baekhak1-Gil, Jeongeup, 
      Jeollabuk-Do, 56212, Republic of Korea. khlee@kitox.re.kr.
AD  - Department of Human and Environmental Toxicology, University of Science & 
      Technology, Daejeon, 34113, Republic of Korea. khlee@kitox.re.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230412
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Particulate Matter)
RN  - 0 (Vehicle Emissions)
SB  - IM
MH  - Mice
MH  - Male
MH  - Animals
MH  - *Particulate Matter/toxicity/metabolism
MH  - Transcriptome
MH  - Vehicle Emissions/toxicity
MH  - Mice, Inbred C57BL
MH  - Lung/pathology
MH  - *Lung Diseases/chemically induced/genetics
PMC - PMC10097713
COIS- The authors declare no competing interests.
EDAT- 2023/04/13 06:00
MHDA- 2023/04/14 06:42
PMCR- 2023/04/12
CRDT- 2023/04/12 23:21
PHST- 2022/11/03 00:00 [received]
PHST- 2023/02/13 00:00 [accepted]
PHST- 2023/04/14 06:42 [medline]
PHST- 2023/04/12 23:21 [entrez]
PHST- 2023/04/13 06:00 [pubmed]
PHST- 2023/04/12 00:00 [pmc-release]
AID - 10.1038/s41598-023-29919-9 [pii]
AID - 29919 [pii]
AID - 10.1038/s41598-023-29919-9 [doi]
PST - epublish
SO  - Sci Rep. 2023 Apr 12;13(1):5955. doi: 10.1038/s41598-023-29919-9.