PMID- 37045944
OWN - NLM
STAT- MEDLINE
DCOM- 20230509
LR  - 20230509
IS  - 1476-5470 (Electronic)
IS  - 1466-4879 (Print)
IS  - 1466-4879 (Linking)
VI  - 24
IP  - 2
DP  - 2023 Apr
TI  - Long non-coding RNA maternally expressed gene 3, miR-125a-5p, CXCL13, and NF-kB 
      in patients with immune thrombocytopenia.
PG  - 108-115
LID - 10.1038/s41435-023-00200-3 [doi]
AB  - The main aim of this study was to assess the expression level of circulating long 
      non-coding RNA maternally expressed gene 3 (lncRNA-MEG3), microRNA (miR-125a-5P), 
      the chemokine C-X-C motif ligand13 (CXCL13), and the nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kB) in immune 
      thrombocytopenia (ITP) cases and to study its relation to the disease severity 
      and treatment response. This case-control study included 45 patients newly 
      diagnosed as ITP and 45 healthy subjects. We assessed complete blood count, 
      antinuclear antibodies, hepatitis B and C virus serology, lncRNA-MEG3, 
      miR-125a-5P, and CXCL13 expression in serum by real-time PCR and NF-kb protein by 
      ELISA. In ITP patients compared to control, lncRNA-MEG3 was significantly 
      increased, and miRNA-125a-5P was decreased, and this was associated with higher 
      CXCL13 and NF-kB levels (P < 0.001, for all).There was a significant negative 
      correlation between platelet count and lncRNA-MEG3, CXCL13, and NF-kb, while a 
      positive correlation with miR-125a-5p in ITP patients. Patients who responded to 
      steroids had significantly higher miR-125a-5p (P = 0.016) and significantly lower 
      lncRNA-MEG3 (P < 0.001), CXCL13 (P = 0.005), and NF-kb (p = 0.002). Based on the 
      ROC curves, lncRNA-MEG3 displayed the highest area under the curve (AUC) in the 
      identification of organ bleeding (AUC = 0.805), the response to steroids 
      (AUC = 0.853), and the need for splenectomy (AUC = 0.75).
CI  - (c) 2023. The Author(s).
FAU - Naguib, Mervat
AU  - Naguib M
AUID- ORCID: 0000-0002-9345-1542
AD  - Internal Medicine Department, Faculty of Medicine Kasr Al-Ainy Hospital, Cairo 
      University, Cairo, Egypt. mervat.naguib@cu.edu.eg.
FAU - El Sawy, Shereen
AU  - El Sawy S
AD  - Internal Medicine Department, Faculty of Medicine Kasr Al-Ainy Hospital, Cairo 
      University, Cairo, Egypt.
FAU - Rashed, Laila
AU  - Rashed L
AD  - Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
FAU - AlHelf, Maha
AU  - AlHelf M
AD  - Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
AD  - Biotechnology School, Nile University, Giza, Egypt.
FAU - Abdelgwad, Marwa
AU  - Abdelgwad M
AD  - Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20230412
PL  - England
TA  - Genes Immun
JT  - Genes and immunity
JID - 100953417
RN  - 0 (Chemokine CXCL13)
RN  - 0 (CXCL13 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MEG3 non-coding RNA, human)
RN  - 0 (MIRN125 microRNA, human)
SB  - IM
MH  - Humans
MH  - Case-Control Studies
MH  - *Chemokine CXCL13/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - NF-kappa B/genetics
MH  - *Purpura, Thrombocytopenic, Idiopathic/genetics
MH  - *RNA, Long Noncoding/genetics
PMC - PMC10110462
COIS- The authors declare no competing interests.
EDAT- 2023/04/13 06:00
MHDA- 2023/04/19 06:42
PMCR- 2023/04/12
CRDT- 2023/04/12 23:22
PHST- 2022/10/06 00:00 [received]
PHST- 2023/02/02 00:00 [accepted]
PHST- 2023/01/27 00:00 [revised]
PHST- 2023/04/19 06:42 [medline]
PHST- 2023/04/13 06:00 [pubmed]
PHST- 2023/04/12 23:22 [entrez]
PHST- 2023/04/12 00:00 [pmc-release]
AID - 10.1038/s41435-023-00200-3 [pii]
AID - 200 [pii]
AID - 10.1038/s41435-023-00200-3 [doi]
PST - ppublish
SO  - Genes Immun. 2023 Apr;24(2):108-115. doi: 10.1038/s41435-023-00200-3. Epub 2023 
      Apr 12.