PMID- 37046194
OWN - NLM
STAT- Publisher
LR  - 20230412
IS  - 1875-5402 (Electronic)
IS  - 1386-2073 (Linking)
DP  - 2023 Apr 12
TI  - Linarin Ameliorates Diabetic Liver Injury by Alleviating Oxidative Stress and 
      Inflammation through the Inhibition of AKR1B1.
LID - 10.2174/1386207326666230412084201 [doi]
AB  - AIMS: The study aims to find a new functional additive for diabetic liver injury. 
      BACKGROUND: It is well-established that type 2 diabetes mellitus (T2DM) is a 
      metabolic disease with multiple complications and places a significant health and 
      economic burden on modern society. Linarin is a natural flavonoid isolated from 
      Asteraceae and Lamiaceae, which has beneficial effects in preventing and treating 
      metabolic diseases such as nonalcoholic steatohepatitis and diabetes. OBJECTIVE: 
      We aimed to investigate the pharmacological effect and underlying mechanism of 
      linarin on T2DM-associated liver injury in vivo and in vitro. METHODS: Using a 
      high-glucose and high-palmitic acid-induced hepatocyte injury model and a type 2 
      diabetic rat model. Following linarin treatment, serum biochemical parameters, 
      liver histology, and lipid profiles of rats were examined. Oxidative stress index 
      and inflammatory response were detected in vivo and in vitro. The expression 
      level of AKR1B1 in rat liver tissues and in vitro cells was detected by western 
      blot and by real-time fluorescent quantitative PCR. RESULTS: The present study 
      found that linarin could prevent oxidative stress and inflammation. In 
      high-fat-fed diabetic rats, linarin administration (15, 30, and 60 mg/kg/day) 
      reduced hepatic lipid accumulation, oxidative stress, and inflammation. Linarin 
      (20 muM) significantly alleviated oxidative stress, inflammation, and apoptosis 
      induced by high glucose combined with palmitic acid in LX-2 cells. Western 
      blotting and overexpression experiments showed that these effects were related to 
      AKR1B1 inhibition in vivo and in vitro. CONCLUSION: This study indicated that 
      linarin could protect against liver injury in T2DM by alleviating oxidative 
      stress and inflammation mediated by AKR1B1 and may be a promising additive for 
      diabetic liver injury therapy.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Wang, Tong
AU  - Wang T
AD  - Department of Integrative Medicine, Xinqiao Hospital, Army Medical University, 
      Chongqing 400037, China.
FAU - Shan, Meng-Ya
AU  - Shan MY
AD  - Department of Integrative Medicine, Xinqiao Hospital, Army Medical University, 
      Chongqing 400037, China.
FAU - Tang, Cui-Yao
AU  - Tang CY
AD  - Department of Integrative Medicine, Xinqiao Hospital, Army Medical University, 
      Chongqing 400037, China.
FAU - Cheng, Mao-Yuan
AU  - Cheng MY
AD  - Department of Integrative Medicine, Xinqiao Hospital, Army Medical University, 
      Chongqing 400037, China.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Biochemistry and Molecular Biology, Army Medical University, 
      Chongqing 400038, China.
FAU - Yan, Jun
AU  - Yan J
AD  - Department 1, Research Institute of Surgery & Daping Hospital, Army Medical 
      Center of Chinese People's Liberation Army, Army Medical University, Chongqing 
      400042, China.
FAU - Xu, Zi-Hui
AU  - Xu ZH
AD  - Department of Integrative Medicine, Xinqiao Hospital, Army Medical University, 
      Chongqing 400037, China.
LA  - eng
PT  - Journal Article
DEP - 20230412
PL  - United Arab Emirates
TA  - Comb Chem High Throughput Screen
JT  - Combinatorial chemistry & high throughput screening
JID - 9810948
SB  - IM
OTO - NOTNLM
OT  - AKR1B1.
OT  - Diabetic liver injury
OT  - Inflammation
OT  - Linarin
OT  - Oxidative stress
OT  - Type 2 diabetes mellitus
EDAT- 2023/04/13 06:00
MHDA- 2023/04/13 06:00
CRDT- 2023/04/12 23:43
PHST- 2022/07/18 00:00 [received]
PHST- 2023/02/08 00:00 [revised]
PHST- 2023/02/23 00:00 [accepted]
PHST- 2023/04/12 23:43 [entrez]
PHST- 2023/04/13 06:00 [pubmed]
PHST- 2023/04/13 06:00 [medline]
AID - CCHTS-EPUB-130872 [pii]
AID - 10.2174/1386207326666230412084201 [doi]
PST - aheadofprint
SO  - Comb Chem High Throughput Screen. 2023 Apr 12. doi: 
      10.2174/1386207326666230412084201.