PMID- 37048096
OWN - NLM
STAT- MEDLINE
DCOM- 20230414
LR  - 20230427
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 7
DP  - 2023 Mar 27
TI  - Isolated Peptide from Spider Venom Modulates Dendritic Cells In Vitro: A Possible 
      Application in Oncoimmunotherapy for Glioblastoma.
LID - 10.3390/cells12071023 [doi]
LID - 1023
AB  - Dendritic cells (DCs) vaccine is a potential tool for oncoimmunotherapy. However, 
      it is known that this therapeutic strategy has failed in solid tumors, making the 
      development of immunoadjuvants highly relevant. Recently, we demonstrated that 
      Phoneutria nigriventer spider venom (PnV) components are cytotoxic to 
      glioblastoma (GB) and activate macrophages for an antitumor profile. However, the 
      effects of these molecules on the adaptive immune response have not yet been 
      evaluated. This work aimed to test PnV and its purified fractions in DCs in 
      vitro. For this purpose, bone marrow precursors were collected from male C57BL6 
      mice, differentiated into DCs and treated with venom or PnV-isolated fractions 
      (F1-molecules < 3 kDa, F2-3 to 10 kDa and F3->10 kDa), with or without 
      costimulation with human GB lysate. The results showed that mainly F1 was able to 
      activate DCs, increasing the activation-dependent surface marker (CD86) and 
      cytokine release (IL-1beta, TNF-alpha), in addition to inducing a typical morphology of 
      mature DCs. From the F1 purification, a molecule named LW9 was the most 
      effective, and mass spectrometry showed it to be a peptide. The present findings 
      suggest that this molecule could be an immunoadjuvant with possible application 
      in DC vaccines for the treatment of GB.
FAU - de Mato, Felipe Cezar
AU  - de Mato FC
AD  - Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), 
      Campinas 13083-871, SP, Brazil.
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - Barreto, Natalia
AU  - Barreto N
AUID- ORCID: 0000-0001-6150-3582
AD  - Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), 
      Campinas 13083-871, SP, Brazil.
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - Cordeiro, Gabriel
AU  - Cordeiro G
AD  - Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), 
      Campinas 13083-871, SP, Brazil.
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - Munhoz, Jaqueline
AU  - Munhoz J
AUID- ORCID: 0000-0002-3112-3033
AD  - Department of Agricultural, Food and Nutritional Sciences (AFNS), University of 
      Alberta, Edmonton, AB T6G 2R3, Canada.
FAU - Bonfanti, Amanda Pires
AU  - Bonfanti AP
AUID- ORCID: 0000-0003-0896-0105
AD  - Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), 
      Campinas 13083-871, SP, Brazil.
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - da Rocha-E-Silva, Thomaz A A
AU  - da Rocha-E-Silva TAA
AD  - Department of Physiological Sciences, Faculdade Israelita de Ciencias da Saude 
      Albert Einstein, Sao Paulo 05653-120, SP, Brazil.
FAU - Sutti, Rafael
AU  - Sutti R
AUID- ORCID: 0000-0002-5648-2446
AD  - Valer Laboratorios Eireli, Sao Paulo 13347-633, SP, Brazil.
FAU - Cruz, Priscilla B M
AU  - Cruz PBM
AD  - Department of Physiological Sciences, Faculdade Israelita de Ciencias da Saude 
      Albert Einstein, Sao Paulo 05653-120, SP, Brazil.
FAU - Sanches, Livia R
AU  - Sanches LR
AD  - Department of Physiological Sciences, Faculdade Israelita de Ciencias da Saude 
      Albert Einstein, Sao Paulo 05653-120, SP, Brazil.
FAU - Bombeiro, Andre Luis
AU  - Bombeiro AL
AUID- ORCID: 0000-0003-2303-8127
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - Chalbatani, Ghanbar Mahmoodi
AU  - Chalbatani GM
AUID- ORCID: 0000-0003-0738-4708
AD  - Department of Immunology, Mayo Clinic, Scottsdale, AZ 85259, USA.
FAU - Verinaud, Liana
AU  - Verinaud L
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
FAU - Raposo, Catarina
AU  - Raposo C
AD  - Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), 
      Campinas 13083-871, SP, Brazil.
AD  - Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 
      Campinas 13083-862, SP, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230327
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Spider Venoms)
SB  - IM
MH  - Mice
MH  - Male
MH  - Humans
MH  - Animals
MH  - *Glioblastoma/therapy
MH  - *Spider Venoms/pharmacology
MH  - Mice, Inbred C57BL
MH  - Cell Differentiation
MH  - Dendritic Cells
PMC - PMC10092987
OTO - NOTNLM
OT  - Phoneutria nigriventer
OT  - PnV
OT  - cell therapy
OT  - dendritic cells vaccine
OT  - glioblastoma
OT  - immunoadjuvant
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationship that could be construed as potential 
      conflict of interest.
EDAT- 2023/04/14 06:00
MHDA- 2023/04/14 06:41
PMCR- 2023/03/27
CRDT- 2023/04/13 01:16
PHST- 2022/08/18 00:00 [received]
PHST- 2023/01/04 00:00 [revised]
PHST- 2023/01/05 00:00 [accepted]
PHST- 2023/04/14 06:41 [medline]
PHST- 2023/04/13 01:16 [entrez]
PHST- 2023/04/14 06:00 [pubmed]
PHST- 2023/03/27 00:00 [pmc-release]
AID - cells12071023 [pii]
AID - cells-12-01023 [pii]
AID - 10.3390/cells12071023 [doi]
PST - epublish
SO  - Cells. 2023 Mar 27;12(7):1023. doi: 10.3390/cells12071023.