PMID- 37055645 OWN - NLM STAT- MEDLINE DCOM- 20231207 LR - 20231217 IS - 1573-7446 (Electronic) IS - 0165-7380 (Linking) VI - 47 IP - 4 DP - 2023 Dec TI - Lipid metabolites, interleukin-6 and oxidative stress markers in follicular fluid and their association with serum concentrations in mares. PG - 2221-2228 LID - 10.1007/s11259-023-10122-0 [doi] AB - The application of trans-vaginal ovum pick up (OPU) and intracytoplasmic sperm injection (ICSI) is well established for commercial in vitro embryo production in horses. These assisted reproductive techniques are especially applied during the non-breeding season of the mare. However, little is known about how the health of the oocyte donor may affect the biochemical composition of the follicular fluid (FF) in small and medium-sized follicles routinely aspirated during OPU. This study aimed to investigate associations between systemic and FF concentrations of interleukin-6 (IL-6), total cholesterol, triglycerides, non-esterified fatty acids (NEFA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSI) during the non-breeding season in mares. At the slaughterhouse, serum and FF of small (5-10 mm in diameter), medium (> 10-20 mm in diameter), and large (> 20-30 mm in diameter) follicles were sampled from 12 healthy mares. There was a strong positive association (P < 0.01) between the concentration of IL-6 in serum and those measured in small (r = 0.846), medium (r = 0.999), and large (r = 0.996) follicles. Serum concentrations of NEFA were positively correlated (P < 0.05) with those measured in small (r = 0.726), medium (r = 0.720), and large (r = 0.974) follicles. Values of total cholesterol and OSI in serum and medium follicles were significantly associated (r = 0.736 and r = 0.696, respectively). The serum concentrations of all lipid metabolites were markedly higher than those measured in FF of small- and medium-sized follicles. Values of IL-6 and OSI did not change significantly between serum and all follicle classes (P >/= 0.05). To conclude, changes in the blood composition associated with inflammation, oxidative stress, and disturbed lipid metabolism of mares may lead to an inadequate oocyte microenvironment, which could affect oocyte quality and the success rate of OPU/ICSI programs. Further research should indicate whether these changes may ultimately affect in vitro oocyte developmental capacity and subsequent embryo quality. CI - (c) 2023. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Hedia, Mohamed AU - Hedia M AUID- ORCID: 0000-0003-1806-2368 AD - Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium. mohammedhedia@cu.edu.eg. AD - Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. mohammedhedia@cu.edu.eg. AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. mohammedhedia@cu.edu.eg. FAU - Leroy, Jo L M R AU - Leroy JLMR AD - Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium. FAU - Govaere, Jan AU - Govaere J AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Van Soom, Ann AU - Van Soom A AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Smits, Katrien AU - Smits K AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. LA - eng PT - Journal Article DEP - 20230414 PL - Switzerland TA - Vet Res Commun JT - Veterinary research communications JID - 8100520 RN - 0 (Interleukin-6) RN - 0 (Fatty Acids, Nonesterified) RN - 97C5T2UQ7J (Cholesterol) SB - IM MH - Horses MH - Animals MH - Female MH - Male MH - *Follicular Fluid/chemistry/metabolism MH - *Interleukin-6/analysis/metabolism MH - Fatty Acids, Nonesterified/analysis/metabolism MH - Semen MH - Oxidative Stress MH - Cholesterol/analysis/metabolism MH - Oocytes/metabolism OTO - NOTNLM OT - Follicular fluid OT - IL-6 OT - Lipid metabolites OT - Mare OT - OPU/ICSI OT - Oxidative stress index EDAT- 2023/04/14 06:00 MHDA- 2023/12/07 12:42 CRDT- 2023/04/13 23:29 PHST- 2023/02/10 00:00 [received] PHST- 2023/04/10 00:00 [accepted] PHST- 2023/12/07 12:42 [medline] PHST- 2023/04/14 06:00 [pubmed] PHST- 2023/04/13 23:29 [entrez] AID - 10.1007/s11259-023-10122-0 [pii] AID - 10.1007/s11259-023-10122-0 [doi] PST - ppublish SO - Vet Res Commun. 2023 Dec;47(4):2221-2228. doi: 10.1007/s11259-023-10122-0. Epub 2023 Apr 14.