PMID- 37056772
OWN - NLM
STAT- MEDLINE
DCOM- 20230417
LR  - 20230417
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 14
DP  - 2023
TI  - Lipoteichoic acid of Streptococcus gordonii as a negative regulator of human 
      dendritic cell activation.
PG  - 1056949
LID - 10.3389/fimmu.2023.1056949 [doi]
LID - 1056949
AB  - Streptococcus gordonii, an opportunistic Gram-positive bacterium, causes an 
      infective endocarditis that could be fatal to human health. Dendritic cells (DCs) 
      are known to be involved in disease progression and immune responses in S. 
      gordonii infection. Since lipoteichoic acid (LTA) is a representative virulence 
      factor of S. gordonii, we here investigated its role in the activation of human 
      DCs stimulated with LTA-deficient (DeltaltaS) S. gordonii or S. gordonii LTA. DCs 
      were differentiated from human blood-derived monocytes in the presence of GM-CSF 
      and IL-4 for 6 days. DCs treated with heat-killed DeltaltaS S. gordonii (DeltaltaS HKSG) 
      showed relatively higher binding and phagocytic activities than those treated 
      with heat-killed wild-type S. gordonii (wild-type HKSG). Furthermore, DeltaltaS HKSG 
      was superior to wild-type HKSG in inducing phenotypic maturation markers 
      including CD80, CD83, CD86, PD-L1, and PD-L2, antigen-presenting molecule MHC 
      class II, and proinflammatory cytokines such as TNF-alpha and IL-6. Concomitantly, 
      DCs treated with the DeltaltaS HKSG induced better T cell activities, including 
      proliferation and activation marker (CD25) expression, than those treated with 
      the wild-type. LTA, but not lipoproteins, isolated from S. gordonii weakly 
      activated TLR2 and barely affected the expression of phenotypic maturation 
      markers or cytokines in DCs. Collectively, these results demonstrated that LTA is 
      not a major immuno-stimulating agent of S. gordonii but rather it interferes with 
      bacteria-induced DC maturation, suggesting its potential role in immune evasion.
CI  - Copyright (c) 2023 Kim, Im, Ko, Lee, Seo, Yun and Han.
FAU - Kim, Sun Kyung
AU  - Kim SK
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul, Republic of Korea.
FAU - Im, Jintaek
AU  - Im J
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul, Republic of Korea.
FAU - Ko, Eun Byeol
AU  - Ko EB
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul, Republic of Korea.
FAU - Lee, Dongwook
AU  - Lee D
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul, Republic of Korea.
FAU - Seo, Ho Seong
AU  - Seo HS
AD  - Research Division for Biotechnology, Korea Atomic Energy Research Institute, 
      Jeongeup, Republic of Korea.
FAU - Yun, Cheol-Heui
AU  - Yun CH
AD  - Department of Agricultural Biotechnology, and Research Institute of Agriculture 
      and Life Sciences, Seoul National University, Seoul, Republic of Korea.
AD  - Institutes of Green-bio Science and Technology, Seoul National University, 
      Pyeongchang, Gangwon-do, Republic of Korea.
AD  - Interdisciplinary Programs in Agricultural Genomics, Seoul National University, 
      Seoul, Republic of Korea.
FAU - Han, Seung Hyun
AU  - Han SH
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230328
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 56411-57-5 (lipoteichoic acid)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Humans
MH  - *Streptococcus gordonii/metabolism
MH  - *Cytokines/metabolism
MH  - Lipopolysaccharides/pharmacology/metabolism
MH  - Dendritic Cells
PMC - PMC10086370
OTO - NOTNLM
OT  - Streptococcus gordonii
OT  - T cell activation
OT  - dendritic cell
OT  - immune evasion
OT  - lipoprotein
OT  - lipoteichoic acid
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/04/15 06:00
MHDA- 2023/04/17 06:41
PMCR- 2023/01/01
CRDT- 2023/04/14 02:33
PHST- 2022/09/29 00:00 [received]
PHST- 2023/03/13 00:00 [accepted]
PHST- 2023/04/17 06:41 [medline]
PHST- 2023/04/14 02:33 [entrez]
PHST- 2023/04/15 06:00 [pubmed]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2023.1056949 [doi]
PST - epublish
SO  - Front Immunol. 2023 Mar 28;14:1056949. doi: 10.3389/fimmu.2023.1056949. 
      eCollection 2023.