PMID- 37057978
OWN - NLM
STAT- MEDLINE
DCOM- 20230615
LR  - 20230616
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 199
IP  - 6
DP  - 2023 Jun 1
TI  - RENEB Inter-Laboratory Comparison 2021: The FISH-Based Translocation Assay.
PG  - 583-590
LID - 10.1667/RADE-22-00203.1 [doi]
AB  - Translocation analysis using fluorescence in situ hybridization (FISH) is the 
      method of choice for dose assessment in case of chronic or past exposures to 
      ionizing radiation. Although it is a widespread technique, unlike dicentrics, the 
      number of FISH-based inter-laboratory comparisons is small. For this reason, 
      although the current Running the European Network of Biological and Physical 
      retrospective Dosimetry (RENEB) inter-laboratory comparison 2021 was designed as 
      a fast response to a real emergency scenario, it was considered a good 
      opportunity to perform an inter-laboratory comparison using the FISH technique to 
      gain further experience. The Bundeswehr Institute of Radiobiology provided 
      peripheral blood samples from one healthy human volunteer. Three test samples 
      were irradiated with blinded doses of 0, 1.2, and 3.5 Gy, respectively. Samples 
      were then sent to the seven participating laboratories. The FISH technique was 
      applied according to the standard procedure of each laboratory. Both, the 
      frequency of translocations and the estimated dose for each sample were sent to 
      the coordinator using a special scoring sheet for FISH. All participants sent 
      their results in due time. However, although it was initially requested to send 
      the results based on the full analysis, evaluating 500 equivalent cells, most 
      laboratories only sent the results based on triage, with a smaller number of 
      analyzed cells. In the triage analysis, there was great heterogeneity in the 
      number of equivalent cells scored. On the contrary, for the full analysis, this 
      number was more homogeneous. For all three samples, one laboratory showed outlier 
      yields compared to the other laboratories. Excluding these results, in the triage 
      analysis, the frequency of translocations in sample no. 1 ranged from 0 to 0.013 
      translocations per cell, and for samples no. 2 and no. 3 the genomic mean 
      frequency were 0.27 +/- 0.03 and 1.47 +/- 0.14, with a coefficient of variation of 
      0.29 and 0.23 respectively. Considering only results obtained in the triage 
      analysis for sample no. 1, all laboratories, except one, classified this sample 
      as the non-irradiated one. For sample no. 2, excluding the outlier value, the 
      mean reported dose was 1.74 +/- 0.16 Gy indicating a mean deviation of about 0.5 Gy 
      to the delivered dose of 1.2 Gy. For sample no. 3 the mean dose estimated was 
      4.21 +/- 0.21 Gy indicating a mean deviation of about 0.7 Gy to the delivered dose 
      of 3.5 Gy. In the frame of RENEB, this is the second FISH-based inter-laboratory 
      comparison. The whole exercise was planned as a response to an emergency, 
      therefore, a triage analysis was requested for all the biomarkers except for 
      FISH. Although a full analysis was initially requested for FISH, most of the 
      laboratories reported only a triage-based result. The main reason is that it was 
      not clearly stated what was required before starting the exercise. Results show 
      that most of the laboratories successfully discriminated unexposed and irradiated 
      samples from each other without any overlap. A good agreement in the observed 
      frequencies of translocations was observed but there was a tendency to 
      overestimate the delivered doses. Efforts to improve the harmonization of this 
      technique and subsequent exercises to elucidate the reason for this trend should 
      be promoted.
CI  - (c)2023 by Radiation Research Society. All rights of reproduction in any form 
      reserved.
FAU - Barquinero, J-F
AU  - Barquinero JF
AD  - Universitat Autonoma de Barcelona (UAB), Barcelona, Spain.
FAU - Abe, Y
AU  - Abe Y
AD  - Department of Radiation Biology and Protection, Atomic Bomb Disease Institute, 
      Nagasaki University (ABDI), Nagasaki, Japan.
FAU - Aneva, N
AU  - Aneva N
AD  - National Centre of Radiobiology and Radiation Protection (NCRRP), Sofia, 
      Bulgaria.
FAU - Endesfelder, D
AU  - Endesfelder D
AD  - Bundesamt fur Strahlenschutz (BfS), Oberschleissheim, Germany.
FAU - Georgieva, D
AU  - Georgieva D
AD  - National Centre of Radiobiology and Radiation Protection (NCRRP), Sofia, 
      Bulgaria.
FAU - Goh, Vst
AU  - Goh V
AD  - Singapore Nuclear Research and Safety Initiative (SNRSI), Singapore.
FAU - Gregoire, E
AU  - Gregoire E
AD  - Institut de Radioprotection et de Surete Nucleaire (IRSN), Fontenay aux Roses, 
      France.
FAU - Hristova, R
AU  - Hristova R
AD  - National Centre of Radiobiology and Radiation Protection (NCRRP), Sofia, 
      Bulgaria.
FAU - Lee, Y
AU  - Lee Y
AD  - Korea Institute of Radiological & Medical Sciences, Laboratory of Biological 
      Dosimetry (KIRAMS), Seoul, Korea.
FAU - Martinez, J-S
AU  - Martinez JS
AD  - Institut de Radioprotection et de Surete Nucleaire (IRSN), Fontenay aux Roses, 
      France.
FAU - Meher, P-K
AU  - Meher PK
AD  - Centre for Radiation Protection Research, Department of Molecular Biosciences, 
      The Wenner-Gren Institute, Stockholm University (SU), Stockholm, Sweden.
FAU - Miura, T
AU  - Miura T
AD  - Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency 
      Medicine, Hirosaki University (IREM), Aomori, Japan.
FAU - Port, M
AU  - Port M
AD  - Bundeswehr Institute of Radiobiology affiliated to the University of Ulm (BIR), 
      Munich, Germany.
FAU - Pujol-Canadell, M
AU  - Pujol-Canadell M
AD  - Universitat Autonoma de Barcelona (UAB), Barcelona, Spain.
FAU - Prieto-Rodriguez, M J
AU  - Prieto-Rodriguez MJ
AD  - Hospital General Universitario Gregorio Maranon, Laboratorio de dosimetria 
      biologica (SERMAS), Madrid, Spain.
FAU - Seong, K-M
AU  - Seong KM
AD  - Institut de Radioprotection et de Surete Nucleaire (IRSN), Fontenay aux Roses, 
      France.
FAU - Suto, Y
AU  - Suto Y
AD  - Biodosimetry Group, National Institutes for Quantum Science and Technology (QST), 
      Chiba, Japan.
FAU - Takebayashi, K
AU  - Takebayashi K
AD  - Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency 
      Medicine, Hirosaki University (IREM), Aomori, Japan.
FAU - Tsuyama, N
AU  - Tsuyama N
AD  - Department of Radiation Life Sciences, Fukushima Medical University (FMU), 
      Fukushima, Japan.
FAU - Wojcik, A
AU  - Wojcik A
AD  - Centre for Radiation Protection Research, Department of Molecular Biosciences, 
      The Wenner-Gren Institute, Stockholm University (SU), Stockholm, Sweden.
FAU - Yoon, H-J
AU  - Yoon HJ
AD  - Korea Institute of Radiological & Medical Sciences, Laboratory of Biological 
      Dosimetry (KIRAMS), Seoul, Korea.
FAU - Abend, M
AU  - Abend M
AD  - Bundeswehr Institute of Radiobiology affiliated to the University of Ulm (BIR), 
      Munich, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Retrospective Studies
MH  - *Translocation, Genetic
MH  - *Radiometry/methods
MH  - Biological Assay/methods
MH  - Chromosome Aberrations
EDAT- 2023/04/15 06:00
MHDA- 2023/06/15 06:41
CRDT- 2023/04/14 09:42
PHST- 2022/11/28 00:00 [received]
PHST- 2023/03/07 00:00 [accepted]
PHST- 2023/06/15 06:41 [medline]
PHST- 2023/04/15 06:00 [pubmed]
PHST- 2023/04/14 09:42 [entrez]
AID - 492245 [pii]
AID - 10.1667/RADE-22-00203.1 [doi]
PST - ppublish
SO  - Radiat Res. 2023 Jun 1;199(6):583-590. doi: 10.1667/RADE-22-00203.1.