PMID- 37058773
OWN - NLM
STAT- MEDLINE
DCOM- 20230526
LR  - 20230526
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 23
IP  - 2
DP  - 2023 Jun
TI  - Role of kisspeptin-10 and betacellulin in control of feline ovarian cell 
      functions.
PG  - 100762
LID - S1642-431X(23)00034-7 [pii]
LID - 10.1016/j.repbio.2023.100762 [doi]
AB  - The action of betacellulin (BTC) on basic ovarian cell functions and 
      interrelationships with kisspeptin (KISS) was investigated. For this purpose, we 
      examined (1) the effect of the addition of BTC (0, 1, 10, and 100 ng/ml) given 
      alone or in combination with KISS (10 ng/ml) on cultured feline ovarian fragments 
      or granulosa cells. Viability, proliferation (accumulation of cyclin B1) and 
      apoptosis (accumulation of bax), and the release of steroid hormones 
      (progesterone, testosterone, and estradiol) were analyzed by using the Trypan 
      blue exclusion test, quantitative immunocytochemistry, and ELISA. The addition of 
      KISS alone increased proliferation, apoptosis, progesterone, estradiol release, 
      and decreased testosterone but did not affect viability. The addition of BTC 
      alone decreased cell proliferation, apoptosis, progesterone, testosterone, and 
      estradiol release but did not influence viability. Furthermore, BTC mainly 
      inhibited the stimulatory action of KISS on feline ovarian functions. The 
      findings of our study suggest the effects of KISS on basic ovarian functions. We 
      also observed the influence of BTC on these functions and its ability to modify 
      the effects of KISS on these processes.
CI  - Copyright (c) 2023 Society for Biology of Reproduction & the Institute of Animal 
      Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. 
      Published by Elsevier B.V. All rights reserved.
FAU - Loncova, Barbora
AU  - Loncova B
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Nitra, Slovakia. Electronic address: barbora.loncova@ukf.sk.
FAU - Fabova, Zuzana
AU  - Fabova Z
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Nitra, Slovakia.
FAU - Sirotkin, Alexander V
AU  - Sirotkin AV
AD  - Department of Zoology and Anthropology, Constantine the Philosopher University, 
      Nitra, Slovakia.
LA  - eng
PT  - Journal Article
DEP - 20230412
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 0 (KISS1 protein, human)
RN  - 0 (Kisspeptins)
RN  - 0 (Betacellulin)
RN  - 4TI98Z838E (Estradiol)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Female
MH  - Cats
MH  - Animals
MH  - *Progesterone
MH  - *Kisspeptins/pharmacology
MH  - Betacellulin
MH  - Estradiol
MH  - Testosterone
OTO - NOTNLM
OT  - Betacellulin
OT  - Feline
OT  - Kisspeptin
OT  - Ovary
COIS- Conflicts of interest The authors confirm the lack of potential conflicts of 
      interest.
EDAT- 2023/04/15 06:00
MHDA- 2023/05/26 06:42
CRDT- 2023/04/14 18:00
PHST- 2022/10/06 00:00 [received]
PHST- 2023/03/28 00:00 [revised]
PHST- 2023/04/01 00:00 [accepted]
PHST- 2023/05/26 06:42 [medline]
PHST- 2023/04/15 06:00 [pubmed]
PHST- 2023/04/14 18:00 [entrez]
AID - S1642-431X(23)00034-7 [pii]
AID - 10.1016/j.repbio.2023.100762 [doi]
PST - ppublish
SO  - Reprod Biol. 2023 Jun;23(2):100762. doi: 10.1016/j.repbio.2023.100762. Epub 2023 
      Apr 12.