PMID- 37062343
OWN - NLM
STAT- MEDLINE
DCOM- 20230508
LR  - 20230508
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 228
DP  - 2023 Jun 1
TI  - Aflatoxin B(1) can aggravate BALB/c mice allergy to ovalbumin through changing 
      their Th2 cells immune responses.
PG  - 107121
LID - S0041-0101(23)00107-1 [pii]
LID - 10.1016/j.toxicon.2023.107121 [doi]
AB  - Foods contaminated by Aflatoxin B(1) (AFB(1)) frequently happen in the world and 
      can cause a lot healthy damages to human beings, meanwhile, some of these foods 
      are easily irritate food allergy. To investigate the effect of AFB(1) exposure on 
      food allergy, three doses of AFB(1) were set, including 0.3 mug/kg . bw (LDAF), 
      7.5 mug/kg . bw (MDAF), and 100.0 mug/kg . bw (HDAF), respectively; food allergy 
      model was constructed by the BALB/c mice allergy to ovalbumin (OVA). The changes 
      of titer in OVA-specific immunoglobulin E (IgE), IgG, IgG1, IgG2a, as well as 
      level of the mMCP-1 in sera were determined by enzyme linked immunosorbent assay 
      (ELISA), respectively; the levels of interleukin (IL-4, IL-5, IL-13) and 
      interferon (IFN)-gamma in spleen were separately assessed using ELISA kits, and their 
      relative genes expression were verified by Real-time fluorescence quantitative 
      PCR (Q-PCR); the population of Th1/Th2/Treg cells were analyzed by flow 
      cytometry. Results showed that when OVA-allergic mice were exposed to AFB(1), the 
      production of OVA-specific IgE, IL-4, IL-5 and IL-13, and mMCP-1 were all 
      increased, whereas the level of IFN-gamma was decreased; the Th1/Th2 balance was 
      disrupted and the development of Th cells tilted to the Th2 phenotype. The study 
      would contribute to further understand the risk of fungal toxins in food allergy.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Deng, Yujue
AU  - Deng Y
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, PR China; College of Food Science & Technology, Nanchang 
      University, Nanchang, 330031, PR China.
FAU - Chen, Hongbing
AU  - Chen H
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang 
      University, Nanchang, 330047, PR China.
FAU - Wu, Yong
AU  - Wu Y
AD  - Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, 330047, PR 
      China.
FAU - Yuan, Jin
AU  - Yuan J
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, PR China; College of Food Science & Technology, Nanchang 
      University, Nanchang, 330031, PR China.
FAU - Shi, Qiang
AU  - Shi Q
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, PR China; College of Food Science & Technology, Nanchang 
      University, Nanchang, 330031, PR China.
FAU - Tong, Ping
AU  - Tong P
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, PR China. Electronic address: tongping@ncu.edu.cn.
FAU - Gao, Jinyan
AU  - Gao J
AD  - College of Food Science & Technology, Nanchang University, Nanchang, 330031, PR 
      China. Electronic address: gaoyj2013@ncu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230414
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 9006-59-1 (Ovalbumin)
RN  - 9N2N2Y55MH (Aflatoxin B1)
RN  - 0 (Interleukin-13)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Interleukin-5)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Cytokines)
SB  - IM
MH  - Mice
MH  - Humans
MH  - Animals
MH  - Ovalbumin/pharmacology
MH  - *Th2 Cells
MH  - Aflatoxin B1/toxicity
MH  - Mice, Inbred BALB C
MH  - Interleukin-13/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Interleukin-5/pharmacology
MH  - *Food Hypersensitivity
MH  - Immunoglobulin E
MH  - Immunoglobulin G
MH  - Immunity
MH  - Cytokines
OTO - NOTNLM
OT  - Aflatoxin B(1)
OT  - Cytokine
OT  - Food allergy
OT  - IgE
OT  - Oral ministration
OT  - Ovalbumin
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/04/17 06:00
MHDA- 2023/05/08 06:42
CRDT- 2023/04/16 19:21
PHST- 2023/01/31 00:00 [received]
PHST- 2023/04/07 00:00 [revised]
PHST- 2023/04/08 00:00 [accepted]
PHST- 2023/05/08 06:42 [medline]
PHST- 2023/04/17 06:00 [pubmed]
PHST- 2023/04/16 19:21 [entrez]
AID - S0041-0101(23)00107-1 [pii]
AID - 10.1016/j.toxicon.2023.107121 [doi]
PST - ppublish
SO  - Toxicon. 2023 Jun 1;228:107121. doi: 10.1016/j.toxicon.2023.107121. Epub 2023 Apr 
      14.