PMID- 37062850
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231106
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Apr 16
TI  - CAFs-derived rho-associated kinase1 mediated EMT to promote laryngeal squamous 
      cell carcinoma metastasis.
PG  - 70
LID - 10.1186/s12935-023-02911-z [doi]
LID - 70
AB  - BACKGROUND: Cancer-associated fibroblasts (CAFs) play an essential role in 
      tumorigenesis and development of cancers. Nevertheless, the specific molecular 
      mechanism of tumorigenesis and development in Laryngeal squamous cell carcinoma 
      (LSCC) still unknown. METHODS: CAFs, CPFs and NFs were isolated and identified 
      from laryngeal cancer, para-laryngeal cancer and normal tissues. 
      Immunofluorescent staining, Rt-PCR and Western Blot were used to detect the 
      expression of related proteins. Wound healing, migration, invasion and animal 
      experiments were used to examine the ability of movement, migration, invasion and 
      metastasis of LSCC. RESULTS: ROCK1, was highly expressed in CAFs and CAFs 
      enhanced LSCC metastasis in vivo and vitro, and downregulation of ROCK1 in CAFs 
      inhibited the migration and invasion of LSCC cells. While increasing ROCK1 
      expression in NFs promoted the migration and invasion of LSCC cells. Further 
      studies revealed that epithelial-mesenchymal transition (EMT) and 
      JAK2/STAT3/ERK1/2 pathway might play an essential role in promoting metastasis of 
      LSCC. In addition, inhibition activity of ROCK1 or JAK2/STAT3/ERK1/2 signal 
      molecules significantly reduced EMT and metastasis. CONCLUSIONS: CAFs-derived 
      ROCK1 via JAK2/STAT3/ERK1/2 axis mediated EMT to promote LSCC metastasis and 
      targeting ROCK1 might provide a potential treatment strategy for LSCC.
CI  - (c) 2023. The Author(s).
FAU - Yang, Liyun
AU  - Yang L
AD  - Department of Otolaryngology, Gongli Hospital, the Second Military Medical 
      University, Shanghai, 200135, China.
FAU - Gong, Shiqi
AU  - Gong S
AD  - Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, Shanghai, 200025, China.
FAU - Qiao, Peipei
AU  - Qiao P
AD  - Department of Otolaryngology, Gongli Hospital, the Second Military Medical 
      University, Shanghai, 200135, China.
FAU - Zhao, Runyu
AU  - Zhao R
AD  - Postgraduate Training Base at Shanghai Gongli Hospital, Ningxia Medical 
      University, Shanghai, 200135, China.
FAU - Huang, Shuixian
AU  - Huang S
AD  - Department of Otolaryngology, Gongli Hospital, the Second Military Medical 
      University, Shanghai, 200135, China. hsxent@163.com.
FAU - Zhou, Jieyu
AU  - Zhou J
AD  - Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, Ear 
      Institute, Shanghai Jiaotong University School of Medicine, Shanghai, China, 
      Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, 
      Shanghai, 200011, China. entzhoujieyu@126.com.
FAU - Hu, An
AU  - Hu A
AD  - Department of Otolaryngology, Gongli Hospital, the Second Military Medical 
      University, Shanghai, 200135, China. juanitohuan@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230416
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC10105957
OTO - NOTNLM
OT  - CAFs
OT  - EMT
OT  - JAK2/STAT3/ERK1/2 signal pathway
OT  - Metastasis
OT  - ROCK1
COIS- There is on competing interests.
EDAT- 2023/04/17 06:00
MHDA- 2023/04/17 06:01
PMCR- 2023/04/16
CRDT- 2023/04/16 23:44
PHST- 2023/02/01 00:00 [received]
PHST- 2023/03/28 00:00 [accepted]
PHST- 2023/04/17 06:01 [medline]
PHST- 2023/04/16 23:44 [entrez]
PHST- 2023/04/17 06:00 [pubmed]
PHST- 2023/04/16 00:00 [pmc-release]
AID - 10.1186/s12935-023-02911-z [pii]
AID - 2911 [pii]
AID - 10.1186/s12935-023-02911-z [doi]
PST - epublish
SO  - Cancer Cell Int. 2023 Apr 16;23(1):70. doi: 10.1186/s12935-023-02911-z.