PMID- 37065194
OWN - NLM
STAT- MEDLINE
DCOM- 20230418
LR  - 20230421
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 13
DP  - 2023
TI  - A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck 
      adenovirus 3.
PG  - 1177866
LID - 10.3389/fcimb.2023.1177866 [doi]
LID - 1177866
AB  - Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck 
      adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic 
      losses to the duck industry. Therefore, there is an urgent need to generate a 
      recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. 
      In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of 
      DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 
      and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot 
      (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was 
      expressed successfully. Moreover, the growth curve revealed that 
      rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a 
      stronger replication ability compared to the wild type FAdV-4. The generation of 
      the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate 
      against both FAdV-4 and DAdV-3.
CI  - Copyright (c) 2023 Guo, Lin, Xie, Zhang, Xu, Chao, Cao, Jiang, Li, Li, Wan, Shao, 
      Qin and Ye.
FAU - Guo, Yiwen
AU  - Guo Y
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Lin, Yun
AU  - Lin Y
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
FAU - Xie, Quan
AU  - Xie Q
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Zhang, Wenyuan
AU  - Zhang W
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Xu, Zhenqi
AU  - Xu Z
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Chao, Yifei
AU  - Chao Y
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Cao, Xudong
AU  - Cao X
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Jiang, Huiru
AU  - Jiang H
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Li, Han
AU  - Li H
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Li, Tuofan
AU  - Li T
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Wan, Zhimin
AU  - Wan Z
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Shao, Hongxia
AU  - Shao H
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Qin, Aijian
AU  - Qin A
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Ye, Jianqiang
AU  - Ye J
AD  - Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for 
      Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 
      China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230328
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Ducks
MH  - *Adenoviridae Infections/pathology
MH  - Serogroup
MH  - Antibodies, Viral
MH  - Chickens
MH  - *Aviadenovirus/genetics
MH  - *Vaccines
MH  - *Poultry Diseases
PMC - PMC10090666
OTO - NOTNLM
OT  - CRISPR/Cas9
OT  - DAdV-3
OT  - expression
OT  - fiber-2
OT  - recombinant FAdV-4
OT  - replication
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/04/18 06:00
MHDA- 2023/04/18 10:16
PMCR- 2023/01/01
CRDT- 2023/04/17 03:53
PHST- 2023/03/02 00:00 [received]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/04/18 10:16 [medline]
PHST- 2023/04/17 03:53 [entrez]
PHST- 2023/04/18 06:00 [pubmed]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2023.1177866 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2023 Mar 28;13:1177866. doi: 
      10.3389/fcimb.2023.1177866. eCollection 2023.