PMID- 37068390
OWN - NLM
STAT- MEDLINE
DCOM- 20230501
LR  - 20230502
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 660
DP  - 2023 Jun 11
TI  - Role of the blood-spinal cord barrier: An adheren junction regulation mechanism 
      that promotes chronic postsurgical pain.
PG  - 65-72
LID - S0006-291X(23)00422-9 [pii]
LID - 10.1016/j.bbrc.2023.04.021 [doi]
AB  - Chronic postsurgical pain (CPSP) is a serious postoperative complication with 
      high incidence, and its pathogenesis involves neuroimmune interactions and the 
      breakdown of the blood-spinal cord barrier (BSCB), the decreased level of adheren 
      junction (AJ)-related proteins is an important cause of BSCB injury. Vascular 
      endothelial-cadherin (VE-cadherin) and p120 catenin (p120) constitute the 
      endothelial barrier adheren junction. The Src/p120/VE-cadherin pathway is 
      involved in the regulation of the endothelial barrier function. However, the role 
      of the BSCB-AJ regulatory mechanism in CPSP has not been reported. In this study, 
      we established a skin/muscle incision and retraction (SMIR) model and evaluated 
      the paw withdrawal threshold (PWT), the effects of an Src inhibitor and p120 
      knockdown on p-Src, p120 and VE-cadherin expression, as well as BSCB-AJ function 
      in rat spinal cord were observed to explore the regulation of BSCB-AJ function by 
      the p-Src/p120/VE-cadherin pathway in promoting SMIR-induced CPSP. The levels of 
      p-Src, p120 and VE-cadherin in the spinal cord were detected by Western blot. 
      Meanwhile, BSCB permeability test was used to detect the changes in BCSB 
      function. Finally, the spatial and temporal localization of p120 in spinal cord 
      was detected by immunofluorescence. Our findings indicated that 
      p-Src/p120/VE-cadherin could induce BSCB-AJ dysfunction and promote the 
      development of CPSP.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Zhao, Qihong
AU  - Zhao Q
AD  - Medical College, Nantong University, Nantong, Jiangsu, 226001, China.
FAU - Yao, Ju
AU  - Yao J
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu, 226001, China.
FAU - Meng, Bei
AU  - Meng B
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu, 226001, China.
FAU - Shen, Shiren
AU  - Shen S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu, 226001, China.
FAU - Cao, Su
AU  - Cao S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu, 226001, China. Electronic address: 
      5200439@ntu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230411
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cadherins)
RN  - 0 (Catenins)
RN  - 0 (Delta Catenin)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Cadherins/metabolism
MH  - *Catenins/metabolism
MH  - Delta Catenin
MH  - Spinal Cord/metabolism
MH  - Fetal Blood/metabolism
MH  - Pain, Postoperative
OTO - NOTNLM
OT  - Adheren junction
OT  - Blood-spinal cord barrier
OT  - Chronic postsurgical pain
OT  - Neurovascular unit
OT  - VE-cadherin
OT  - p120
COIS- Declaration of competing interest The authors have no conflicts of interest to 
      declare.
EDAT- 2023/04/18 06:00
MHDA- 2023/05/01 06:42
CRDT- 2023/04/17 18:04
PHST- 2023/03/02 00:00 [received]
PHST- 2023/03/16 00:00 [revised]
PHST- 2023/04/10 00:00 [accepted]
PHST- 2023/05/01 06:42 [medline]
PHST- 2023/04/18 06:00 [pubmed]
PHST- 2023/04/17 18:04 [entrez]
AID - S0006-291X(23)00422-9 [pii]
AID - 10.1016/j.bbrc.2023.04.021 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2023 Jun 11;660:65-72. doi: 
      10.1016/j.bbrc.2023.04.021. Epub 2023 Apr 11.