PMID- 37069579
OWN - NLM
STAT- MEDLINE
DCOM- 20230419
LR  - 20230421
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 54
IP  - 1
DP  - 2023 Apr 17
TI  - Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral 
      haemorrhagic septicaemia virus facilitated by genome sequencing of carpione 
      novirhabdovirus.
PG  - 35
LID - 10.1186/s13567-023-01166-w [doi]
LID - 35
AB  - The monoclonal antibody (mAb) IP5B11, which is used worldwide for the diagnosis 
      of viral haemorrhagic septicaemia (VHS) in fish, reacts with all genotypes of VHS 
      virus (VHSV). The mAb exceptionally also reacts with the carpione rhabdovirus 
      (CarRV). Following next generation genome sequencing of CarRV and N protein 
      sequence alignment including five kinds of fish novirhabdoviruses, the epitope 
      recognized by mAb IP5B11 was identified. Dot blot analysis confirmed the epitope 
      of mAb IP5B11 to be associated with the region N219 to N233 of the N protein of 
      VHSV. Phylogenetic analysis identified CarRV as a new member of the fish 
      novirhabdoviruses.
CI  - (c) 2023. The Author(s).
FAU - Ito, Takafumi
AU  - Ito T
AUID- ORCID: 0000-0001-6275-1131
AD  - Pathology Division, Fisheries Research Agency, Fisheries Technology Institute, 
      422-1 Nakatsuhamaura, Minami-Ise, Mie, 516-0193, Japan. ito_takafumi99@fra.go.jp.
FAU - Mekata, Tohru
AU  - Mekata T
AD  - Pathology Division, Fisheries Research Agency, Fisheries Technology Institute, 
      422-1 Nakatsuhamaura, Minami-Ise, Mie, 516-0193, Japan.
AD  - Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, 
      Imabari, Ehime, Japan.
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
AD  - National Institute for Aquatic Resources, Technical University of Denmark, 
      Kemitorvet 202, 2800, Kgs Lyngby, Denmark.
FAU - Lorenzen, Niels
AU  - Lorenzen N
AD  - National Institute for Aquatic Resources, Technical University of Denmark, 
      Kemitorvet 202, 2800, Kgs Lyngby, Denmark.
LA  - eng
GR  - 20K06218/JSPS KAKENHI/
GR  - 9041-00227B/The Independent Research Fund Denmark/
PT  - Journal Article
DEP - 20230417
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - *Novirhabdovirus/genetics
MH  - Antibodies, Monoclonal
MH  - Epitope Mapping/veterinary
MH  - Phylogeny
MH  - Fishes
MH  - Epitopes
MH  - *Fish Diseases/diagnosis
PMC - PMC10111850
OTO - NOTNLM
OT  - Carpione rhabdovirus
OT  - Epitope mapping
OT  - VHSV
OT  - mAb IP5B11
COIS- The authors declare that they have no competing interests.
EDAT- 2023/04/19 06:00
MHDA- 2023/04/19 06:42
PMCR- 2023/04/17
CRDT- 2023/04/18 00:07
PHST- 2022/12/15 00:00 [received]
PHST- 2023/03/29 00:00 [accepted]
PHST- 2023/04/19 06:42 [medline]
PHST- 2023/04/18 00:07 [entrez]
PHST- 2023/04/19 06:00 [pubmed]
PHST- 2023/04/17 00:00 [pmc-release]
AID - 10.1186/s13567-023-01166-w [pii]
AID - 1166 [pii]
AID - 10.1186/s13567-023-01166-w [doi]
PST - epublish
SO  - Vet Res. 2023 Apr 17;54(1):35. doi: 10.1186/s13567-023-01166-w.