PMID- 37069673
OWN - NLM
STAT- MEDLINE
DCOM- 20230419
LR  - 20230421
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 14
IP  - 1
DP  - 2023 Apr 17
TI  - Low-intensity pulsed ultrasound promotes mesenchymal stem cell 
      transplantation-based articular cartilage regeneration via inhibiting the TNF 
      signaling pathway.
PG  - 93
LID - 10.1186/s13287-023-03296-6 [doi]
LID - 93
AB  - BACKGROUND: Mesenchymal stem cell (MSC) transplantation therapy is highly 
      investigated for the regenerative repair of cartilage defects. Low-intensity 
      pulsed ultrasound (LIPUS) has the potential to promote chondrogenic 
      differentiation of MSCs. However, its underlying mechanism remains unclear. Here, 
      we investigated the promoting effects and mechanisms underlying LIPUS stimulation 
      on the chondrogenic differentiation of human umbilical cord mesenchymal stem 
      cells (hUC-MSCs) and further evaluated its regenerative application value in 
      articular cartilage defects in rats. METHODS: LIPUS was applied to stimulate 
      cultured hUC-MSCs and C28/I2 cells in vitro. Immunofluorescence staining, qPCR 
      analysis, and transcriptome sequencing were used to detect mature 
      cartilage-related markers of gene and protein expression for a comprehensive 
      evaluation of differentiation. Injured articular cartilage rat models were 
      established for further hUC-MSC transplantation and LIPUS stimulation in vivo. 
      Histopathology and H&E staining were used to evaluate the repair effects of the 
      injured articular cartilage with LIPUS stimulation. RESULTS: The results showed 
      that LIPUS stimulation with specific parameters effectively promoted the 
      expression of mature cartilage-related genes and proteins, inhibited TNF-alpha gene 
      expression in hUC-MSCs, and exhibited anti-inflammation in C28/I2 cells. In 
      addition, the articular cartilage defects of rats were significantly repaired 
      after hUC-MSC transplantation and LIPUS stimulation. CONCLUSIONS: Taken together, 
      LIPUS stimulation could realize articular cartilage regeneration based on hUC-MSC 
      transplantation due to the inhibition of the TNF signaling pathway, which is of 
      clinical value for the relief of osteoarthritis.
CI  - (c) 2023. The Author(s).
FAU - Chen, Yiming
AU  - Chen Y
AD  - Institute of Acoustics, School of Physics Science and Engineering, Tongji 
      University, Shanghai, 200092, China.
FAU - Yang, Huiyi
AU  - Yang H
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to 
      Tongji University School of Medicine, Tongji University, Shanghai, 200065, China.
FAU - Wang, Zhaojie
AU  - Wang Z
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to 
      Tongji University School of Medicine, Tongji University, Shanghai, 200065, China.
AD  - School of Life Science and Technology, Tongji University, Shanghai, 200065, 
      China.
FAU - Zhu, Rongrong
AU  - Zhu R
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to 
      Tongji University School of Medicine, Tongji University, Shanghai, 200065, China.
AD  - School of Life Science and Technology, Tongji University, Shanghai, 200065, 
      China.
FAU - Cheng, Liming
AU  - Cheng L
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to 
      Tongji University School of Medicine, Tongji University, Shanghai, 200065, China. 
      limingcheng@tongji.edu.cn.
FAU - Cheng, Qian
AU  - Cheng Q
AUID- ORCID: 0000-0003-3621-5743
AD  - Institute of Acoustics, School of Physics Science and Engineering, Tongji 
      University, Shanghai, 200092, China. q.cheng@tongji.edu.cn.
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to 
      Tongji University School of Medicine, Tongji University, Shanghai, 200065, China. 
      q.cheng@tongji.edu.cn.
AD  - Frontiers Science Center for Intelligent Autonomous Systems, Shanghai, 201210, 
      China. q.cheng@tongji.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230417
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
SB  - IM
MH  - Rats
MH  - Humans
MH  - Animals
MH  - *Cartilage, Articular/pathology
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - *Mesenchymal Stem Cells
MH  - Signal Transduction
MH  - Ultrasonic Waves
PMC - PMC10111837
OTO - NOTNLM
OT  - Articular cartilage regeneration
OT  - Chondrogenic differentiation
OT  - Low-intensity pulsed ultrasound (LIPUS)
OT  - Mesenchymal stem cells (MSCs)
OT  - TNF signaling pathway
COIS- The authors declare no competing interests.
EDAT- 2023/04/19 06:00
MHDA- 2023/04/19 06:42
PMCR- 2023/04/17
CRDT- 2023/04/18 00:13
PHST- 2022/02/26 00:00 [received]
PHST- 2023/03/22 00:00 [accepted]
PHST- 2023/04/19 06:42 [medline]
PHST- 2023/04/18 00:13 [entrez]
PHST- 2023/04/19 06:00 [pubmed]
PHST- 2023/04/17 00:00 [pmc-release]
AID - 10.1186/s13287-023-03296-6 [pii]
AID - 3296 [pii]
AID - 10.1186/s13287-023-03296-6 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2023 Apr 17;14(1):93. doi: 10.1186/s13287-023-03296-6.