PMID- 37069968
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230419
IS  - 2468-0249 (Electronic)
IS  - 2468-0249 (Linking)
VI  - 8
IP  - 4
DP  - 2023 Apr
TI  - Urinary T Cells Identify Renal Antineutrophil Cytoplasmic Antibody-Associated 
      Vasculitis and Predict Prognosis: A Proof of Concept Study.
PG  - 871-883
LID - 10.1016/j.ekir.2023.01.013 [doi]
AB  - INTRODUCTION: Necrotizing crescentic glomerulonephritis is a major contributor to 
      morbidity and mortality in Antineutrophil cytoplasmic antibodies 
      (ANCA)-associated vasculitis (AAV). Because therapy relies on immunosuppressive 
      agents with potentially severe adverse effects, a reliable noninvasive biomarker 
      of disease activity is needed to guide treatment. METHODS: We used flow cytometry 
      to quantify T cell subsets in blood and urine samples from 95 patients with AAV 
      and 8 controls to evaluate their biomarker characteristics. These were compared 
      to soluble markers, monocyte chemoattractant protein-1 (MCP-1), soluble CD163 
      (sCD163), soluble CD25 (sCD25), and complement C5a (C5a), measured using 
      multiplex analysis. Available kidney biopsies (n = 21) were classified according 
      to Berden. RESULTS: Patients with active renal AAV (rAAV) showed significantly 
      higher urinary cell counts than those in remission, or those with extrarenal 
      manifestation, or healthy controls. Urinary T cells showed robust discrimination 
      of disease activity with superior performance compared to MCP-1 and sCD163. 
      Patients whose kidney biopsies had been classified as "crescentic" according to 
      Berden classification showed higher urinary T cell counts. Discordant regulatory 
      T cells (T(reg)) proportions and CD4(+)/CD8(+) ratio in blood and urine suggested 
      that urinary cells reflect tissue migration rather than mere micro-bleeding. 
      Furthermore, urinary T(reg) and T helper cells (T(H)17) patterns were associated 
      with clinical response and risk of renal relapse. CONCLUSION: Urinary T cells 
      reflect the renal inflammatory milieu in AAV and provide further insights into 
      the pathogenesis of this chronic condition. Their promising potential as 
      noninvasive diagnostic and prognostic biomarkers deserves further exploitation.
CI  - (c) 2023 International Society of Nephrology. Published by Elsevier Inc.
FAU - Sonnemann, Janis
AU  - Sonnemann J
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
AD  - Experimental and Clinical Research Center, Charite-Universitatsmedizin Berlin and 
      Max Delbruck Center for Molecular Medicine in the Helmholtz Association, Berlin, 
      Germany.
AD  - Berlin Institute of Health, Berlin, Germany.
FAU - Klocke, Jan
AU  - Klocke J
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Bieringer, Markus
AU  - Bieringer M
AD  - Department of Nephrology, Helios Klinikum Berlin-Buch, Berlin, Germany.
FAU - Rousselle, Anthony
AU  - Rousselle A
AD  - Experimental and Clinical Research Center, Charite-Universitatsmedizin Berlin and 
      Max Delbruck Center for Molecular Medicine in the Helmholtz Association, Berlin, 
      Germany.
FAU - Eckardt, Kai-Uwe
AU  - Eckardt KU
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Elitok, Saban
AU  - Elitok S
AD  - Department of Nephrology and Endocrinology, Ernst von Bergmann Klinikum, Potsdam, 
      Germany.
FAU - Popovic, Suncica
AU  - Popovic S
AD  - Department of Anatomy, Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Bachmann, Sebastian
AU  - Bachmann S
AD  - Department of Anatomy, Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Kettritz, Ralph
AU  - Kettritz R
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
AD  - Experimental and Clinical Research Center, Charite-Universitatsmedizin Berlin and 
      Max Delbruck Center for Molecular Medicine in the Helmholtz Association, Berlin, 
      Germany.
FAU - Salama, Alan D
AU  - Salama AD
AD  - Center for Nephrology, University College London, Royal Free Hospital, London, 
      UK.
FAU - Enghard, Philipp
AU  - Enghard P
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
AD  - Deutsches Rheumaforschungszentrum Berlin (DRFZ).
FAU - Schreiber, Adrian
AU  - Schreiber A
AD  - Department of Nephrology and Medical Intensive Care, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
AD  - Experimental and Clinical Research Center, Charite-Universitatsmedizin Berlin and 
      Max Delbruck Center for Molecular Medicine in the Helmholtz Association, Berlin, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20230118
PL  - United States
TA  - Kidney Int Rep
JT  - Kidney international reports
JID - 101684752
PMC - PMC10105048
OTO - NOTNLM
OT  - ANCA
OT  - TH
OT  - Treg
OT  - vasculitis
EDAT- 2023/04/19 06:00
MHDA- 2023/04/19 06:01
PMCR- 2023/01/18
CRDT- 2023/04/18 01:45
PHST- 2022/08/15 00:00 [received]
PHST- 2022/12/19 00:00 [revised]
PHST- 2023/01/05 00:00 [accepted]
PHST- 2023/04/19 06:01 [medline]
PHST- 2023/04/18 01:45 [entrez]
PHST- 2023/04/19 06:00 [pubmed]
PHST- 2023/01/18 00:00 [pmc-release]
AID - S2468-0249(23)00014-1 [pii]
AID - 10.1016/j.ekir.2023.01.013 [doi]
PST - epublish
SO  - Kidney Int Rep. 2023 Jan 18;8(4):871-883. doi: 10.1016/j.ekir.2023.01.013. 
      eCollection 2023 Apr.