PMID- 37074662
OWN - NLM
STAT- MEDLINE
DCOM- 20230421
LR  - 20230426
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2635
DP  - 2023
TI  - "Immuno-FlowFISH": Applications for Chronic Lymphocytic Leukemia.
PG  - 149-171
LID - 10.1007/978-1-0716-3020-4_9 [doi]
AB  - Imaging flow cytometry has the capacity to bridge the gap that currently exists 
      between the diagnostic tests that detect important phenotypic and genetic changes 
      in the clinical assessment of leukemia and other hematological malignancies or 
      blood-based disorders. We have developed an "Immuno-flowFISH" method that 
      leverages the quantitative and multi-parametric power of imaging flow cytometry 
      to push the limits of single-cell analysis. Immuno-flowFISH has been fully 
      optimized to detect clinically significant numerical and structural chromosomal 
      abnormalities (i.e., trisomy 12 and del(17p)) within clonal CD19/CD5+ CD3- 
      Chronic Lymphocytic Leukemia (CLL) cells in a single test. This integrated 
      methodology has greater accuracy and precision than standard fluorescence in situ 
      hybridization (FISH). We have detailed this immuno-flowFISH application with a 
      carefully catalogued workflow, technical instructions, and a repertoire of 
      quality control considerations to supplement the analysis of CLL. This 
      next-generation imaging flow cytometry protocol may provide unique advancements 
      and opportunities in the holistic cellular assessment of disease for both 
      research and clinical laboratory settings.
CI  - (c) 2023. Springer Science+Business Media, LLC, part of Springer Nature.
FAU - Hui, Henry Y L
AU  - Hui HYL
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, WA, Australia.
FAU - Erber, Wendy N
AU  - Erber WN
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, WA, Australia.
AD  - PathWest Laboratory Medicine, Nedlands, WA, Australia.
FAU - Fuller, Kathy A
AU  - Fuller KA
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, WA, Australia. 
      kathy.fuller@uwa.edu.au.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Humans
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Chromosome Aberrations
MH  - Trisomy
MH  - Flow Cytometry
OTO - NOTNLM
OT  - Chronic lymphocytic leukemia
OT  - Fluorescence in situ hybridization
OT  - Imaging flow cytometry
EDAT- 2023/04/19 12:41
MHDA- 2023/04/21 06:41
CRDT- 2023/04/19 11:24
PHST- 2023/04/21 06:41 [medline]
PHST- 2023/04/19 12:41 [pubmed]
PHST- 2023/04/19 11:24 [entrez]
AID - 10.1007/978-1-0716-3020-4_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2635:149-171. doi: 10.1007/978-1-0716-3020-4_9.