PMID- 37075347
OWN - NLM
STAT- MEDLINE
DCOM- 20230421
LR  - 20230427
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 56
DP  - 2023
TI  - Effect of propolis on Th2 and Th17 cells: interplay with EtxB- and LPS-treated 
      dendritic cells.
PG  - e12659
LID - S0100-879X2023000100628 [pii]
LID - 10.1590/1414-431X2023e12659 [doi]
LID - e12659
AB  - Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation 
      of T CD4+ cells into different profiles according to the nature of the antigen or 
      immunomodulator. Propolis is a resinous product made by bees that has numerous 
      pharmacological properties, including an immunomodulatory action. To assess 
      whether propolis can modulate the activation of CD4+ T cells by stimulating DCs 
      with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we 
      aimed to elucidate the mechanisms affected by propolis in the differential 
      activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene 
      expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and 
      IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher 
      lymphoproliferation compared with the control. Propolis induced GATA-3 expression 
      and, in combination with EtxB, maintained the baseline levels. Propolis alone or 
      in combination with LPS inhibited RORc expression. EtxB alone and in combination 
      with propolis increased IL-4 production. Propolis in combination with LPS 
      prevented LPS-induced IL-17A production. These results opened perspectives for 
      the study of biological events that may be favored by propolis by promoting Th2 
      activation or helping in the treatment of inflammatory conditions mediated by 
      Th17 cells.
FAU - Conti, B J
AU  - Conti BJ
AUID- ORCID: 0000-0001-7369-4910
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Santiago, K B
AU  - Santiago KB
AUID- ORCID: 0000-0001-7034-3840
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Cardoso, E O
AU  - Cardoso EO
AUID- ORCID: 0000-0002-4722-3733
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Conte, F L
AU  - Conte FL
AUID- ORCID: 0000-0001-6973-4465
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Golim, M A
AU  - Golim MA
AUID- ORCID: 0000-0002-4824-9441
AD  - Hemocentro de Botucatu, Faculdade de Medicina, Universidade Estadual Paulista, 
      Botucatu, SP, Brasil.
FAU - Cruz, M T
AU  - Cruz MT
AUID- ORCID: 0000-0001-9846-6754
AD  - Faculty of Pharmacy, Center for Neurosciences and Cellular Biology, University of 
      Coimbra, Coimbra, Portugal.
FAU - Sforcin, J M
AU  - Sforcin JM
AUID- ORCID: 0000-0003-3466-4895
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20230414
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Bacterial Toxins)
RN  - 0 (Lipopolysaccharides)
RN  - 9009-62-5 (Propolis)
RN  - 0 (Interleukin-17)
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - *Bacterial Toxins/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - *Propolis/pharmacology
MH  - Interleukin-17
MH  - Th17 Cells
MH  - *Escherichia coli Proteins/pharmacology
MH  - Dendritic Cells
MH  - Th2 Cells
PMC - PMC10125804
EDAT- 2023/04/19 18:41
MHDA- 2023/04/21 06:41
PMCR- 2023/04/14
CRDT- 2023/04/19 17:32
PHST- 2023/01/16 00:00 [received]
PHST- 2023/03/07 00:00 [accepted]
PHST- 2023/04/21 06:41 [medline]
PHST- 2023/04/19 18:41 [pubmed]
PHST- 2023/04/19 17:32 [entrez]
PHST- 2023/04/14 00:00 [pmc-release]
AID - S0100-879X2023000100628 [pii]
AID - 10.1590/1414-431X2023e12659 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2023 Apr 14;56:e12659. doi: 10.1590/1414-431X2023e12659. 
      eCollection 2023.